BIOCONDUCTOR . ORG {
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Title:
Bioconductor - synapsis
Description:
Synapsis is a Bioconductor software package for automated (unbiased and reproducible) analysis of meiotic immunofluorescence datasets. The primary functions of the software can i) identify cells in meiotic prophase that are labelled by a synaptonemal complex axis or central element protein, ii) isolate individual synaptonemal complexes and measure their physical length, iii) quantify foci and co-localise them with synaptonemal complexes, iv) measure interference between synaptonemal complex-associated foci. The software has applications that extend to multiple species and to the analysis of other proteins that label meiotic prophase chromosomes. The software converts meiotic immunofluorescence images into R data frames that are compatible with machine learning methods. Given a set of microscopy images of meiotic spread slides, synapsis crops images around individual single cells, counts colocalising foci on strands on a per cell basis, and measures the distance between foci on any given strand.
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Keywords {🔍}
package, bioconductor, synapsis, version, packages, software, meiotic, analysis, release, synaptonemal, foci, images, source, mcneill, enter, install, license, binary, learn, developers, started, automate, doublestrand, break, repair, meiosis, immunofluorescence, cells, prophase, individual, complexes, measure, lucy, orcid, httpsbioconductororgpackagessynapsis, installation, start, documentation, system, text, details, bioc, stats, url, macos, synapsistgz, repository, git, clone, reports,
Topics {✒️}
org/packages/synapsis source repository joint galaxy/bioconductor conference double-strand break repair bioconductor release tgz source repository org/packages/synapsis source package bioconductor software package synapsis crops images meiotic immunofluorescence datasets release //code bioconductor support site meiotic spread slides central element protein machine learning methods individual single cells wayne crismani [rev script reference manual gz windows binary synaptonemal complex axis git clone https tgz macos binary lucy mcneill [aut details biocviews graphics system requirements counts colocalising foci microscopy images package installed package version 1 meiotic prophase started install packages synaptonemal complexes package synaptonemal complex macos binary software version identify cells //bioconductor bioconductor registration open primary functions physical length multiple species data frames cell basis cph] orcid ctb] orcid quietly = true
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