We are analyzing https://link.springer.com/article/10.1186/s12870-018-1255-z.

Title:
Salt hypersensitive mutant 9, a nucleolar APUM23 protein, is essential for salt sensitivity in association with the ABA signaling pathway in Arabidopsis | BMC Plant Biology
Description:
Background Although the nucleolus involves two major functions: pre-rRNA processing and ribosome biogenesis/assembly, increasing evidence indicates that it also plays important roles in response to abiotic stress. However, the possible regulatory mechanisms underlying the nucleolar proteins responsive to abiotic stress are largely unknown. High salinity is one of the major abiotic stresses, which hinders plant growth and productivity. Here, genetic screening approach was used to identify a salt hypersensitive mutant 9 (sahy9) mutant, also known as apum23, in Arabidopsis thaliana. Functional characterization of SAHY9/APUM23 through analyses of gene/protein expression profiles and metabolites was performed to decipher the possible regulatory mechanisms of the nucleolar protein SAHY9/APUM23 in response to salt stress. Results Seedlings of the sahy9/apum23 mutant displayed postgermination developmental arrest and then became bleached after prolonged culture under various salt stresses. Transcriptomic and proteomic analyses of salt-treated sahy9/apum23 and wild-type seedlings revealed differential expression of genes/proteins that have similar functional categories of biological processes, primarily those involved in cellular and metabolic processes as well as abiotic and biotic stress responses. However, the consistency of differential gene expression at both the transcript and protein levels was low (~ 12%), which suggests the involvement of posttranscriptional processing during the salt response. Furthermore, the altered expression of genes and proteins mediated by SAHY9/APUM23 regarding salt sensitivity involves abscisic acid (ABA) biosynthesis and signaling, abiotic stress responses, and ribosome biogenesis-related genes. Importantly, NCED3, ABI2, PP2CA, and major ABA-responsive marker genes, such as RD20 and RD29B, were down-regulated at both the transcript and protein levels in conjunction with lower contents of ABA and changes in the expression of a subset of LEA proteins in sahy9/apum23 mutants under salt stress. Moreover, the salt hypersensitivity of the sahy9/apum23 mutant was largely rescued by the exogenous application of ABA during salt stress. Conclusion Our results revealed that SAHY9/APUM23 regulated the expression of ribosome biogenesis-related genes and proteins, which further affected the ribosome composition and abundance, and potential posttranscriptional regulation. The salt hypersensitivity of sahy9/apum23 is associated with the ABA-mediated signaling pathway and the downstream stress-responsive network of this pathway.
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Keywords {🔍}

salt, stress, sahyapum, expression, proteins, genes, aba, conditions, article, protein, pubmed, plant, google, scholar, ribosome, cas, mutants, mutant, seedlings, gene, arabidopsis, fig, involved, apum, differentially, expressed, plants, nacl, grown, biogenesis, type, wild, normal, table, signaling, response, medium, analysis, lea, study, growth, levels, additional, data, abiotic, file, central, pathway, days, differential,

Topics {✒️}

t-dna-inserted flanking sequences wan-hsing cheng quantitative real-time pcr aba-mediated downstream stress-responsive sahy9/apum23-mediated salt sensitivity post-germination developmental arrest pyr/pyl/rcar receptors sahy9/apum23-mediated gene expression article download pdf enzyme-linked immunosorbent assay cbl-interacting protein kinases sahy9/apum23-mediated salt response t-dna insertion sites downstream stress-responsive network rt-pcr assay revealed genome-wide insertional mutagenesis puf protein-mediated deadenylation salt-resistant phenotypes remains t-dna insertion site domain rna-binding proteins salt overly sensitive downstream stress-responsive genes late embryogenesis abundant sahy9/apum23 alters sensitivity aba-mediated gene expression pre-ribosomal rna processing stress-responsive marker genes aba-responsive marker genes nucleolar protein sahy9/apum23 sahy9/apum23 mutanta compared aba-mediated signaling pathway gene/protein expression profiles salt-treated sahy9/apum23 sahy9/apum23 mutant phenotype ribosome biogenesis-related genes small plant size sahy9/apum23 mutants compared abiotic stress-related genes cbl-cipk network pyr/pyl family sahy9/apum23 mutant seedlings cbl-cipk mediates arabidopsis thaliana reveals full access published article downstream stress-responsive 60s acidic rp privacy choices/manage cookies similar phenotypes consisting alter ribosome biogenesis/assembly

Questions {❓}

The PUF family of RNA binding proteins: does evolutionarily conserved structure equal conserved function?

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         description:Although the nucleolus involves two major functions: pre-rRNA processing and ribosome biogenesis/assembly, increasing evidence indicates that it also plays important roles in response to abiotic stress. However, the possible regulatory mechanisms underlying the nucleolar proteins responsive to abiotic stress are largely unknown. High salinity is one of the major abiotic stresses, which hinders plant growth and productivity. Here, genetic screening approach was used to identify a salt hypersensitive mutant 9 (sahy9) mutant, also known as apum23, in Arabidopsis thaliana. Functional characterization of SAHY9/APUM23 through analyses of gene/protein expression profiles and metabolites was performed to decipher the possible regulatory mechanisms of the nucleolar protein SAHY9/APUM23 in response to salt stress. Seedlings of the sahy9/apum23 mutant displayed postgermination developmental arrest and then became bleached after prolonged culture under various salt stresses. Transcriptomic and proteomic analyses of salt-treated sahy9/apum23 and wild-type seedlings revealed differential expression of genes/proteins that have similar functional categories of biological processes, primarily those involved in cellular and metabolic processes as well as abiotic and biotic stress responses. However, the consistency of differential gene expression at both the transcript and protein levels was low (~ 12%), which suggests the involvement of posttranscriptional processing during the salt response. Furthermore, the altered expression of genes and proteins mediated by SAHY9/APUM23 regarding salt sensitivity involves abscisic acid (ABA) biosynthesis and signaling, abiotic stress responses, and ribosome biogenesis-related genes. Importantly, NCED3, ABI2, PP2CA, and major ABA-responsive marker genes, such as RD20 and RD29B, were down-regulated at both the transcript and protein levels in conjunction with lower contents of ABA and changes in the expression of a subset of LEA proteins in sahy9/apum23 mutants under salt stress. Moreover, the salt hypersensitivity of the sahy9/apum23 mutant was largely rescued by the exogenous application of ABA during salt stress. Our results revealed that SAHY9/APUM23 regulated the expression of ribosome biogenesis-related genes and proteins, which further affected the ribosome composition and abundance, and potential posttranscriptional regulation. The salt hypersensitivity of sahy9/apum23 is associated with the ABA-mediated signaling pathway and the downstream stress-responsive network of this pathway.
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         Plant Sciences
         Agriculture
         Tree Biology
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      name:Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
      name:Institute of Plant Biology, National Taiwan University, Taipei, Taiwan
      name:Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
      name:Institute of Plant Biology, National Taiwan University, Taipei, Taiwan

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