
PMC . NCBI . NLM . NIH . GOV {
}
Title:
Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics - PMC
Description:
A-kinase anchoring proteins (AKAPs) tether the cAMP-dependent protein kinase (PKA) and other signaling enzymes to distinct subcellular organelles. Using the yeast two-hybrid approach, we demonstrate that Rab32, a member of the Ras superfamily of ...
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Keywords {🔍}
rab, fig, protein, cells, rii, mitochondrial, proteins, pka, binding, mitochondria, pubmed, google, scholar, doi, cell, mutant, anchoring, expression, biol, kinase, rabtn, analysis, detected, domain, gtp, yeast, peptide, experiments, scott, assay, recombinant, data, pmc, akaps, molecular, helix, family, studies, membrane, bound, control, akap, nucleotide, residues, performed, cos, targeting, assessed, presented, antibody,
Topics {✒️}
nh2-terminal gst fusions bio-rad laboratories recombinant wild-type gst–rab32 affinity-purified anti-rab32 antibody riiα dimerization/akap-binding domain stress-activated protein kinase dynamin-related protein drp1 hypervariable c-terminal domain genbank/embl/ddbj accession dynamin-related gtpase drp1 cooh-terminal hypervariable domain wild-type cells exhibited a-kinase anchor proteins flag-tagged wild-type a-kinase anchoring protein small g-protein location pmc beta search a-kinase anchoring proteins cooh-terminal hypervariable region gtp binding–deficient mutant gtp binding–deficient form pmc copyright notice sepharose-conjugated antiflag antibody full-length rab32 cdna cooh-terminal cysteine pair camp-dependent protein kinase wild-type rab32 codistributed fitc-conjugated antiflag antibody small gtp-binding proteins wiskott-aldrich syndrome protein solid-phase peptide array cooh-terminal lipid modification �signal-organizing” molecules ensure cloning full-length rab32 32p-radiolabeled 113-bp probe cooh-terminal cysteine residues pka binding–deficient mutant monoclonal anti-riiα antibody gtp binding–defective mutant cy5-conjugated secondary antibodies akap-lbc abolishes interaction triton x-100–soluble extracts auto-spot robot asp222 free rab protein studying protein-protein interactions solid-phase binding assay drp-1–interfering mutants results anti-rii monoclonal antibody regulate membrane fusion/fission purified recombinant gst–rab32
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