We are analyzing https://link.springer.com/article/10.1186/s13287-020-01933-y.

Title:
Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth | Stem Cell Research & Therapy
Description:
Millions suffer from skin diseases. Functional interfollicular epidermal stem cells are needed in skin therapy or drug screening in vitro. We obtained functional interfollicular epidermal stem cells with intact stemness and cell junctions by treating them with Wnt3a. Moreover, epidermal stem cell-derived extracellular vesicles were useful in epidermal cell growth. Finally, functional epidermal 3D organoids with polarity were cultured using Wnt3a and the supernatant derived from interfollicular epidermal stem cells and fresh medium in a 1:1 ratio. These results provide novel directions for the improvement of skin organoids and their potential in clinical application.
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Keywords {🔍}

stem, extracellular, cells, epidermal, cell, epscs, article, vesicles, google, scholar, skin, epscderived, cat, fig, cas, proteins, human, culture, wnta, analysis, development, signaling, epidermis, epsc, itgb, organoids, results, biological, function, including, wnt, proliferation, itga, krt, role, xav, data, growth, protein, tissue, differentiation, involved, layer, matrix, ecm, abcam, basal, important, min, epithelial,

Topics {✒️}

mediate cell-cell communication epsc-derived extracellular vesicles extracellular vesicle-free fbs cell adhesion molecules wnt/β-catenin signaling pathway n-terminal plakin domain avβ3/pi3k/akt/enos/ cell-specific extracellular vesicles alpha-1-microglobulin/bikunin precursor cell-matrix junction markers ms/ms raw files systems biology approach cell-cell junction markers basic layers—epidermal layer cell-matrix junction marker article download pdf ecm-digesting matrix metallopeptidases stem cell-derived proteome profile wnt/β-catenin signaling epidermal stem cells pre-warmed trypsin/edta extracellular vesicle analysis wnt/β-catenin pathway epidermal stem cell extracellular vesicle lysates wnt/β-catenin inhibitor derived extracellular vesicles proteome-wide protein quantification ling leng label-free proteomic analysis extracellular vesicle proteins epsc-derived vesicles full access mol cell proteomics advanced dmem/f12 medium extracellular vesicle isolation national key research stem cell industry stem cell signaling skin stem cells regulates epsc development bovine extracellular vesicles specialized extracellular matrix basal stem cells stem cell transplantation stem cell control cell-cell junction stem cell differentiation proteomics dataset supporting

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         headline:Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth
         description:Millions suffer from skin diseases. Functional interfollicular epidermal stem cells are needed in skin therapy or drug screening in vitro. We obtained functional interfollicular epidermal stem cells with intact stemness and cell junctions by treating them with Wnt3a. Moreover, epidermal stem cell-derived extracellular vesicles were useful in epidermal cell growth. Finally, functional epidermal 3D organoids with polarity were cultured using Wnt3a and the supernatant derived from interfollicular epidermal stem cells and fresh medium in a 1:1 ratio. These results provide novel directions for the improvement of skin organoids and their potential in clinical application.
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         dateModified:2020-09-23T00:00:00Z
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            Extracellular vesicle
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            Proteomics
            Epidermal development
            Extracellular matrix
            Stem Cells
            Cell Biology
            Regenerative Medicine/Tissue Engineering
            Biomedical Engineering and Bioengineering
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      headline:Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth
      description:Millions suffer from skin diseases. Functional interfollicular epidermal stem cells are needed in skin therapy or drug screening in vitro. We obtained functional interfollicular epidermal stem cells with intact stemness and cell junctions by treating them with Wnt3a. Moreover, epidermal stem cell-derived extracellular vesicles were useful in epidermal cell growth. Finally, functional epidermal 3D organoids with polarity were cultured using Wnt3a and the supernatant derived from interfollicular epidermal stem cells and fresh medium in a 1:1 ratio. These results provide novel directions for the improvement of skin organoids and their potential in clinical application.
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         Extracellular vesicle
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         Epidermal development
         Extracellular matrix
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         Cell Biology
         Regenerative Medicine/Tissue Engineering
         Biomedical Engineering and Bioengineering
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      name:State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Life Omics, Beijing, China
      name:Basic Medical School, Anhui Medical University, Hefei, China
      name:Stem Cell and Regenerative Medicine Lab, Department of Medical Science Research Center, Translational Medicine Center, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China

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