We are analyzing https://link.springer.com/article/10.1186/s13045-017-0408-0.

Title:
Tumor-recruited M2 macrophages promote gastric and breast cancer metastasis via M2 macrophage-secreted CHI3L1 protein | Journal of Hematology & Oncology
Description:
Background The macrophage, one of the several key immune cell types, is believed to be involved in tumorigenesis. However, the mechanism of macrophages promoting tumor progression is largely unknown. Methods The differentially secreted proteins of M1 and M2 macrophages were analyzed by mass spectrometry. We performed GST pull-down assay for the identification of cell-membrane receptors that interact with chitinase 3-like protein 1 (CHI3L1) protein. The mouse model was used to validate the function of CHI3L1 in cancer metastasis in vivo. Protein phosphorylation and gene expression were performed to study the signaling pathway activation of cancer cells after CHI3L1 treatment. Results M2 macrophage-secreted CHI3L1 promoted the metastasis of gastric and breast cancer cells in vitro and in vivo. The CHI3L1 protein functioned by interacting with interleukin-13 receptor α2 chain (IL-13Rα2) molecules on the plasma membranes of cancer cells. Activation of IL-13Rα2 by CHI3L1 triggered the activation of the mitogen-activated protein kinase signaling pathway, leading to the upregulated expression of matrix metalloproteinase genes, which promoted tumor metastasis. The results of this study indicated that the level of CHI3L1 protein in the sera of patients with gastric or breast cancer was significantly elevated compared with those of healthy donors. Conclusions Our study revealed a novel aspect of macrophages with respect to cancer metastasis and showed that CHI3L1 could be a marker of metastatic gastric and breast cancer in patients.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Health & Fitness
Science
Education

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,016 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't see how the site brings in money.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Link.springer.com might be earning cash quietly, but we haven't detected the monetization method.

Keywords {🔍}

cancer, cells, chil, cell, macrophages, protein, breast, metastasis, gastric, mdamb, fig, article, pubmed, tumor, expression, google, scholar, cas, migration, invasion, ilrα, proteins, data, levels, isolated, results, adhesion, mice, mkn, showed, gene, signaling, patients, role, scalebar, ags, central, pathway, significantly, blood, antichil, macrophage, culture, rchil, promote, study, compared, recombinant, cocultured, assays,

Topics {✒️}

tgf-β-induced epithelial-mesenchymal transition ras/raf-1/erk/ap-1 signaling pathway granulocyte-macrophage colony-stimulating factor c-jun n-terminal kinase erk-nf-κb signaling full size image selective jnk/ap-1/mmp-2 modulation article download pdf m2 macrophage-derived chi3l1 chi3l1-mediated il-13rα2 activation mitogen-activated protein kinase exclusively tam-derived cytokines suppress t-cell proliferation promote solid-tumor metastasis dual-luciferase reporter assays quantitative real-time pcr erα-negative breast cancer pro-inflammatory cytokines derived human leukocyte antigen-drα t-cell stimulatory cytokines peripheral blood monocytes mediate cell-cell interactions m2-secreted oncogenic protein il-13rα2 receptor activated perform anti-tumor functions il-13rα2-sirna transfection western blotting-based determination il-13rα2 gene silencing il-13rα2 gene expression tumor-derived signals macrophage-mediated mechanism chi3l1-ap-1-mmp pathway sds-page denaturing gel polyclonal anti-chi3l1 antibody mda-mb-435 cell metastasis mda-mb-231 cell metastasis myeloid wnt7b mediates m2 macrophage secretions pi3k/akt signaling pathways c-jun protein expression fluorescently labeled anti-chi3l1 m2 macrophages derived full access chi3l1-binding membrane receptor c-jun expression levels published article chi3l1-il-13rα2 interaction colony-stimulating factor-1 hla-drα-positive macrophages monocyte-derived m1

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Tumor-recruited M2 macrophages promote gastric and breast cancer metastasis via M2 macrophage-secreted CHI3L1 protein
         description:The macrophage, one of the several key immune cell types, is believed to be involved in tumorigenesis. However, the mechanism of macrophages promoting tumor progression is largely unknown. The differentially secreted proteins of M1 and M2 macrophages were analyzed by mass spectrometry. We performed GST pull-down assay for the identification of cell-membrane receptors that interact with chitinase 3-like protein 1 (CHI3L1) protein. The mouse model was used to validate the function of CHI3L1 in cancer metastasis in vivo. Protein phosphorylation and gene expression were performed to study the signaling pathway activation of cancer cells after CHI3L1 treatment. M2 macrophage-secreted CHI3L1 promoted the metastasis of gastric and breast cancer cells in vitro and in vivo. The CHI3L1 protein functioned by interacting with interleukin-13 receptor α2 chain (IL-13Rα2) molecules on the plasma membranes of cancer cells. Activation of IL-13Rα2 by CHI3L1 triggered the activation of the mitogen-activated protein kinase signaling pathway, leading to the upregulated expression of matrix metalloproteinase genes, which promoted tumor metastasis. The results of this study indicated that the level of CHI3L1 protein in the sera of patients with gastric or breast cancer was significantly elevated compared with those of healthy donors. Our study revealed a novel aspect of macrophages with respect to cancer metastasis and showed that CHI3L1 could be a marker of metastatic gastric and breast cancer in patients.
         datePublished:2017-02-01T00:00:00Z
         dateModified:2017-02-01T00:00:00Z
         pageStart:1
         pageEnd:13
         license:http://creativecommons.org/publicdomain/zero/1.0/
         sameAs:https://doi.org/10.1186/s13045-017-0408-0
         keywords:
            AP-1
            Cancer metastasis
            CHI3L1
            IL-13Rα2
            M2 macrophage
            Oncology
            Hematology
            Cancer Research
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig6_HTML.gif
         isPartOf:
            name:Journal of Hematology & Oncology
            issn:
               1756-8722
            volumeNumber:10
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Yulei Chen
               affiliation:
                     name:Zhejiang University
                     address:
                        name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Siyuan Zhang
               affiliation:
                     name:Zhejiang University
                     address:
                        name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Qizhi Wang
               affiliation:
                     name:The First Affiliated Hospital of Bengbu Medical College
                     address:
                        name:Department of Gastroenterology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xiaobo Zhang
               affiliation:
                     name:Zhejiang University
                     address:
                        name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Tumor-recruited M2 macrophages promote gastric and breast cancer metastasis via M2 macrophage-secreted CHI3L1 protein
      description:The macrophage, one of the several key immune cell types, is believed to be involved in tumorigenesis. However, the mechanism of macrophages promoting tumor progression is largely unknown. The differentially secreted proteins of M1 and M2 macrophages were analyzed by mass spectrometry. We performed GST pull-down assay for the identification of cell-membrane receptors that interact with chitinase 3-like protein 1 (CHI3L1) protein. The mouse model was used to validate the function of CHI3L1 in cancer metastasis in vivo. Protein phosphorylation and gene expression were performed to study the signaling pathway activation of cancer cells after CHI3L1 treatment. M2 macrophage-secreted CHI3L1 promoted the metastasis of gastric and breast cancer cells in vitro and in vivo. The CHI3L1 protein functioned by interacting with interleukin-13 receptor α2 chain (IL-13Rα2) molecules on the plasma membranes of cancer cells. Activation of IL-13Rα2 by CHI3L1 triggered the activation of the mitogen-activated protein kinase signaling pathway, leading to the upregulated expression of matrix metalloproteinase genes, which promoted tumor metastasis. The results of this study indicated that the level of CHI3L1 protein in the sera of patients with gastric or breast cancer was significantly elevated compared with those of healthy donors. Our study revealed a novel aspect of macrophages with respect to cancer metastasis and showed that CHI3L1 could be a marker of metastatic gastric and breast cancer in patients.
      datePublished:2017-02-01T00:00:00Z
      dateModified:2017-02-01T00:00:00Z
      pageStart:1
      pageEnd:13
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s13045-017-0408-0
      keywords:
         AP-1
         Cancer metastasis
         CHI3L1
         IL-13Rα2
         M2 macrophage
         Oncology
         Hematology
         Cancer Research
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13045-017-0408-0/MediaObjects/13045_2017_408_Fig6_HTML.gif
      isPartOf:
         name:Journal of Hematology & Oncology
         issn:
            1756-8722
         volumeNumber:10
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Yulei Chen
            affiliation:
                  name:Zhejiang University
                  address:
                     name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Siyuan Zhang
            affiliation:
                  name:Zhejiang University
                  address:
                     name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Qizhi Wang
            affiliation:
                  name:The First Affiliated Hospital of Bengbu Medical College
                  address:
                     name:Department of Gastroenterology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xiaobo Zhang
            affiliation:
                  name:Zhejiang University
                  address:
                     name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Journal of Hematology & Oncology
      issn:
         1756-8722
      volumeNumber:10
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Zhejiang University
      address:
         name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
         type:PostalAddress
      name:Zhejiang University
      address:
         name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
         type:PostalAddress
      name:The First Affiliated Hospital of Bengbu Medical College
      address:
         name:Department of Gastroenterology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, People’s Republic of China
         type:PostalAddress
      name:Zhejiang University
      address:
         name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Yulei Chen
      affiliation:
            name:Zhejiang University
            address:
               name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Siyuan Zhang
      affiliation:
            name:Zhejiang University
            address:
               name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Qizhi Wang
      affiliation:
            name:The First Affiliated Hospital of Bengbu Medical College
            address:
               name:Department of Gastroenterology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Xiaobo Zhang
      affiliation:
            name:Zhejiang University
            address:
               name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
      name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China
      name:Department of Gastroenterology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, People’s Republic of China
      name:College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People’s Republic of China

External Links {🔗}(161)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.81s.

LINK . SPRINGER . COM {}