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Title:
Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors | SpringerLink
Description:
Matrix metalloproteinases (MMPs) are proteolytic enzymes that degrade various components of the extracellular matrix (ECM) and play a role in tissue remodeling. Changes in MMPs have been observed in cancer, connective tissue disorders, and vascular disease, and both...
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Schema {🗺️}

ScholarlyArticle:
      headline:Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors
      pageEnd:102
      pageStart:79
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-7111-4.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Zymography
         isbn:
            978-1-4939-7111-4
            978-1-4939-7109-1
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Zongli Ren
            affiliation:
                  name:Brigham and Women’s Hospital and Harvard Medical School
                  address:
                     name:Vascular Surgery Research Laboratory, Division of Vascular and Endovascular Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Juanjuan Chen
            affiliation:
                  name:Brigham and Women’s Hospital and Harvard Medical School
                  address:
                     name:Vascular Surgery Research Laboratory, Division of Vascular and Endovascular Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Raouf A. Khalil
            affiliation:
                  name:Brigham and Women’s Hospital and Harvard Medical School
                  address:
                     name:Vascular Surgery Research Laboratory, Division of Vascular and Endovascular Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Blood vessels, Uterus, Extracellular matrix, Matrix metalloproteinase, TIMP
      description:Matrix metalloproteinases (MMPs) are proteolytic enzymes that degrade various components of the extracellular matrix (ECM) and play a role in tissue remodeling. Changes in MMPs have been observed in cancer, connective tissue disorders, and vascular disease, and both endogenous tissue inhibitors of MMPs (TIMPs) and synthetic MMP inhibitors (MMPIs) have been evaluated as modulators of MMP activity in various biological systems. Zymography is a simple technique that is commonly used to assess MMP activity and the efficacy of MMPIs. Also, reverse zymography is a modified technique to study the activity of endogenous TIMPs. However, problems are often encountered during the zymography procedure, which could interfere with accurate assessment of MMP activity in control specimens, and thus make it difficult to determine the pathological changes in MMPs and their responsiveness to MMPIs. Simplified protocols for preparation of experimental solutions, tissue preparation, regular and reverse zymography procedures, and zymogram analysis are presented. Additional helpful tips to troubleshoot problems in the zymography technique and to enhance the quality of the zymograms should make it more feasible to determine the changes in MMPs and assess the efficacy of MMPIs in modulating MMP activity in various biological systems and pathological conditions.
      datePublished:2017
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      isbn:
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         978-1-4939-7109-1
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      name:Springer New York
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         type:ImageObject
      name:Brigham and Women’s Hospital and Harvard Medical School
      address:
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Person:
      name:Zongli Ren
      affiliation:
            name:Brigham and Women’s Hospital and Harvard Medical School
            address:
               name:Vascular Surgery Research Laboratory, Division of Vascular and Endovascular Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      name:Juanjuan Chen
      affiliation:
            name:Brigham and Women’s Hospital and Harvard Medical School
            address:
               name:Vascular Surgery Research Laboratory, Division of Vascular and Endovascular Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      name:Raouf A. Khalil
      affiliation:
            name:Brigham and Women’s Hospital and Harvard Medical School
            address:
               name:Vascular Surgery Research Laboratory, Division of Vascular and Endovascular Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
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      name:Vascular Surgery Research Laboratory, Division of Vascular and Endovascular Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA
      name:Vascular Surgery Research Laboratory, Division of Vascular and Endovascular Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA
      name:Vascular Surgery Research Laboratory, Division of Vascular and Endovascular Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, USA
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