Here's how LINK.SPRINGER.COM makes money* and how much!

*Please read our disclaimer before using our estimates.
Loading...

LINK . SPRINGER . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Schema
  9. External Links
  10. Analytics And Tracking
  11. Libraries
  12. CDN Services

We are analyzing https://link.springer.com/article/10.1186/s12885-019-5403-0.

Title:
Roadmap of DNA methylation in breast cancer identifies novel prognostic biomarkers | BMC Cancer
Description:
Background Breast cancer is a highly heterogeneous disease resulting in diverse clinical behaviours and therapeutic responses. DNA methylation is a major epigenetic alteration that is commonly perturbed in cancers. The aim of this study is to characterize the relationship between DNA methylation and aberrant gene expression in breast cancer. Methods We analysed DNA methylation and gene expression profiles from breast cancer tissue and matched normal tissue in The Cancer Genome Atlas (TCGA). Genome-wide differential methylation analysis and methylation-gene expression correlation was performed. Gene expression changes were subsequently validated in the METABRIC dataset. The Oncoscore tool was used to identify genes that had previously been associated with cancer in the literature. A subset of genes that had not previously been studied in cancer was chosen for further analysis. Results We identified 368 CpGs that were differentially methylated between tumor and normal breast tissue (∆β > 0.4). Hypermethylated CpGs were overrepresented in tumor tissue and were found predominantly (56%) in upstream promoter regions. Conversely, hypomethylated CpG sites were found primarily in the gene body (66%). Expression analysis revealed that 209 of the differentially-methylated CpGs were located in 169 genes that were differently expressed between normal and breast tumor tissue. Methylation-expression correlations were predominantly negative (70%) for promoter CpG sites and positive (74%) for gene body CpG sites. Among these differentially-methylated and differentially-expressed genes, we identified 7 that had not previously been studied in any form of cancer. Three of these, TDRD10, PRAC2 and TMEM132C, contained CpG sites that showed diagnostic and prognostic value in breast cancer, particularly in estrogen-receptor (ER)-positive samples. A pan-cancer analysis confirmed differential expression of these genes together with diagnostic and prognostic value of their respective CpG sites in multiple cancer types. Conclusion We have identified 368 DNA methylation changes that characterize breast cancer tumor tissue, of which 209 are associated with genes that are differentially-expressed in the same samples. Novel DNA methylation markers were identified, of which cg12374721 (PRAC2), cg18081940 (TDRD10) and cg04475027 (TMEM132C) show promise as diagnostic and prognostic markers in breast cancer as well as other cancer types.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Education
  • Science
  • Health & Fitness

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't figure out the monetization strategy.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com could be getting rich in stealth mode, or the way it's monetizing isn't detectable.

Keywords {🔍}

cancer, breast, methylation, genes, gene, cpg, sites, expression, dna, article, google, scholar, tumor, analysis, data, tissue, prognostic, cas, table, normal, prac, tmemc, fig, additional, tdrd, file, diagnostic, tcga, promoter, cpgs, analyses, hypermethylated, hkme, located, survival, change, metabric, hypomethylated, samples, potential, correlated, differentiallymethylated, chromatin, prognosis, transcription, hypermethylation, figure, oncoscore, previously, results,

Topics {✒️}

pgc-1α-responsive genes involved pedro castelo-branco pd/bd/105899/2014 fct fellowship related subjects grch37/hg19 genome assembly breast cancer heterogeneity methylated arginine/lysine residues genome-wide expression analyses performed genome-wide analyses dna methylation profiles article download pdf cancer-related article medip-seq data shows research article methylation kaplan-meir survival curves dna methylation-gene markers differentially-methylated cpg sites early-stage breast cancer cytosine-phosphate-guanine fdr kaplan-meier survival curves castelo-branco full access dna methylation-based diagnostic gene expression profiles epigenomic regulatory elements data access request cox multivariate analyses long-range epigenetic remodeling cancer-related deaths [3] genome-wide approach hierarchical clustering analyses dna methylation patterns low methylation levels methyl-binding domain proteins roadmap analysis revealed functional enrichment analysis distinct clinical behaviours privacy choices/manage cookies cancer genome atlas breast cancer identifies functional analysis revealed including dna methylation methylation-gene expression correlation unsupervised hierarchical clustering intragenic dna hypomethylation positively-correlated cpg sites joana dias apolónio matched-normal tcga samples negatively-correlated cpg sites dna methylation markers

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Roadmap of DNA methylation in breast cancer identifies novel prognostic biomarkers
         description:Breast cancer is a highly heterogeneous disease resulting in diverse clinical behaviours and therapeutic responses. DNA methylation is a major epigenetic alteration that is commonly perturbed in cancers. The aim of this study is to characterize the relationship between DNA methylation and aberrant gene expression in breast cancer. We analysed DNA methylation and gene expression profiles from breast cancer tissue and matched normal tissue in The Cancer Genome Atlas (TCGA). Genome-wide differential methylation analysis and methylation-gene expression correlation was performed. Gene expression changes were subsequently validated in the METABRIC dataset. The Oncoscore tool was used to identify genes that had previously been associated with cancer in the literature. A subset of genes that had not previously been studied in cancer was chosen for further analysis. We identified 368 CpGs that were differentially methylated between tumor and normal breast tissue (∆β > 0.4). Hypermethylated CpGs were overrepresented in tumor tissue and were found predominantly (56%) in upstream promoter regions. Conversely, hypomethylated CpG sites were found primarily in the gene body (66%). Expression analysis revealed that 209 of the differentially-methylated CpGs were located in 169 genes that were differently expressed between normal and breast tumor tissue. Methylation-expression correlations were predominantly negative (70%) for promoter CpG sites and positive (74%) for gene body CpG sites. Among these differentially-methylated and differentially-expressed genes, we identified 7 that had not previously been studied in any form of cancer. Three of these, TDRD10, PRAC2 and TMEM132C, contained CpG sites that showed diagnostic and prognostic value in breast cancer, particularly in estrogen-receptor (ER)-positive samples. A pan-cancer analysis confirmed differential expression of these genes together with diagnostic and prognostic value of their respective CpG sites in multiple cancer types. We have identified 368 DNA methylation changes that characterize breast cancer tumor tissue, of which 209 are associated with genes that are differentially-expressed in the same samples. Novel DNA methylation markers were identified, of which cg12374721 (PRAC2), cg18081940 (TDRD10) and cg04475027 (TMEM132C) show promise as diagnostic and prognostic markers in breast cancer as well as other cancer types.
         datePublished:2019-03-12T00:00:00Z
         dateModified:2019-03-12T00:00:00Z
         pageStart:1
         pageEnd:12
         license:http://creativecommons.org/publicdomain/zero/1.0/
         sameAs:https://doi.org/10.1186/s12885-019-5403-0
         keywords:
            Breast cancer
            DNA methylation
            Biomarkers
            Diagnostic
            Prognostic
            Cancer Research
            Oncology
            Surgical Oncology
            Health Promotion and Disease Prevention
            Biomedicine
            general
            Medicine/Public Health
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig4_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig5_HTML.png
         isPartOf:
            name:BMC Cancer
            issn:
               1471-2407
            volumeNumber:19
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Bernardo P. de Almeida
               affiliation:
                     name:University of Lisbon
                     address:
                        name:Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, Lisbon, Portugal
                        type:PostalAddress
                     type:Organization
                     name:University of Algarve
                     address:
                        name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
                        type:PostalAddress
                     type:Organization
                     name:Vienna Biocenter (VBC)
                     address:
                        name:Present address: Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Vienna, Austria
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Joana Dias Apolónio
               url:http://orcid.org/0000-0002-9727-1813
               affiliation:
                     name:University of Algarve
                     address:
                        name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
                        type:PostalAddress
                     type:Organization
                     name:University of Algarve
                     address:
                        name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
                        type:PostalAddress
                     type:Organization
                     name:Campus Gambelas
                     address:
                        name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Alexandra Binnie
               affiliation:
                     name:University of Algarve
                     address:
                        name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
                        type:PostalAddress
                     type:Organization
                     name:University of Algarve
                     address:
                        name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
                        type:PostalAddress
                     type:Organization
                     name:Campus Gambelas
                     address:
                        name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
                        type:PostalAddress
                     type:Organization
                     name:William Osler Health System
                     address:
                        name:William Osler Health System, Brampton, Canada
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Pedro Castelo-Branco
               affiliation:
                     name:University of Algarve
                     address:
                        name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
                        type:PostalAddress
                     type:Organization
                     name:University of Algarve
                     address:
                        name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
                        type:PostalAddress
                     type:Organization
                     name:Campus Gambelas
                     address:
                        name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Roadmap of DNA methylation in breast cancer identifies novel prognostic biomarkers
      description:Breast cancer is a highly heterogeneous disease resulting in diverse clinical behaviours and therapeutic responses. DNA methylation is a major epigenetic alteration that is commonly perturbed in cancers. The aim of this study is to characterize the relationship between DNA methylation and aberrant gene expression in breast cancer. We analysed DNA methylation and gene expression profiles from breast cancer tissue and matched normal tissue in The Cancer Genome Atlas (TCGA). Genome-wide differential methylation analysis and methylation-gene expression correlation was performed. Gene expression changes were subsequently validated in the METABRIC dataset. The Oncoscore tool was used to identify genes that had previously been associated with cancer in the literature. A subset of genes that had not previously been studied in cancer was chosen for further analysis. We identified 368 CpGs that were differentially methylated between tumor and normal breast tissue (∆β > 0.4). Hypermethylated CpGs were overrepresented in tumor tissue and were found predominantly (56%) in upstream promoter regions. Conversely, hypomethylated CpG sites were found primarily in the gene body (66%). Expression analysis revealed that 209 of the differentially-methylated CpGs were located in 169 genes that were differently expressed between normal and breast tumor tissue. Methylation-expression correlations were predominantly negative (70%) for promoter CpG sites and positive (74%) for gene body CpG sites. Among these differentially-methylated and differentially-expressed genes, we identified 7 that had not previously been studied in any form of cancer. Three of these, TDRD10, PRAC2 and TMEM132C, contained CpG sites that showed diagnostic and prognostic value in breast cancer, particularly in estrogen-receptor (ER)-positive samples. A pan-cancer analysis confirmed differential expression of these genes together with diagnostic and prognostic value of their respective CpG sites in multiple cancer types. We have identified 368 DNA methylation changes that characterize breast cancer tumor tissue, of which 209 are associated with genes that are differentially-expressed in the same samples. Novel DNA methylation markers were identified, of which cg12374721 (PRAC2), cg18081940 (TDRD10) and cg04475027 (TMEM132C) show promise as diagnostic and prognostic markers in breast cancer as well as other cancer types.
      datePublished:2019-03-12T00:00:00Z
      dateModified:2019-03-12T00:00:00Z
      pageStart:1
      pageEnd:12
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s12885-019-5403-0
      keywords:
         Breast cancer
         DNA methylation
         Biomarkers
         Diagnostic
         Prognostic
         Cancer Research
         Oncology
         Surgical Oncology
         Health Promotion and Disease Prevention
         Biomedicine
         general
         Medicine/Public Health
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig4_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-019-5403-0/MediaObjects/12885_2019_5403_Fig5_HTML.png
      isPartOf:
         name:BMC Cancer
         issn:
            1471-2407
         volumeNumber:19
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Bernardo P. de Almeida
            affiliation:
                  name:University of Lisbon
                  address:
                     name:Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, Lisbon, Portugal
                     type:PostalAddress
                  type:Organization
                  name:University of Algarve
                  address:
                     name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
                     type:PostalAddress
                  type:Organization
                  name:Vienna Biocenter (VBC)
                  address:
                     name:Present address: Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Vienna, Austria
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Joana Dias Apolónio
            url:http://orcid.org/0000-0002-9727-1813
            affiliation:
                  name:University of Algarve
                  address:
                     name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
                     type:PostalAddress
                  type:Organization
                  name:University of Algarve
                  address:
                     name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
                     type:PostalAddress
                  type:Organization
                  name:Campus Gambelas
                  address:
                     name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Alexandra Binnie
            affiliation:
                  name:University of Algarve
                  address:
                     name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
                     type:PostalAddress
                  type:Organization
                  name:University of Algarve
                  address:
                     name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
                     type:PostalAddress
                  type:Organization
                  name:Campus Gambelas
                  address:
                     name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
                     type:PostalAddress
                  type:Organization
                  name:William Osler Health System
                  address:
                     name:William Osler Health System, Brampton, Canada
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Pedro Castelo-Branco
            affiliation:
                  name:University of Algarve
                  address:
                     name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
                     type:PostalAddress
                  type:Organization
                  name:University of Algarve
                  address:
                     name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
                     type:PostalAddress
                  type:Organization
                  name:Campus Gambelas
                  address:
                     name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:BMC Cancer
      issn:
         1471-2407
      volumeNumber:19
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Lisbon
      address:
         name:Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, Lisbon, Portugal
         type:PostalAddress
      name:University of Algarve
      address:
         name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
         type:PostalAddress
      name:Vienna Biocenter (VBC)
      address:
         name:Present address: Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Vienna, Austria
         type:PostalAddress
      name:University of Algarve
      address:
         name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
         type:PostalAddress
      name:University of Algarve
      address:
         name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
         type:PostalAddress
      name:Campus Gambelas
      address:
         name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
         type:PostalAddress
      name:University of Algarve
      address:
         name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
         type:PostalAddress
      name:University of Algarve
      address:
         name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
         type:PostalAddress
      name:Campus Gambelas
      address:
         name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
         type:PostalAddress
      name:William Osler Health System
      address:
         name:William Osler Health System, Brampton, Canada
         type:PostalAddress
      name:University of Algarve
      address:
         name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
         type:PostalAddress
      name:University of Algarve
      address:
         name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
         type:PostalAddress
      name:Campus Gambelas
      address:
         name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Bernardo P. de Almeida
      affiliation:
            name:University of Lisbon
            address:
               name:Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, Lisbon, Portugal
               type:PostalAddress
            type:Organization
            name:University of Algarve
            address:
               name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
               type:PostalAddress
            type:Organization
            name:Vienna Biocenter (VBC)
            address:
               name:Present address: Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Vienna, Austria
               type:PostalAddress
            type:Organization
      name:Joana Dias Apolónio
      url:http://orcid.org/0000-0002-9727-1813
      affiliation:
            name:University of Algarve
            address:
               name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
               type:PostalAddress
            type:Organization
            name:University of Algarve
            address:
               name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
               type:PostalAddress
            type:Organization
            name:Campus Gambelas
            address:
               name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
               type:PostalAddress
            type:Organization
      name:Alexandra Binnie
      affiliation:
            name:University of Algarve
            address:
               name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
               type:PostalAddress
            type:Organization
            name:University of Algarve
            address:
               name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
               type:PostalAddress
            type:Organization
            name:Campus Gambelas
            address:
               name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
               type:PostalAddress
            type:Organization
            name:William Osler Health System
            address:
               name:William Osler Health System, Brampton, Canada
               type:PostalAddress
            type:Organization
      name:Pedro Castelo-Branco
      affiliation:
            name:University of Algarve
            address:
               name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
               type:PostalAddress
            type:Organization
            name:University of Algarve
            address:
               name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
               type:PostalAddress
            type:Organization
            name:Campus Gambelas
            address:
               name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, Lisbon, Portugal
      name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
      name:Present address: Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Vienna, Austria
      name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
      name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
      name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
      name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
      name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
      name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal
      name:William Osler Health System, Brampton, Canada
      name:Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal
      name:Centre for Biomedical Research (CBMR), University of Algarve, Faro, Portugal
      name:Algarve Biomedical Center, Campus Gambelas, Faro, Portugal

External Links {🔗}(152)

Analytics and Tracking {📊}

  • Google Tag Manager

Libraries {📚}

  • Clipboard.js
  • Prism.js

CDN Services {📦}

  • Crossref

5.12s.