
NATURE . COM {
}
Title:
Phosphorylation of HDM2 by Akt | Oncogene
Description:
The HDM2 protein is a key regulator of the tumour suppressor, p53. Control of HDM2 function is critical for normal cell proliferation and stress responses, and it is becoming evident that multiple modifications of HDM2 can regulate its function within cells. In this study we show that HDM2 associated with the serine-threonine kinase, Akt, in response to growth factor stimulation of human primary cells. This association was concurrent with phosphorylation of Akt (at Ser 473), and resulted in elevated expression of HDM2 and enhanced nuclear localization. However, analysis of HDM2 proteins mutated at the consensus Akt recognition sites at serines 166 and 186 indicated that modification at these residues was not sufficient for the increased expression of the protein, which was blocked by the PI3 kinase inhibitor LY294002. Tryptic peptide and mutational analyses revealed evidence for an Akt phosphorylation site in HDM2 additional to the two consensus sites.
Website Age:
30 years and 10 months (reg. 1994-08-11).
Matching Content Categories {๐}
- Telecommunications
- Education
- Social Networks
Content Management System {๐}
What CMS is nature.com built with?
Custom-built
No common CMS systems were detected on Nature.com, and no known web development framework was identified.
Traffic Estimate {๐}
What is the average monthly size of nature.com audience?
๐ Phenomenal Traffic: 5M - 10M visitors per month
Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.
check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush
How Does Nature.com Make Money? {๐ธ}
Display Ads {๐ฏ}
The website utilizes display ads within its content to generate revenue. Check the next section for further revenue estimates.
Ads are managed by yourbow.com. Particular relationships are as follows:
Direct Advertisers (10)
google.com, pmc.com, doceree.com, yourbow.com, audienciad.com, onlinemediasolutions.com, advibe.media, aps.amazon.com, getmediamx.com, onomagic.comReseller Advertisers (38)
conversantmedia.com, rubiconproject.com, pubmatic.com, appnexus.com, openx.com, smartadserver.com, lijit.com, sharethrough.com, video.unrulymedia.com, google.com, yahoo.com, triplelift.com, onetag.com, sonobi.com, contextweb.com, 33across.com, indexexchange.com, media.net, themediagrid.com, adform.com, richaudience.com, sovrn.com, improvedigital.com, freewheel.tv, smaato.com, yieldmo.com, amxrtb.com, adyoulike.com, adpone.com, criteo.com, smilewanted.com, 152media.info, e-planning.net, smartyads.com, loopme.com, opera.com, mediafuse.com, betweendigital.comHow Much Does Nature.com Make? {๐ฐ}
Display Ads {๐ฏ}
$63,100 per month
Our estimates place Nature.com's monthly online earnings from display ads at $42,042 to $115,616.
Keywords {๐}
nature, cell, oncogene, access, biol, article, hdm, akt, vousden, mol, content, cookies, phosphorylation, ashcroft, cancer, privacy, woods, open, levine, data, journal, march, ludwig, chem, oren, research, function, advertising, analysis, information, subscribe, copeland, weber, karen, protein, cells, expression, institution, buy, george, chen, embo, sci, usa, shifman, author, permissions, site, optional, media,
Topics {โ๏ธ}
nature portfolio permissions reprints cancer research privacy policy advertising 1992 nature 358 nature social media author information authors dual-color immunohistochemical methods author correspondence 2d phosphoamino-acid analysis personal data springerlink instant access data protection permissions privacy human primary cells issue learn serine-threonine kinase explore content subscription content cancer marta falcicchiojake akt margaret ashcroft european economic area growth factor stimulation enhanced nuclear localization institutional subscriptions read ras-camp-pka surrey sm2 5ng ergโspink1 status full-length akt accepting optional cookies normal cell proliferation cell growth laboratory journals search log article ashcroft akt phosphorylation site manage preferences https detecting ergโpten hdm2 proteins mutated content article purchase access journal publish oliver weberย &ย karen article cite consensus sites optional cookies
Questions {โ}
- Pim kinases in hematological malignancies: where are we now and where are we going?
Schema {๐บ๏ธ}
WebPage:
mainEntity:
headline:Phosphorylation of HDM2 by Akt
description:The HDM2 protein is a key regulator of the tumour suppressor, p53. Control of HDM2 function is critical for normal cell proliferation and stress responses, and it is becoming evident that multiple modifications of HDM2 can regulate its function within cells. In this study we show that HDM2 associated with the serine-threonine kinase, Akt, in response to growth factor stimulation of human primary cells. This association was concurrent with phosphorylation of Akt (at Ser 473), and resulted in elevated expression of HDM2 and enhanced nuclear localization. However, analysis of HDM2 proteins mutated at the consensus Akt recognition sites at serines 166 and 186 indicated that modification at these residues was not sufficient for the increased expression of the protein, which was blocked by the PI3 kinase inhibitor LY294002. Tryptic peptide and mutational analyses revealed evidence for an Akt phosphorylation site in HDM2 additional to the two consensus sites.
datePublished:2002-03-22T00:00:00Z
dateModified:2002-03-22T00:00:00Z
pageStart:1955
pageEnd:1962
sameAs:https://doi.org/10.1038/sj.onc.1205276
keywords:
HDM2
Akt
p53
phosphorylation
Medicine/Public Health
general
Internal Medicine
Cell Biology
Human Genetics
Oncology
Apoptosis
image:
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig1_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig2_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig3_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig4_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig5_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig6_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig7_HTML.jpg
isPartOf:
name:Oncogene
issn:
1476-5594
0950-9232
volumeNumber:21
type:
Periodical
PublicationVolume
publisher:
name:Nature Publishing Group UK
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Margaret Ashcroft
affiliation:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research
address:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
type:PostalAddress
type:Organization
type:Person
name:Robert L Ludwig
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
type:Person
name:Douglas B Woods
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
type:Person
name:Terry D Copeland
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
type:Person
name:H Oliver Weber
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
type:Person
name:Elizabeth J MacRae
affiliation:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research
address:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
type:PostalAddress
type:Organization
type:Person
name:Karen H Vousden
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
email:[email protected]
type:Person
isAccessibleForFree:
hasPart:
isAccessibleForFree:
cssSelector:.main-content
type:WebPageElement
type:ScholarlyArticle
context:https://schema.org
ScholarlyArticle:
headline:Phosphorylation of HDM2 by Akt
description:The HDM2 protein is a key regulator of the tumour suppressor, p53. Control of HDM2 function is critical for normal cell proliferation and stress responses, and it is becoming evident that multiple modifications of HDM2 can regulate its function within cells. In this study we show that HDM2 associated with the serine-threonine kinase, Akt, in response to growth factor stimulation of human primary cells. This association was concurrent with phosphorylation of Akt (at Ser 473), and resulted in elevated expression of HDM2 and enhanced nuclear localization. However, analysis of HDM2 proteins mutated at the consensus Akt recognition sites at serines 166 and 186 indicated that modification at these residues was not sufficient for the increased expression of the protein, which was blocked by the PI3 kinase inhibitor LY294002. Tryptic peptide and mutational analyses revealed evidence for an Akt phosphorylation site in HDM2 additional to the two consensus sites.
datePublished:2002-03-22T00:00:00Z
dateModified:2002-03-22T00:00:00Z
pageStart:1955
pageEnd:1962
sameAs:https://doi.org/10.1038/sj.onc.1205276
keywords:
HDM2
Akt
p53
phosphorylation
Medicine/Public Health
general
Internal Medicine
Cell Biology
Human Genetics
Oncology
Apoptosis
image:
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig1_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig2_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig3_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig4_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig5_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig6_HTML.jpg
https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fsj.onc.1205276/MediaObjects/41388_2002_Article_BF1205276_Fig7_HTML.jpg
isPartOf:
name:Oncogene
issn:
1476-5594
0950-9232
volumeNumber:21
type:
Periodical
PublicationVolume
publisher:
name:Nature Publishing Group UK
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Margaret Ashcroft
affiliation:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research
address:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
type:PostalAddress
type:Organization
type:Person
name:Robert L Ludwig
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
type:Person
name:Douglas B Woods
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
type:Person
name:Terry D Copeland
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
type:Person
name:H Oliver Weber
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
type:Person
name:Elizabeth J MacRae
affiliation:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research
address:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
type:PostalAddress
type:Organization
type:Person
name:Karen H Vousden
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
email:[email protected]
type:Person
isAccessibleForFree:
hasPart:
isAccessibleForFree:
cssSelector:.main-content
type:WebPageElement
["Periodical","PublicationVolume"]:
name:Oncogene
issn:
1476-5594
0950-9232
volumeNumber:21
Organization:
name:Nature Publishing Group UK
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research
address:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
type:PostalAddress
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research
address:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
type:PostalAddress
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
ImageObject:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
name:Margaret Ashcroft
affiliation:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research
address:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
type:PostalAddress
type:Organization
name:Robert L Ludwig
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
name:Douglas B Woods
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
name:Terry D Copeland
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
name:H Oliver Weber
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
name:Elizabeth J MacRae
affiliation:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research
address:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
type:PostalAddress
type:Organization
name:Karen H Vousden
affiliation:
name:Regulation of Cell Growth Laboratory
address:
name:Regulation of Cell Growth Laboratory, Maryland, USA
type:PostalAddress
type:Organization
email:[email protected]
PostalAddress:
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
name:Regulation of Cell Growth Laboratory, Maryland, USA
name:Regulation of Cell Growth Laboratory, Maryland, USA
name:Regulation of Cell Growth Laboratory, Maryland, USA
name:Regulation of Cell Growth Laboratory, Maryland, USA
name:CRC, Centre for Cancer Therapeutics, The Institute of Cancer Research, UK
name:Regulation of Cell Growth Laboratory, Maryland, USA
WebPageElement:
isAccessibleForFree:
cssSelector:.main-content
External Links {๐}(27)
- How much revenue does https://link.springer.com/article/10.1038/sj.onc.1205276?utm_source=nature&utm_medium=referral&utm_campaign=buyArticle bring in?
- How much does https://citation-needed.springer.com/v2/references/10.1038/sj.onc.1205276?format=refman&flavour=references net monthly?
- How much revenue does https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Margaret%20Ashcroft produce monthly?
- Find out how much https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Margaret%20Ashcroft%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en earns monthly
- What's the income generated by https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Robert%20L%20Ludwig each month?
- https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Robert%20L%20Ludwig%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en's total income per month
- How much profit is https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Douglas%20B%20Woods making per month?
- What is the monthly revenue of https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Douglas%20B%20Woods%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en?
- Get to know https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Terry%20D%20Copeland's earnings
- How much revenue does https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Terry%20D%20Copeland%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en produce monthly?
- How much money does https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=H%20Oliver%20Weber generate?
- How much profit does https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22H%20Oliver%20Weber%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en generate?
- What's the monthly money flow for https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Elizabeth%20J%20MacRae?
- Financial intake of https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Elizabeth%20J%20MacRae%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en
- See how much https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Karen%20H%20Vousden makes per month
- Explore the financials of https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Karen%20H%20Vousden%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en
- How much does https://s100.copyright.com/AppDispatchServlet?title=Phosphorylation%20of%20HDM2%20by%20Akt&author=Margaret%20Ashcroft%20et%20al&contentID=10.1038%2Fsj.onc.1205276©right=Macmillan%20Publishers%20Limited&publication=0950-9232&publicationDate=2002-03-22&publisherName=SpringerNature&orderBeanReset=true bring in each month?
- How much does https://citation-needed.springer.com/v2/references/10.1038/sj.onc.1205276?format=refman&flavour=citation rake in every month?
- How much cash flow does https://doi.org/10.1186/s12929-022-00860-9 have monthly?
- How much revenue does https://doi.org/10.1038/s41420-020-00362-3 produce monthly?
- How much money does https://doi.org/10.1186/s13045-014-0095-z generate?
- What's the monthly income of https://doi.org/10.1038/modpathol.2012.234?
- How profitable is https://doi.org/10.1038/onc.2013.48?
- https://www.protocols.io/'s total income per month
- What's the total monthly financial gain of https://www.natureindex.com/?
- How much profit does http://www.naturechina.com make?
- How much profit is https://www.natureasia.com/ja-jp making per month?
Analytics and Tracking {๐}
- Google Tag Manager
Libraries {๐}
- Prism.js
- Zoom.js
Emails and Hosting {โ๏ธ}
Mail Servers:
- mxa-002c5801.gslb.pphosted.com
- mxb-002c5801.gslb.pphosted.com
Name Servers:
- pdns1.ultradns.net
- pdns2.ultradns.net
- pdns3.ultradns.org
- pdns4.ultradns.org
- pdns5.ultradns.info
- pdns6.ultradns.co.uk
CDN Services {๐ฆ}
- Crossref