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  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
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We are analyzing https://link.springer.com/article/10.1186/s13046-018-0918-9.

Title:
Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway | Journal of Experimental & Clinical Cancer Research
Description:
Background Actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1), a long noncoding RNA, is significantly highly expressed and associated with metastasis and poor prognosis in many cancers, including nasopharyngeal carcinoma (NPC). In this study, we aim to identify the role of AFAP1-AS1 acting as an oncogenic lncRNA to promote NPC metastasis. Methods The role of AFAP1-AS1, miR-423-5p, and FOSL2 in NPC metastasis was investigated in vitro and in vivo. Bioinformatics analysis and luciferase activity assays were used to identify the interaction between AFAP1-AS1, miR-423-5p, and FOSL2. Additionally, real-time PCR and western blotting were used to assess the function of AFAP1-AS1 acting as an oncogenic lncRNA to promote NPC progression by regulating miR-423-5p and the downstream Rho/Rac pathway. Results In this study, we determined that AFAP1-AS1 functions as a competing endogenous RNA in NPC to regulate the Rho/Rac pathway through miR-423-5p. These interactions can mediate the expression of RAB11B, LASP1, and FOSL2 and accelerate cell migration and invasion via the Rho/Rac signaling pathway or FOSL2. AFAP1-AS1 and FOSL2 could competitively bind with miR-423-5p to regulate several molecules, including RAB11B and LASP1 of the Rho/Rac signaling pathway. AFAP1-AS1 can also regulate the expression of LASP1, which was transcriptionally regulated by FOSL2, resulting in increased migration and invasion of NPC cells via the Rho/Rac signaling pathway. Conclusions The observations in this study identify an important role for AFAP1-AS1 as a competing endogenous RNA (ceRNA) in NPC pathogenesis and indicate that it may serve as a potential target for cancer diagnosis and treatment.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {๐Ÿ“š}

  • Education
  • Science
  • Photography

Content Management System {๐Ÿ“}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {๐Ÿ“ˆ}

What is the average monthly size of link.springer.com audience?

๐ŸŒ  Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 7,734,772 visitors per month in the current month.

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How Does Link.springer.com Make Money? {๐Ÿ’ธ}

We don't see any clear sign of profit-making.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {๐Ÿ”}

mirp, afapas, expression, npc, fosl, article, cells, google, scholar, cancer, cell, fig, hne, nasopharyngeal, lasp, carcinoma, cas, rna, wang, luciferase, rhorac, zhang, metastasis, xiong, pathway, invasion, noncoding, rabb, signaling, binding, yang, zhou, activity, mimics, rac, migration, cerna, overexpression, long, zeng, control, analysis, tissues, transfection, regulate, gong, wei, tang, assays, vector,

Topics {โœ’๏ธ}

epstein-barr virus-encoded micrornas mtor-p-pi3k/akt-c-jun regulating mir-497-5p/celf1 pathway tgf-ฮฒ signaling pathway il-6/stat3 regulatory loop related rho/rock signaling rho/rac signaling pathway mirna real-time pcr cyclin d1/e2f pathways wild-type afap1-as1 reporter pmir-reporter-afap1-as1 vector cross-contaminated cell lines mutant ap1-binding sites downstream rho/rac pathway microrna-423-5p promotes autophagy articleย  google scholar nf-kappab pathway interplay kaplan-meier curve analysis rho/rac1 signaling pathway cgmp-dependent signaling protein effective metastasis-targeted therapy mir-423-5p seed region mir-423-5p directly targeted real-time pcr showed mir-423-5p binding sites article download pdf ap-1 consensus-binding site coding rna afap1-as1 prl-tk control vector regulating pten-dependent pathways entire afap1-as1 sequence 200ย ฮผl serum-free media chromosome 3p21 linked seed mir-423-5p sequence competitively binding mir-423-5p overexpressing afap1-as1 vector mir-423-5p mimics decreased afap1-as1 dramatically increased specific mir-423-5p mimics protein-coding gene expression synthetic mir-423-5p mimics gtpase family members mutant afap1-as1 sequence pmir-report luciferase vector mir-423-5p mimics compared poor relapse-free survival mir-423-5p seed sequences mirna response elements rho/rac signaling rho/rac signaling [44]

Questions {โ“}

  • A ceRNA hypothesis: the Rosetta stone of a hidden RNA language?
  • Rediscovery of NF-kappaB signaling in nasopharyngeal carcinoma: how genetic defects of NF-kappaB pathway interplay with EBV in driving oncogenesis?

Schema {๐Ÿ—บ๏ธ}

WebPage:
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         headline:Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway
         description:Actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1), a long noncoding RNA, is significantly highly expressed and associated with metastasis and poor prognosis in many cancers, including nasopharyngeal carcinoma (NPC). In this study, we aim to identify the role of AFAP1-AS1 acting as an oncogenic lncRNA to promote NPC metastasis. The role of AFAP1-AS1, miR-423-5p, and FOSL2 in NPC metastasis was investigated in vitro and in vivo. Bioinformatics analysis and luciferase activity assays were used to identify the interaction between AFAP1-AS1, miR-423-5p, and FOSL2. Additionally, real-time PCR and western blotting were used to assess the function of AFAP1-AS1 acting as an oncogenic lncRNA to promote NPC progression by regulating miR-423-5p and the downstream Rho/Rac pathway. In this study, we determined that AFAP1-AS1 functions as a competing endogenous RNA in NPC to regulate the Rho/Rac pathway through miR-423-5p. These interactions can mediate the expression of RAB11B, LASP1, and FOSL2 and accelerate cell migration and invasion via the Rho/Rac signaling pathway or FOSL2. AFAP1-AS1 and FOSL2 could competitively bind with miR-423-5p to regulate several molecules, including RAB11B and LASP1 of the Rho/Rac signaling pathway. AFAP1-AS1 can also regulate the expression of LASP1, which was transcriptionally regulated by FOSL2, resulting in increased migration and invasion of NPC cells via the Rho/Rac signaling pathway. The observations in this study identify an important role for AFAP1-AS1 as a competing endogenous RNA (ceRNA) in NPC pathogenesis and indicate that it may serve as a potential target for cancer diagnosis and treatment.
         datePublished:2018-10-16T00:00:00Z
         dateModified:2018-10-16T00:00:00Z
         pageStart:1
         pageEnd:17
         license:http://creativecommons.org/publicdomain/zero/1.0/
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         keywords:
            Long noncoding RNA (lncRNA)
             AFAP1-AS1
            miR-423-5p nasopharyngeal carcinoma (NPC)
            Competing endogenous RNA (ceRNA)
            Cancer Research
            Immunology
            Apoptosis
            Oncology
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                        name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
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ScholarlyArticle:
      headline:Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway
      description:Actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1), a long noncoding RNA, is significantly highly expressed and associated with metastasis and poor prognosis in many cancers, including nasopharyngeal carcinoma (NPC). In this study, we aim to identify the role of AFAP1-AS1 acting as an oncogenic lncRNA to promote NPC metastasis. The role of AFAP1-AS1, miR-423-5p, and FOSL2 in NPC metastasis was investigated in vitro and in vivo. Bioinformatics analysis and luciferase activity assays were used to identify the interaction between AFAP1-AS1, miR-423-5p, and FOSL2. Additionally, real-time PCR and western blotting were used to assess the function of AFAP1-AS1 acting as an oncogenic lncRNA to promote NPC progression by regulating miR-423-5p and the downstream Rho/Rac pathway. In this study, we determined that AFAP1-AS1 functions as a competing endogenous RNA in NPC to regulate the Rho/Rac pathway through miR-423-5p. These interactions can mediate the expression of RAB11B, LASP1, and FOSL2 and accelerate cell migration and invasion via the Rho/Rac signaling pathway or FOSL2. AFAP1-AS1 and FOSL2 could competitively bind with miR-423-5p to regulate several molecules, including RAB11B and LASP1 of the Rho/Rac signaling pathway. AFAP1-AS1 can also regulate the expression of LASP1, which was transcriptionally regulated by FOSL2, resulting in increased migration and invasion of NPC cells via the Rho/Rac signaling pathway. The observations in this study identify an important role for AFAP1-AS1 as a competing endogenous RNA (ceRNA) in NPC pathogenesis and indicate that it may serve as a potential target for cancer diagnosis and treatment.
      datePublished:2018-10-16T00:00:00Z
      dateModified:2018-10-16T00:00:00Z
      pageStart:1
      pageEnd:17
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s13046-018-0918-9
      keywords:
         Long noncoding RNA (lncRNA)
          AFAP1-AS1
         miR-423-5p nasopharyngeal carcinoma (NPC)
         Competing endogenous RNA (ceRNA)
         Cancer Research
         Immunology
         Apoptosis
         Oncology
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         name:Journal of Experimental & Clinical Cancer Research
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         name:BioMed Central
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            type:ImageObject
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      author:
            name:Yu Lian
            affiliation:
                  name:Xiangya Hospital, Central South University
                  address:
                     name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
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                  type:Organization
                  name:Ganzhou Hospital Affiliated to Nanchang University
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            name:Ganzhou Hospital Affiliated to Nanchang University
            address:
               name:Department of Reproductive medicine, Ganzhou Hospital Affiliated to Nanchang University, NanChang, China
               type:PostalAddress
            type:Organization
            name:University of North Dakota
            address:
               name:Department of Chemistry, University of North Dakota, Grand Forks, USA
               type:PostalAddress
            type:Organization
      name:Yong Li
      affiliation:
            name:Ganzhou Hospital Affiliated to Nanchang University
            address:
               name:Department of Reproductive medicine, Ganzhou Hospital Affiliated to Nanchang University, NanChang, China
               type:PostalAddress
            type:Organization
            name:Lerner Research Institute
            address:
               name:Department of Cancer Biology, Cleveland Clinic, Lerner Research Institute, Cleveland, USA
               type:PostalAddress
            type:Organization
      name:Xiaoling Li
      affiliation:
            name:Xiangya Hospital, Central South University
            address:
               name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
            name:Central South University
            address:
               name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
      name:Xiang Chen
      affiliation:
            name:Xiangya Hospital, Central South University
            address:
               name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
      name:Guiyuan Li
      affiliation:
            name:Xiangya Hospital, Central South University
            address:
               name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
            name:Central South University
            address:
               name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
      name:Can Guo
      affiliation:
            name:Xiangya Hospital, Central South University
            address:
               name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
            name:Central South University
            address:
               name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
      name:Zhaoyang Zeng
      affiliation:
            name:Xiangya Hospital, Central South University
            address:
               name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
            name:Central South University
            address:
               name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Wei Xiong
      affiliation:
            name:Xiangya Hospital, Central South University
            address:
               name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
            name:Central South University
            address:
               name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:Department of Reproductive medicine, Ganzhou Hospital Affiliated to Nanchang University, NanChang, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:Department of Reproductive medicine, Ganzhou Hospital Affiliated to Nanchang University, NanChang, China
      name:Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China
      name:Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, the Third Xiangya Hospital, Central South University, Changsha, China
      name:Department of Reproductive medicine, Ganzhou Hospital Affiliated to Nanchang University, NanChang, China
      name:Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China
      name:Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:Department of Reproductive medicine, Ganzhou Hospital Affiliated to Nanchang University, NanChang, China
      name:Department of Chemistry, University of North Dakota, Grand Forks, USA
      name:Department of Reproductive medicine, Ganzhou Hospital Affiliated to Nanchang University, NanChang, China
      name:Department of Cancer Biology, Cleveland Clinic, Lerner Research Institute, Cleveland, USA
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China
      name:The Key Laboratory of Carcinogenesis and OCancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, China

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