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Title:
Antitumor effect of IL-12 gene-modified bone marrow mesenchymal stem cells combined with Fuzheng Yiliu decoction in an in vivo glioma nude mouse model | Journal of Translational Medicine
Description:
Background Glioma is a complex cancer with a high morbidity and high mortality. Bone marrow mesenchymal stem cells (BMSCs) have shown promise as an excellent cell/drug delivery vehicle for gene-targeted therapy; however, maintaining genetic stability and biological activity remains difficult. Furthermore, whether BMSCs support or inhibit tumor growth remains debated. This study investigated whether a traditional Chinese medicine fomular, Fuzheng Yiliu decoction (FYD) had a synergistic antitumor effect with IL-12 gene-modified BMSCs in glioma-bearing nude mice Methods The lentivirus-mediated IL-12 gene was transfected into primarily cultured BMSCs. A total of 72 BALB/c nude mice were used to establish xenograft models with glioma U251 cells and were divided into groups (n = 12) including blank control group, nude mouse model group (model group), lentiviral transfection of BMSC group with no gene loading (BMSC group), IL-12 lentivirus-transfected BMSC group (IL-12 + BMSC group), FYD treatment group (FYD group), and FYD treatment in IL-12 lentivirus-transfected BMSC group (FYD + IL-12 + BMSC group).. After treatment for 14 days, all mice were sacrificed to collect tumor tissue and serum for more detection, such as distribution of BMSCs, cell apoptosis in xenograft tumors, serum IL-12 and INF-γ levels, mouse weight and tumor volume were measured Results There were significantly more apoptotic cells in tumor tissue in IL-12 gene transfected group, FYD treatment group and FYD combining with IL-12 gene transfected group than that in the model group (P < 0.05). The FYD + IL-12 + BMSC group showed significantly higher Bax and lower Bcl-2 expression (P < 0.05), and serum IL-12 and INF-γ levels (P < 0.05) were higher than that in all other groups. After the intervention, this group also showed a strong inhibitory effect against tumor growth (P < 0.05) Conclusions This study suggested FYD treatment combined with IL-12 gene-modified BMSCs shows synergistic antitumor effect in glioma-bearing nude mice.
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Keywords {🔍}
group, bmsc, cells, fyd, bmscs, tumor, groups, stem, mice, google, scholar, mesenchymal, article, pubmed, model, treatment, bone, expression, gene, cell, marrow, effect, volume, chinese, medicine, cas, levels, gansu, glioma, cancer, growth, study, staining, tumors, compared, antitumor, decoction, apoptosis, serum, weight, day, fig, nude, significantly, significant, therapy, higher, human, tissues, fluorescence,
Topics {✒️}
stromal cell-derived factor-1alpha human il-12/ifn-gamma elisa high-purity endotoxin-free kit long-term outcomes post-treatment mesenchymal stem cells human glioma stem il12-lentiviral-transfected bmsc dark-brown nuclear staining fyd + il-12 + bmsc group exhibited lentivirus-mediated il-12 gene fyd + il-12 + bmsc group showed spindle-shaped cells appeared fyd + il-12 + bmsc group compared glioma stem cells immune-mediated gene therapy spindle-shaped morphologies appeared altered bax/bcl-2-p53 expression mapkk-dependent cross-talk horseradish peroxidase-labeled streptavidin article download pdf fyd + il-12 + bmsc groups compared glioma u251 cells drug/gene targeted carriers serum-deprived k562 cells enzyme-linked immunosorbent assay full access il-12 gene-modified bmscs bone marrow il-12 + bmsc group exhibited glioma-bearing nude mice inf gene-modified bmscs fuzheng yiliu decoction fuzhengyiliu granule contained-serum fuzheng yiliu fang u251-mg cells fyd + il-12 + bmsc group specific pathogen-free balb/ human malignant glioma anti-tumor chinese materiamedica fuzhengyiliu decoction il-12 + bmsc 350 mg/kg injected intraperitoneally human gene therapy related subjects tumor-bearing nude mice explore relevant mechanisms exhibited brown particles cell-based therapies fyd + il-12 + bmsc groups prostate cancer cells apoptotic cells demonstrating
Questions {❓}
- Stepanenko AA, Chekhonin VP: Recent Advances in Oncolytic Virotherapy and Immunotherapy for Glioblastoma: A Glimmer of Hope in the Search for an Effective Therapy?
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headline:Antitumor effect of IL-12 gene-modified bone marrow mesenchymal stem cells combined with Fuzheng Yiliu decoction in an in vivo glioma nude mouse model
description:Glioma is a complex cancer with a high morbidity and high mortality. Bone marrow mesenchymal stem cells (BMSCs) have shown promise as an excellent cell/drug delivery vehicle for gene-targeted therapy; however, maintaining genetic stability and biological activity remains difficult. Furthermore, whether BMSCs support or inhibit tumor growth remains debated. This study investigated whether a traditional Chinese medicine fomular, Fuzheng Yiliu decoction (FYD) had a synergistic antitumor effect with IL-12 gene-modified BMSCs in glioma-bearing nude mice The lentivirus-mediated IL-12 gene was transfected into primarily cultured BMSCs. A total of 72 BALB/c nude mice were used to establish xenograft models with glioma U251 cells and were divided into groups (n = 12) including blank control group, nude mouse model group (model group), lentiviral transfection of BMSC group with no gene loading (BMSC group), IL-12 lentivirus-transfected BMSC group (IL-12 + BMSC group), FYD treatment group (FYD group), and FYD treatment in IL-12 lentivirus-transfected BMSC group (FYD + IL-12 + BMSC group).. After treatment for 14 days, all mice were sacrificed to collect tumor tissue and serum for more detection, such as distribution of BMSCs, cell apoptosis in xenograft tumors, serum IL-12 and INF-γ levels, mouse weight and tumor volume were measured There were significantly more apoptotic cells in tumor tissue in IL-12 gene transfected group, FYD treatment group and FYD combining with IL-12 gene transfected group than that in the model group (P < 0.05). The FYD + IL-12 + BMSC group showed significantly higher Bax and lower Bcl-2 expression (P < 0.05), and serum IL-12 and INF-γ levels (P < 0.05) were higher than that in all other groups. After the intervention, this group also showed a strong inhibitory effect against tumor growth (P < 0.05) This study suggested FYD treatment combined with IL-12 gene-modified BMSCs shows synergistic antitumor effect in glioma-bearing nude mice.
datePublished:2021-04-07T00:00:00Z
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IL-12
Fuzheng Yiliu decoction
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description:Glioma is a complex cancer with a high morbidity and high mortality. Bone marrow mesenchymal stem cells (BMSCs) have shown promise as an excellent cell/drug delivery vehicle for gene-targeted therapy; however, maintaining genetic stability and biological activity remains difficult. Furthermore, whether BMSCs support or inhibit tumor growth remains debated. This study investigated whether a traditional Chinese medicine fomular, Fuzheng Yiliu decoction (FYD) had a synergistic antitumor effect with IL-12 gene-modified BMSCs in glioma-bearing nude mice The lentivirus-mediated IL-12 gene was transfected into primarily cultured BMSCs. A total of 72 BALB/c nude mice were used to establish xenograft models with glioma U251 cells and were divided into groups (n = 12) including blank control group, nude mouse model group (model group), lentiviral transfection of BMSC group with no gene loading (BMSC group), IL-12 lentivirus-transfected BMSC group (IL-12 + BMSC group), FYD treatment group (FYD group), and FYD treatment in IL-12 lentivirus-transfected BMSC group (FYD + IL-12 + BMSC group).. After treatment for 14 days, all mice were sacrificed to collect tumor tissue and serum for more detection, such as distribution of BMSCs, cell apoptosis in xenograft tumors, serum IL-12 and INF-γ levels, mouse weight and tumor volume were measured There were significantly more apoptotic cells in tumor tissue in IL-12 gene transfected group, FYD treatment group and FYD combining with IL-12 gene transfected group than that in the model group (P < 0.05). The FYD + IL-12 + BMSC group showed significantly higher Bax and lower Bcl-2 expression (P < 0.05), and serum IL-12 and INF-γ levels (P < 0.05) were higher than that in all other groups. After the intervention, this group also showed a strong inhibitory effect against tumor growth (P < 0.05) This study suggested FYD treatment combined with IL-12 gene-modified BMSCs shows synergistic antitumor effect in glioma-bearing nude mice.
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Glioma
IL-12
Fuzheng Yiliu decoction
Biomedicine
general
Medicine/Public Health
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affiliation:
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address:
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
type:PostalAddress
type:Organization
name:Gansu University of Chinese Medicine
address:
name:School of Integrated Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou, China
type:PostalAddress
type:Organization
name:Kai Liu
affiliation:
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level
address:
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
type:PostalAddress
type:Organization
name:Gansu University of Chinese Medicine
address:
name:School of Integrated Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou, China
type:PostalAddress
type:Organization
name:Yongqi Liu
affiliation:
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level
address:
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
type:PostalAddress
type:Organization
name:Gansu University of Chinese Medicine
address:
name:School of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou, China
type:PostalAddress
type:Organization
name:Rui Xu
affiliation:
name:Gansu University of Chinese Medicine
address:
name:School of Public Health, Gansu University of Chinese Medicine, Lanzhou, China
type:PostalAddress
type:Organization
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level
address:
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
type:PostalAddress
type:Organization
name:Guisen Zheng
affiliation:
name:Gansu University of Chinese Medicine
address:
name:School of Public Health, Gansu University of Chinese Medicine, Lanzhou, China
type:PostalAddress
type:Organization
name:Provincial Key Laboratory of Chinese Medicine Prevention and Control of Chronic Diseases
address:
name:Provincial Key Laboratory of Chinese Medicine Prevention and Control of Chronic Diseases, Lanzhou, China
type:PostalAddress
type:Organization
email:[email protected]
PostalAddress:
name:School of Public Health, Gansu University of Chinese Medicine, Lanzhou, China
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
name:Provincial Key Laboratory of Chinese Medicine Prevention and Control of Chronic Diseases, Lanzhou, China
name:Department of Neurology, The First People’s Hospital of Lanzhou City, Lanzhou, China
name:School of Public Health, Gansu University of Chinese Medicine, Lanzhou, China
name:Provincial Key Laboratory of Chinese Medicine Prevention and Control of Chronic Diseases, Lanzhou, China
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
name:Provincial Key Laboratory of Chinese Medicine Prevention and Control of Chronic Diseases, Lanzhou, China
name:School of Public Health, Gansu University of Chinese Medicine, Lanzhou, China
name:Provincial Key Laboratory of Chinese Medicine Prevention and Control of Chronic Diseases, Lanzhou, China
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
name:School of Integrated Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou, China
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
name:School of Integrated Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou, China
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
name:School of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou, China
name:School of Public Health, Gansu University of Chinese Medicine, Lanzhou, China
name:Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou, China
name:School of Public Health, Gansu University of Chinese Medicine, Lanzhou, China
name:Provincial Key Laboratory of Chinese Medicine Prevention and Control of Chronic Diseases, Lanzhou, China
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