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Keywords {🔍}

complex, doi, sno, mitochondrial, pubmed, google, scholar, snitrosation, figure, mitochondria, gsno, inhibition, protein, gel, analysis, column, content, results, nitric, oxide, activity, chemiluminescent, bluenative, approx, respiratory, buffer, samples, treatment, free, pmc, mass, proteins, peak, addition, formation, shows, chromatography, assay, fraction, superose, detection, chain, control, heart, data, shown, electrophoresis, methods, biotinswitch, ipc,

Topics {✒️}

matrix-assisted laser-desorption ionization–time cub/haem-a3 site argon-fed purge vessel fas-induced caspase denitrosylation enhanced s-nitroso-albumin formation mass spectra [maldi-tof dimensional blue-native electrophoresis low-molecular-mass thiols blue-native gel electrophoresis pmc beta search established blue-native method high-molecular-mass protein myocardial ischemia-reperfusion injury detect s-nitrosation relied typical blue-native gel 900 kda l-shaped form small sno-induced increase cardiac ischemia-reperfusion injury detects protein-bound sno protein-bound sno saturates detect s-nitrosated targets identify s-nitrosated peptides streptavidin–horseradish peroxidase s-nitrosation-dependent mechanism molecular mass markers bio-rad laboratories single s-nitrosated peptide s-nitrosothiol-induced inhibition free radical generation homogenization/centrifugation process repeated s-nitrosated proteins excised protein-bound sno detected blue-native gel blue native gel peptide mass fingerprinting maldi-tof-ms publisher site pdf license information pmcid blue-native gels biotin switch method free radical res entire experiment including s-nitroso adduct intra-mitochondrial conditions low staining intensity direct molecular evidence free radical biol ischaemia-reperfusion injury [28] folin-phenol method [29] regulates superoxide production

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