We are analyzing https://link.springer.com/article/10.1186/s13568-014-0092-1.

Title:
A psychrotolerant strain of Serratia marcescens (MTCC 4822) produces laccase at wide temperature and pH range | AMB Express
Description:
A psychrotolerant bacterial strain of Serratia marcescens, originally isolated from a glacial site in Indian Himalayan Region (IHR), has been investigated for laccase production under different culture conditions. The bacterial strain was found to grow between 4 to 45°C (opt. 25°C) and 3 to 14 pH (opt. 5 pH) on prescribed growth medium, coinciding with production of laccase in laccase producing medium. However, the production of laccase was more consistent toward alkaline pH. Laccase enzyme was partially purified using gel filtration chromatography. The molecular mass of laccase was determined ~53 kDa on native PAGE. The Km and Vmax values were determined to be 0.10 mM and 50.00 μM min−1, respectively, with ABTS. Inoculum size (4.0% v/v at 1.5 O.D.) resulted in significantly higher production of laccase. Carbon and nitrogen sources also affected the laccase production significantly. All the carbon sources enhanced laccase production, xylose being the best enhancer (P < 0.01). Among nitrogen sources, organic sources were found to act as inhibitors (P < 0.01), and among the in-organic sources only sodium nitrate enhanced the laccase production. Low molecular weight organic solvents significantly (P < 0.01) enhanced laccase production up to 24 h of incubation with a decline in later incubation period. Production of laccase by the psychrotolerant bacterium in wide range of temperature and pH is likely to have inference in biotechnological processes.
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Keywords {🔍}

laccase, production, article, enzyme, google, scholar, conditions, marcescens, sources, temperature, size, serratia, growth, medium, bacterium, bacterial, pandey, inoculum, molecular, organic, incubation, cas, strain, nitrogen, culture, solvents, characterization, laccases, activity, table, phase, figure, microbiol, range, isolated, significantly, purification, physicochemical, study, full, carbon, effect, pubmed, min, higher, biotechnological, applications, biotechnol, mtcc, abts,

Topics {✒️}

increase–decrease-increase phenomenon pertaining indian himalayan region cold-tolerant phosphate-solubilizing microorganisms indian central himalaya kusum dhakar & anita pandey marcescens biovar a2/a6 article download pdf lineweaver burk plot full size image peculiar physico-chemical characters low molecular weight dye effluent decolorization extreme physico-chemical conditions privacy choices/manage cookies pseudomonas corrugata inoculation bacillus megaterium strain bacillus aerius sp3 de la fuente cold tolerant pgprs full access allotted accession number extracellular enzymes produced environ sci health inorganic nitrogen sources organic sources acted enterococcus faecium a2 related subjects sodium nitrate enhanced citrate- phosphate buffer citrate-phosphate buffer physico-chemical factors serratia marcescens n4-5 psychrotolerant bacterial strain enhanced cell growth european economic area naturally evolved recalcitrance 10-fold serial dilutions raising fresh cultures 3-ethylbenzothiazoline-6-sulphonic acid post-hoc tukey intracellularly located enzymes play crucial role enterobacter aerogenes nbo2 lens esculenta moench poutou pinales ra delgado- boada jm thermo-alkali-stable obtain permission directly final population size metal tolerant laccase

Schema {🗺️}

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         headline:A psychrotolerant strain of Serratia marcescens (MTCC 4822) produces laccase at wide temperature and pH range
         description:A psychrotolerant bacterial strain of Serratia marcescens, originally isolated from a glacial site in Indian Himalayan Region (IHR), has been investigated for laccase production under different culture conditions. The bacterial strain was found to grow between 4 to 45°C (opt. 25°C) and 3 to 14 pH (opt. 5 pH) on prescribed growth medium, coinciding with production of laccase in laccase producing medium. However, the production of laccase was more consistent toward alkaline pH. Laccase enzyme was partially purified using gel filtration chromatography. The molecular mass of laccase was determined ~53 kDa on native PAGE. The Km and Vmax values were determined to be 0.10 mM and 50.00 μM min−1, respectively, with ABTS. Inoculum size (4.0% v/v at 1.5 O.D.) resulted in significantly higher production of laccase. Carbon and nitrogen sources also affected the laccase production significantly. All the carbon sources enhanced laccase production, xylose being the best enhancer (P < 0.01). Among nitrogen sources, organic sources were found to act as inhibitors (P < 0.01), and among the in-organic sources only sodium nitrate enhanced the laccase production. Low molecular weight organic solvents significantly (P < 0.01) enhanced laccase production up to 24 h of incubation with a decline in later incubation period. Production of laccase by the psychrotolerant bacterium in wide range of temperature and pH is likely to have inference in biotechnological processes.
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      headline:A psychrotolerant strain of Serratia marcescens (MTCC 4822) produces laccase at wide temperature and pH range
      description:A psychrotolerant bacterial strain of Serratia marcescens, originally isolated from a glacial site in Indian Himalayan Region (IHR), has been investigated for laccase production under different culture conditions. The bacterial strain was found to grow between 4 to 45°C (opt. 25°C) and 3 to 14 pH (opt. 5 pH) on prescribed growth medium, coinciding with production of laccase in laccase producing medium. However, the production of laccase was more consistent toward alkaline pH. Laccase enzyme was partially purified using gel filtration chromatography. The molecular mass of laccase was determined ~53 kDa on native PAGE. The Km and Vmax values were determined to be 0.10 mM and 50.00 μM min−1, respectively, with ABTS. Inoculum size (4.0% v/v at 1.5 O.D.) resulted in significantly higher production of laccase. Carbon and nitrogen sources also affected the laccase production significantly. All the carbon sources enhanced laccase production, xylose being the best enhancer (P < 0.01). Among nitrogen sources, organic sources were found to act as inhibitors (P < 0.01), and among the in-organic sources only sodium nitrate enhanced the laccase production. Low molecular weight organic solvents significantly (P < 0.01) enhanced laccase production up to 24 h of incubation with a decline in later incubation period. Production of laccase by the psychrotolerant bacterium in wide range of temperature and pH is likely to have inference in biotechnological processes.
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         Organic solvents
         Indian Himalayan Region (IHR)
         Microbiology
         Microbial Genetics and Genomics
         Biotechnology
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