We are analyzing https://link.springer.com/article/10.1186/s13578-018-0202-x.

Title:
Characterization and selective incorporation of small non-coding RNAs in non-small cell lung cancer extracellular vesicles | Cell & Bioscience
Description:
Background Extracellular vesicles (EVs) play important roles in intercellular communication through the delivery of their cargoes, which include proteins, lipids, and RNAs. Increasingly, multiple studies have reported the association between EV small non-coding RNAs and cancer, due to their regulatory functions in gene expression. Hence, analysis of the features of small non-coding RNA expression and their incorporation into EVs is important for cancer research. Results We performed deep sequencing to investigate the expression of small RNAs in plasma EVs from lung adenocarcinoma (ADC) patients, lung squamous cell carcinoma (SQCC) patients, and healthy controls. Then, eighteen differently expressed miRNAs in plasma EVs was validated by QRT-PCR. The small RNA expression profiles of plasma EVs were different among lung ADC, SQCC patients, and healthy controls. And many small RNAs, including 5′ YRNA hY4-derived fragments, miR-451a, miR-122-5p, miR-20a-5p, miR-20b-5p, miR-30b-5p, and miR-665, were significantly upregulated in non-small cell lung cancer (NSCLC) EVs. And the cell viability assays indicated that hY4-derived fragments inhibited the proliferation of lung cancer cell A549. By comparing the cellular and EV expression levels of six miRNAs in NSCLC cells, we found that miR-451a and miR-122-5p were significantly downregulated in NSCLC cell lysates, while significantly upregulated in NSCLC EVs. Conclusions The differently expressed EV small RNAs may serve as potential circulating biomarkers for the diagnosis of NSCLC. Particularly, YRNA hY4-derived fragments can serve as a novel class of biomarkers, which function as tumor suppressors in NSCLC. Additionally, miR-451a and miR-122-5p may be sorted into NSCLC EVs in a selective manner.
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Keywords {🔍}

evs, pubmed, nsclc, small, article, cancer, google, scholar, mirnas, rna, cell, expression, sqcc, lung, adc, plasma, cas, rnas, cells, fragments, ctrl, significantly, exosomes, mirna, central, mira, groups, analysis, fig, exosomal, human, mirp, results, patients, mirbp, yrna, found, noncoding, upregulated, wang, samples, gene, sequencing, reads, data, profiles, size, targets, distribution, healthy,

Topics {✒️}

trf-leu-cag trna fragment large-scale clip-seq data fitc-conjugated anti-cd63 sqcc-specific mirnas mir-10b-5p rna-seq fold-change values directly targeting c-myc article download pdf rna-binding protein-dependent pathway extracellular vesicle uptake small-cell lung cancer tween/tris buffered saline full size image additional figures s1–s7 hy4-derived fragments functioned tumor suppressor mir-193a rab27-dependent exosome release development-related biological processes hy4 rna-derived fragments cancer-specific expression profiles colorectal cancer-specific mortality cancer cell-derived exosomes tumor-derived exosomal mirna mvp-mediated exosomal sorting protein-rna interaction networks particle size distribution epithelial-mesenchymal transition inhibitor qrt-pcr results showing form rna–protein complexes yrna hy4-derived fragments hy4-derived fragments inhibited tumor-derived exosomal mirnas transmission electron microscopy mir-320/mir-574-3p/rnu6-1 play important roles performed high-throughput sequencing ev-mediated intercellular communication mirneasy serum/plasma spike qrt-pcr validation results extracellular circulating microrna sybr-based qrt-pcr table 4 rna-seq results transmission electron microscope squamous cell carcinoma tumor suppressor mir-122-5p full access qrt-pcr results showed real-time pcr control cel-mir-39 mimic mir-122-5p inhibits metastasis y-box protein 1

Questions {❓}

Differences between squamous cell carcinoma and adenocarcinoma of the lung: are adenocarcinoma and squamous cell carcinoma prognostically equal?

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         description:Extracellular vesicles (EVs) play important roles in intercellular communication through the delivery of their cargoes, which include proteins, lipids, and RNAs. Increasingly, multiple studies have reported the association between EV small non-coding RNAs and cancer, due to their regulatory functions in gene expression. Hence, analysis of the features of small non-coding RNA expression and their incorporation into EVs is important for cancer research. We performed deep sequencing to investigate the expression of small RNAs in plasma EVs from lung adenocarcinoma (ADC) patients, lung squamous cell carcinoma (SQCC) patients, and healthy controls. Then, eighteen differently expressed miRNAs in plasma EVs was validated by QRT-PCR. The small RNA expression profiles of plasma EVs were different among lung ADC, SQCC patients, and healthy controls. And many small RNAs, including 5′ YRNA hY4-derived fragments, miR-451a, miR-122-5p, miR-20a-5p, miR-20b-5p, miR-30b-5p, and miR-665, were significantly upregulated in non-small cell lung cancer (NSCLC) EVs. And the cell viability assays indicated that hY4-derived fragments inhibited the proliferation of lung cancer cell A549. By comparing the cellular and EV expression levels of six miRNAs in NSCLC cells, we found that miR-451a and miR-122-5p were significantly downregulated in NSCLC cell lysates, while significantly upregulated in NSCLC EVs. The differently expressed EV small RNAs may serve as potential circulating biomarkers for the diagnosis of NSCLC. Particularly, YRNA hY4-derived fragments can serve as a novel class of biomarkers, which function as tumor suppressors in NSCLC. Additionally, miR-451a and miR-122-5p may be sorted into NSCLC EVs in a selective manner.
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      name:Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China
      name:Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China
      name:School of Basic Medical Sciences, Wuhan University, Wuhan, People’s Republic of China
      name:Department of Radiation Oncology, Hubei Cancer Hospital, Wuhan, People’s Republic of China
      name:Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of China
      name:Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China
      name:Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China

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