We are analyzing https://link.springer.com/article/10.1186/s13104-017-2715-5.

Title:
TNF-α-induced miR-155 regulates IL-6 signaling in rheumatoid synovial fibroblasts | BMC Research Notes
Description:
Background MicroRNAs (miRNAs) are important regulators of a variety of inflammatory mediators. The present study was undertaken to elucidate the role of miRNAs in the rheumatoid cytokine network. Methods We analyzed miRNA expression in rheumatoid synovial fibroblasts (RASFs). miRNA array-based screening was used to identify miRNAs differentially expressed between tumor necrosis factor-α (TNF-α)-activated RASFs and untreated RASFs. Transfection of RASFs with miR-155 was used to analyze the function of miR-155. Real-time polymerase chain reaction (PCR) was used to measure the levels of miR-155 in RASFs. Results miRNA microarray analysis revealed that miR-155-5p was the most highly induced miRNA in TNF-α-stimulated RASFs. TNF-α-induced miR-155 expression in RASFs was time-dependent and TNFα dose-dependent, whereas, IL-6 stimulation did not affect miR-155 expression in RASFs. Transfection of miR-155 mimics into RASFs resulted in the decrease JAK2/STAT3 phosphorylation in IL-6-treated RASFs. Conclusions The current results demonstrate that TNF-α modulated miRNA expressions in RASFs. Our data showed that miR-155, which is highly induced by TNF-α stimulation, inhibits IL-6-mediated JAK2/STAT3 activation in RASFs. These findings suggest that miR-155 contributes to the cross-regulation between TNF-α and IL-6-mediated inflammatory pathways in RA.
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Keywords {🔍}

rasfs, rheumatoid, article, expression, synovial, tnfα, pubmed, mirna, google, scholar, signaling, cas, mirnas, inflammatory, arthritis, fibroblasts, cytokine, data, pathway, jak, research, stat, analysis, patients, isolated, japan, stimulated, silr, stimulation, phosphorylation, cells, signal, ngml, analyzed, mirp, jakstat, activation, performed, microrna, experiments, fig, rev, nagasaki, tnfαinduced, study, ilmediated, cytokines, demonstrated, control, shown,

Topics {✒️}

tumor necrosis factor-α tnf-α-triggered cytokine signaling regulating nf-κb signaling il-6-mediated jaks/stat3 activation tnf-α-induced mir-155-5p tnf-α-exposed rheumatoid synoviocytes tnf-α-mediated epigenetic modulation tnf-α-induced rheumatoid synovium il-6-induced jak2/stat3 activation tnf-α-induced mir-155 expression article download pdf 3d-gene extraction software mirna array-based screening tnf-α-induced proliferation tnf-α-stimulated rasfs isolated typical multi-functional mirna il-6-mediated inflammatory cascade microrna-mediated gene silencing preventing jak2/sta3 activation pivotal proinflammatory factor il-6 trans-signaling system anti-tnf alpha therapy phospho-specific anti-jaks suppressing jak2/stat3 activation preventing jak2/stat3 activation il-6-mediated inflammatory pathways negative feed-back loop tnf-α-activated rasfs tnf-α-stimulated rasfs jak/stat pathway induces full access quantitative real-time pcr il-6/soluble il-6 receptor synoviocytes maintain tumor clinical research center tnf-α-induced scientist research c26461481 practical research project decrease jak2/stat3 phosphorylation transcription tnf-α inflammatory cytokines cascades interleukin-1 signaling pathway tnf-α modulates related subjects 3d-gene scanner 3000 important factor tnf-α stimulated privacy choices/manage cookies rheumatoid synovial fibroblasts tnf-α/il-6

Questions {❓}

Anti-TNF alpha therapy of rheumatoid arthritis: what have we learned?

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WebPage:
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         headline:TNF-α-induced miR-155 regulates IL-6 signaling in rheumatoid synovial fibroblasts
         description:MicroRNAs (miRNAs) are important regulators of a variety of inflammatory mediators. The present study was undertaken to elucidate the role of miRNAs in the rheumatoid cytokine network. We analyzed miRNA expression in rheumatoid synovial fibroblasts (RASFs). miRNA array-based screening was used to identify miRNAs differentially expressed between tumor necrosis factor-α (TNF-α)-activated RASFs and untreated RASFs. Transfection of RASFs with miR-155 was used to analyze the function of miR-155. Real-time polymerase chain reaction (PCR) was used to measure the levels of miR-155 in RASFs. miRNA microarray analysis revealed that miR-155-5p was the most highly induced miRNA in TNF-α-stimulated RASFs. TNF-α-induced miR-155 expression in RASFs was time-dependent and TNFα dose-dependent, whereas, IL-6 stimulation did not affect miR-155 expression in RASFs. Transfection of miR-155 mimics into RASFs resulted in the decrease JAK2/STAT3 phosphorylation in IL-6-treated RASFs. The current results demonstrate that TNF-α modulated miRNA expressions in RASFs. Our data showed that miR-155, which is highly induced by TNF-α stimulation, inhibits IL-6-mediated JAK2/STAT3 activation in RASFs. These findings suggest that miR-155 contributes to the cross-regulation between TNF-α and IL-6-mediated inflammatory pathways in RA.
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            Interleukin-6
            MicroRNAs
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            Synovial fibroblasts
            Tumor necrosis factor-α
            Biomedicine
            general
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            Life Sciences
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      headline:TNF-α-induced miR-155 regulates IL-6 signaling in rheumatoid synovial fibroblasts
      description:MicroRNAs (miRNAs) are important regulators of a variety of inflammatory mediators. The present study was undertaken to elucidate the role of miRNAs in the rheumatoid cytokine network. We analyzed miRNA expression in rheumatoid synovial fibroblasts (RASFs). miRNA array-based screening was used to identify miRNAs differentially expressed between tumor necrosis factor-α (TNF-α)-activated RASFs and untreated RASFs. Transfection of RASFs with miR-155 was used to analyze the function of miR-155. Real-time polymerase chain reaction (PCR) was used to measure the levels of miR-155 in RASFs. miRNA microarray analysis revealed that miR-155-5p was the most highly induced miRNA in TNF-α-stimulated RASFs. TNF-α-induced miR-155 expression in RASFs was time-dependent and TNFα dose-dependent, whereas, IL-6 stimulation did not affect miR-155 expression in RASFs. Transfection of miR-155 mimics into RASFs resulted in the decrease JAK2/STAT3 phosphorylation in IL-6-treated RASFs. The current results demonstrate that TNF-α modulated miRNA expressions in RASFs. Our data showed that miR-155, which is highly induced by TNF-α stimulation, inhibits IL-6-mediated JAK2/STAT3 activation in RASFs. These findings suggest that miR-155 contributes to the cross-regulation between TNF-α and IL-6-mediated inflammatory pathways in RA.
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         Cytokines
         Interleukin-6
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         Synovial fibroblasts
         Tumor necrosis factor-α
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         general
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         Life Sciences
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      name:Department of Rheumatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

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