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Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein C | SpringerLink
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Capillary electrophoresis-based SELEX (CE-SELEX) is an efficient technique for the isolation of aptamers binding to a wide range of target molecules. CE-SELEX has a number of advantages over conventional SELEX procedures such as the selection of aptamers can be...
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Keywords {🔍}
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Topics {✒️}
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ScholarlyArticle:
headline:Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein C
pageEnd:75
pageStart:61
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3197-2.jpg
genre:
Springer Protocols
isPartOf:
name:Nucleic Acid Aptamers
isbn:
978-1-4939-3197-2
978-1-4939-3196-5
type:Book
publisher:
name:Springer New York
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Nasim Shahidi Hamedani
affiliation:
name:University of Bonn Medical Centre
address:
name:Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
type:PostalAddress
type:Organization
type:Person
name:Jens Müller
affiliation:
name:University of Bonn Medical Centre
address:
name:Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:
CE-SELEX
,
Aptamer
,
Capillary electrophoresis
,
SELEX
,
Activated protein C
description:Capillary electrophoresis-based SELEX (CE-SELEX) is an efficient technique for the isolation of aptamers binding to a wide range of target molecules. CE-SELEX has a number of advantages over conventional SELEX procedures such as the selection of aptamers can be performed on non-immobilized targets, usually within a fewer number of selection cycles. Here we describe a complete procedure of CE-SELEX using activated protein C (APC) as the target protein.
datePublished:2016
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name:University of Bonn Medical Centre
address:
name:Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
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name:Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
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name:Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
type:PostalAddress
type:Organization
name:Jens Müller
affiliation:
name:University of Bonn Medical Centre
address:
name:Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
type:PostalAddress
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email:[email protected]
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name:Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
name:Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
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