We are analyzing https://link.springer.com/article/10.1186/s13059-015-0784-0.

Title:
WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system | Genome Biology
Description:
The CRISPR/Cas9 system has been rapidly adopted for genome editing. However, one major issue with this system is the lack of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy and specificity of genome editing. To address this pressing need, we analyze CRISPR RNA-seq data and identify many novel features that are characteristic of highly potent sgRNAs. These features are used to develop a bioinformatics tool for genome-wide design of sgRNAs with improved efficiency. These sgRNAs as well as the design tool are freely accessible via a web server, WU-CRISPR ( http://crispr.wustl.edu ).
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Technology & Computing
Science

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don't see any clear sign of profit-making.

Earning money isn't the goal of every website; some are designed to offer support or promote social causes. People have different reasons for creating websites. This might be one such reason. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

pubmed, sequence, cas, article, sgrnas, functional, genome, sgrna, google, scholar, guide, design, crispr, crisprcas, editing, grna, grnas, rna, features, central, analysis, nonfunctional, data, wucrispr, structural, study, system, target, activities, specificity, experimental, structure, end, svm, model, compared, nucleotides, fig, nat, rnas, significant, crossvalidation, chari, sequences, algorithm, prediction, accessibility, positions, bioinformatics, efficacy,

Topics {✒️}

support vector machine org/rnai/public/analysis-tools/sgrna-design weijun liu & xiaowei wang crispr-cas9-mediated gene inactivation crispr-cas9 genome-editing systems position-specific nucleotide accessibilities rna-programmed genome editing extended stem-loop structure position-specific nucleotide accessibility trans-encoded small rna stable rna/dna duplexes small-scale genomic alterations rna-guided cas9 nucleases rna-guided crispr cas9 cas9-based genome editing crispr/cas systems article download pdf natural crispr/cas9 process natural crispr/cas9 system cas protein complex gene-based cross-validation crispr-cas nucleases supplementary tables s1–s3 crispr-cas system pam-proximal seed region functional rna–protein complex tsai sq providing cross-validation data free energy calculation tenfold cross-validation results horizontal gene transfer pre-screened training data trans-activating crrna adaptive bacterial immunity related subjects genome-wide profiling ncbi gene ids crispr endonuclease cas9 grna/target sequence duplex full access precision-recall curve analysis privacy choices/manage cookies long noncoding rnas sequence motifs related short repeats interspaced creative commons license crispr-encoded rnas article wong inefficient genome editing efficient genome editing

Schema {🗺️}

WebPage:
      mainEntity:
         headline:WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
         description:The CRISPR/Cas9 system has been rapidly adopted for genome editing. However, one major issue with this system is the lack of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy and specificity of genome editing. To address this pressing need, we analyze CRISPR RNA-seq data and identify many novel features that are characteristic of highly potent sgRNAs. These features are used to develop a bioinformatics tool for genome-wide design of sgRNAs with improved efficiency. These sgRNAs as well as the design tool are freely accessible via a web server, WU-CRISPR ( http://crispr.wustl.edu ).
         datePublished:2015-11-02T00:00:00Z
         dateModified:2015-11-02T00:00:00Z
         pageStart:1
         pageEnd:8
         license:http://creativecommons.org/publicdomain/zero/1.0/
         sameAs:https://doi.org/10.1186/s13059-015-0784-0
         keywords:
            Support Vector Machine
            Support Vector Machine Model
            Seed Region
            Genome Editing
            Guide Sequence
            Animal Genetics and Genomics
            Human Genetics
            Plant Genetics and Genomics
            Microbial Genetics and Genomics
            Bioinformatics
            Evolutionary Biology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13059-015-0784-0/MediaObjects/13059_2015_784_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13059-015-0784-0/MediaObjects/13059_2015_784_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13059-015-0784-0/MediaObjects/13059_2015_784_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13059-015-0784-0/MediaObjects/13059_2015_784_Fig4_HTML.gif
         isPartOf:
            name:Genome Biology
            issn:
               1474-760X
            volumeNumber:16
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Nathan Wong
               affiliation:
                     name:Washington University
                     address:
                        name:Department of Biomedical Engineering, Washington University, St Louis, USA
                        type:PostalAddress
                     type:Organization
                     name:Washington University School of Medicine
                     address:
                        name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Weijun Liu
               affiliation:
                     name:Washington University School of Medicine
                     address:
                        name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xiaowei Wang
               affiliation:
                     name:Washington University School of Medicine
                     address:
                        name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
                        type:PostalAddress
                     type:Organization
                     name:Washington University
                     address:
                        name:Department of Biomedical Engineering, Washington University, St Louis, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
      description:The CRISPR/Cas9 system has been rapidly adopted for genome editing. However, one major issue with this system is the lack of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy and specificity of genome editing. To address this pressing need, we analyze CRISPR RNA-seq data and identify many novel features that are characteristic of highly potent sgRNAs. These features are used to develop a bioinformatics tool for genome-wide design of sgRNAs with improved efficiency. These sgRNAs as well as the design tool are freely accessible via a web server, WU-CRISPR ( http://crispr.wustl.edu ).
      datePublished:2015-11-02T00:00:00Z
      dateModified:2015-11-02T00:00:00Z
      pageStart:1
      pageEnd:8
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s13059-015-0784-0
      keywords:
         Support Vector Machine
         Support Vector Machine Model
         Seed Region
         Genome Editing
         Guide Sequence
         Animal Genetics and Genomics
         Human Genetics
         Plant Genetics and Genomics
         Microbial Genetics and Genomics
         Bioinformatics
         Evolutionary Biology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13059-015-0784-0/MediaObjects/13059_2015_784_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13059-015-0784-0/MediaObjects/13059_2015_784_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13059-015-0784-0/MediaObjects/13059_2015_784_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13059-015-0784-0/MediaObjects/13059_2015_784_Fig4_HTML.gif
      isPartOf:
         name:Genome Biology
         issn:
            1474-760X
         volumeNumber:16
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Nathan Wong
            affiliation:
                  name:Washington University
                  address:
                     name:Department of Biomedical Engineering, Washington University, St Louis, USA
                     type:PostalAddress
                  type:Organization
                  name:Washington University School of Medicine
                  address:
                     name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Weijun Liu
            affiliation:
                  name:Washington University School of Medicine
                  address:
                     name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xiaowei Wang
            affiliation:
                  name:Washington University School of Medicine
                  address:
                     name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
                     type:PostalAddress
                  type:Organization
                  name:Washington University
                  address:
                     name:Department of Biomedical Engineering, Washington University, St Louis, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Genome Biology
      issn:
         1474-760X
      volumeNumber:16
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Washington University
      address:
         name:Department of Biomedical Engineering, Washington University, St Louis, USA
         type:PostalAddress
      name:Washington University School of Medicine
      address:
         name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
         type:PostalAddress
      name:Washington University School of Medicine
      address:
         name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
         type:PostalAddress
      name:Washington University School of Medicine
      address:
         name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
         type:PostalAddress
      name:Washington University
      address:
         name:Department of Biomedical Engineering, Washington University, St Louis, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Nathan Wong
      affiliation:
            name:Washington University
            address:
               name:Department of Biomedical Engineering, Washington University, St Louis, USA
               type:PostalAddress
            type:Organization
            name:Washington University School of Medicine
            address:
               name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
               type:PostalAddress
            type:Organization
      name:Weijun Liu
      affiliation:
            name:Washington University School of Medicine
            address:
               name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
               type:PostalAddress
            type:Organization
      name:Xiaowei Wang
      affiliation:
            name:Washington University School of Medicine
            address:
               name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
               type:PostalAddress
            type:Organization
            name:Washington University
            address:
               name:Department of Biomedical Engineering, Washington University, St Louis, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Biomedical Engineering, Washington University, St Louis, USA
      name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
      name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
      name:Department of Radiation Oncology, Washington University School of Medicine, St Louis, USA
      name:Department of Biomedical Engineering, Washington University, St Louis, USA

External Links {🔗}(169)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.54s.

LINK . SPRINGER . COM {}