PMC . NCBI . NLM . NIH . GOV {
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We are analyzing https://pmc.ncbi.nlm.nih.gov/articles/PMC3486726/.
Title:
Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter - PMC
Description:
Mitochondria from diverse organisms are capable of transporting large amounts of Ca2+ via a ruthenium-red-sensitive, membrane-potential-dependent mechanism called the uniporter1–4. Although the uniporter’s biophysical properties have been studied ...
Website Age:
27 years and 8 months (reg. 1997-10-02).
Title:
Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter - PMC
Description:
Mitochondria from diverse organisms are capable of transporting large amounts of Ca2+ via a ruthenium-red-sensitive, membrane-potential-dependent mechanism called the uniporter1–4. Although the uniporter’s biophysical properties have been studied ...
Website Age:
27 years and 8 months (reg. 1997-10-02).
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- Science
- Education
- Technology & Computing
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Custom-built
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Traffic Estimate {📈}
What is the average monthly size of pmc.ncbi.nlm.nih.gov audience?
🌠 Phenomenal Traffic: 5M - 10M visitors per month
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Keywords {🔍}
mcu, mitochondrial, doi, fig, cells, uptake, protein, pubmed, google, scholar, mitochondria, micu, membrane, mouse, pmc, supplementary, calcium, hela, analysis, hek, uniporter, article, nature, data, performed, liver, free, proteins, silencing, cell, expressing, studies, transport, transmembrane, intact, expression, control, tissues, shmcu, sirna, nlm, coexpression, weight, complex, immunoblot, permeabilized, simcu, siluc, mice, respiratory,
Topics {✒️}
transfecting green-fluorescent-protein-tagged mcu mcu–v5-expressing hek-293 mitoplasts stably expressing sh-lacz membrane-potential-dependent mechanism called exogenous ligand-based mechanisms terminus v5-tagged version full-length mcu cdnas digitonin-solubilized mitochondria purified lentiviral shrna-expressing constructs pmc beta search immunoprecipitating flag-tagged mcu full-length mcu cdna protein-coupled receptors half-maximal inhibitory concentration mitochondria-targeted aequorin reporter9 mcu-mediated ca2+ uptake immunoprecipitating gfp-tagged mcu control sh-lacz cells sh-lacz control cells anti-v5 immunoprecipitations performed mitochondrial inter-membrane space lipid-based formulation optimized permeabilized hek-293 cells respiratory state transitions green fluorescent protein native gel electrophoresis blue native electrophoresis genome-wide mrna supplementary materials included conserved amino acids v5-tagged mcu genome-wide rna observed molecular weight apparent molecular weight si-mcu mice show alkaline carbonate extraction evolutionarily conserved duo untreated hek-293 cells larger complex disappears agonist-induced rises genome phylogenetic profiling computed genome-wide attenuates ca2+ activation notably respiratory flux c-termini facing calcium green-5n predicted transmembrane protein obtain similar results predicted transmembrane domains publisher site pdf
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