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Keywords {🔍}

celseq, cells, cell, file, fig, genes, sequencing, additional, singlecell, article, number, methods, protocol, expression, method, analysis, data, sensitivity, mouse, gfp, smartseq, rna, primer, library, transcripts, original, detected, step, efficiency, reads, performed, single, cdna, umi, primers, pubmed, rnaseq, gene, figure, pipeline, sample, comparison, ligation, illumina, synthesis, read, barcodes, fluidigm, variation, google,

Topics {✒️}

dmem/10 % fbs/1 % pen-strep/1 % l-glutamine single-cell rna-seq analysis high-throughput sequencing data enabling highly-multiplexed analyses orit rozenblatt-rosen enabling highly-multiplexed analysis single-cell transcriptomics applied itai yanai highly multiplex rna-seq medical research israel-canada fluidigm c1-enabled cell-barcoding truseq small-rna kit single-cell rna-seq single-cell transcriptional landscape double stranded dna single-cell sequencing experiments mouse fibroblast cells mouse dendritic cells fast gapped-read alignment single-cell transcriptomics template-switch step full-length mrna-seq cyclinb1-gfp fusion reporter produces full-length transcripts avoid low-complexity barcodes /yanailab/cel-seq-pipeline multiplexed linear amplification reverse transcription cel-seq2 cel-seq article download pdf messageamp ii enzyme recessive fibroblast variants illumina adaptor directly library preparation step original cel-seq protocol hadassah medical center single sequencing error single-cell levels detected ercc spike-ins detailed cel-seq2 protocol single-cell method privacy choices/manage cookies ligation step technion genome center full transcript sequencing showed high coefficient superscript ii single library construction template switching mechanism reverse-transcribed mrna

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               type:PostalAddress
            type:Organization
      name:Itai Yanai
      affiliation:
            name:Technion – Israel Institute of Technology
            address:
               name:Department of Biology, Technion – Israel Institute of Technology, Haifa, Israel
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Biology, Technion – Israel Institute of Technology, Haifa, Israel
      name:Department of Biology, Technion – Israel Institute of Technology, Haifa, Israel
      name:Department of Biology, Technion – Israel Institute of Technology, Haifa, Israel
      name:Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
      name:Department of Biology, Technion – Israel Institute of Technology, Haifa, Israel
      name:Department of Biology, Technion – Israel Institute of Technology, Haifa, Israel
      name:Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, USA
      name:Department of Biology, MIT, Cambridge, USA
      name:Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, USA
      name:Fluidigm Corporation, South San Francisco, USA
      name:Fluidigm Corporation, South San Francisco, USA
      name:Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, USA
      name:Department of Biology, MIT, Cambridge, USA
      name:Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, USA
      name:Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
      name:Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, USA
      name:Department of Biology, MIT, Cambridge, USA
      name:Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, USA
      name:Department of Biology, Technion – Israel Institute of Technology, Haifa, Israel

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