We are analyzing https://link.springer.com/article/10.1186/s10020-023-00694-7.

Title:
Histone acetyltransferase P300 deficiency promotes ferroptosis of vascular smooth muscle cells by activating the HIF-1α/HMOX1 axis | Molecular Medicine
Description:
Background E1A-associated 300-kDa protein (P300), an endogenous histone acetyltransferase, contributes to modifications of the chromatin landscape of genes involved in multiple cardiovascular diseases. Ferroptosis of vascular smooth muscle cells (VSMCs) is a novel pathological mechanism of aortic dissection. However, whether P300 regulates VSMC ferroptosis remains unknown. Methods Cystine deprivation (CD) and imidazole ketone erastin (IKE) were used to induce VSMC ferroptosis. Two different knockdown plasmids targeting P300 and A-485 (a specific inhibitor of P300) were used to investigate the function of P300 in the ferroptosis of human aortic smooth muscle cells (HASMCs). Cell counting kit-8, lactate dehydrogenase and flow cytometry with propidium iodide staining were performed to assess the cell viability and death under the treatment of CD and IKE. BODIPY-C11 assay, immunofluorescence staining of 4-hydroxynonenal and malondialdehyde assay were conducted to detect the level of lipid peroxidation. Furthermore, co-immunoprecipitation was utilized to explore the interaction between P300 and HIF-1α, HIF-1α and P53. Results Compared with normal control, the protein level of P300 was significantly decreased in HASMCs treated with CD and IKE, which was largely nullified by the ferroptosis inhibitor ferrostatin-1 but not by the autophagy inhibitor or apoptosis inhibitor. Knockdown of P300 by short-hairpin RNA or inhibition of P300 activity by A-485 promoted CD- and IKE-induced HASMC ferroptosis, as evidenced by a reduction in cell viability and aggravation of lipid peroxidation of HASMCs. Furthermore, we found that hypoxia-inducible factor-1α (HIF-1α)/heme oxygenase 1 (HMOX1) pathway was responsible for the impacts of P300 on ferroptosis of HASMCs. The results of co-immunoprecipitation demonstrated that P300 and P53 competitively bound HIF-1α to regulate the expression of HMOX1. Under normal conditions, P300 interacted with HIF-1α to inhibit HMOX1 expression, while reduced expression of P300 induced by ferroptosis inducers would favor HIF-1α binding to P53 to trigger HMOX1 overexpression. Furthermore, the aggravated effects of P300 knockdown on HASMC ferroptosis were largely nullified by HIF-1α knockdown or the HIF-1α inhibitor BAY87-2243. Conclusion Thus, our results revealed that P300 deficiency or inactivation facilitated CD- and IKE-induced VSMC ferroptosis by activating the HIF-1α/HMOX1 axis, which may contribute to the development of diseases related to VSMC ferroptosis.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Health & Fitness

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,016 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't figure out the monetization strategy.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com could have a money-making trick up its sleeve, but it's undetectable for now.

Keywords {🔍}

ferroptosis, hasmcs, cell, hifα, pubmed, ike, article, cells, treatment, fig, google, scholar, protein, group, aortic, hmox, cas, assay, results, lipid, knockdown, expression, peroxidation, central, inhibitor, viability, death, treated, levels, fer, histone, mda, vascular, vsmc, staining, effects, vsmcs, dissection, kit, bodipyc, smooth, muscle, significantly, chen, min, deficiency, wei, human, level, interaction,

Topics {✒️}

jak2/pdgfr-dependent elk-1/p300 activation sk-n-mc neuroblastoma cells pencmv-p300-ha plasmid hif-1alpha/ho-1 signaling pathway hif-1α mediates ding-sheng jiang hif-1alpha/p53 direct interaction p300/hif-1α/hmox1 axis including nf-κb pathway hypoxia-induced gene activation hippo–yap signalling pathway hippo‑yap signaling pathway ming-jia ma nf-kb signaling pathways hif-1alpha/ho-1 pathway hif–1alpha inhibits ferroptosis hif-1alpha/slc7a11 pathway hypoxia-inducible factor-1α related subjects hif-1α/hmox1 axis article download pdf cold dme/f12 medium critical ferroptosis-related gene hif-1α/hmox1 pathway hif-1α forward primer ischemia/reperfusion induced cardiomyopathy targets lineage-specific tumours prechilled dme/f12 medium vitamin d3-overloaded mice favor hif-1α binding hif-1α largely reversed hif-1α reverse primer oxidized bodipy-c11 ratio regulating tnf-α expression ros/acetyl-p53 pathway iron-dependent lipid peroxidation hrp-linked secondary antibody ike-induced vsmc ferroptosis β-aminopropionitrile-induced development suppressing autophagy activation ike-induced cell viability hypoxia-responsive genes ike-induced lipid peroxidation degenerative vascular disease regulating p53/slc7a11 pathway tumor-infiltrating macrophages p300-regulated vmsc ferroptosis full access histone deacetylase inhibitors hypoxia-inducible factor

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Histone acetyltransferase P300 deficiency promotes ferroptosis of vascular smooth muscle cells by activating the HIF-1α/HMOX1 axis
         description:E1A-associated 300-kDa protein (P300), an endogenous histone acetyltransferase, contributes to modifications of the chromatin landscape of genes involved in multiple cardiovascular diseases. Ferroptosis of vascular smooth muscle cells (VSMCs) is a novel pathological mechanism of aortic dissection. However, whether P300 regulates VSMC ferroptosis remains unknown. Cystine deprivation (CD) and imidazole ketone erastin (IKE) were used to induce VSMC ferroptosis. Two different knockdown plasmids targeting P300 and A-485 (a specific inhibitor of P300) were used to investigate the function of P300 in the ferroptosis of human aortic smooth muscle cells (HASMCs). Cell counting kit-8, lactate dehydrogenase and flow cytometry with propidium iodide staining were performed to assess the cell viability and death under the treatment of CD and IKE. BODIPY-C11 assay, immunofluorescence staining of 4-hydroxynonenal and malondialdehyde assay were conducted to detect the level of lipid peroxidation. Furthermore, co-immunoprecipitation was utilized to explore the interaction between P300 and HIF-1α, HIF-1α and P53. Compared with normal control, the protein level of P300 was significantly decreased in HASMCs treated with CD and IKE, which was largely nullified by the ferroptosis inhibitor ferrostatin-1 but not by the autophagy inhibitor or apoptosis inhibitor. Knockdown of P300 by short-hairpin RNA or inhibition of P300 activity by A-485 promoted CD- and IKE-induced HASMC ferroptosis, as evidenced by a reduction in cell viability and aggravation of lipid peroxidation of HASMCs. Furthermore, we found that hypoxia-inducible factor-1α (HIF-1α)/heme oxygenase 1 (HMOX1) pathway was responsible for the impacts of P300 on ferroptosis of HASMCs. The results of co-immunoprecipitation demonstrated that P300 and P53 competitively bound HIF-1α to regulate the expression of HMOX1. Under normal conditions, P300 interacted with HIF-1α to inhibit HMOX1 expression, while reduced expression of P300 induced by ferroptosis inducers would favor HIF-1α binding to P53 to trigger HMOX1 overexpression. Furthermore, the aggravated effects of P300 knockdown on HASMC ferroptosis were largely nullified by HIF-1α knockdown or the HIF-1α inhibitor BAY87-2243. Thus, our results revealed that P300 deficiency or inactivation facilitated CD- and IKE-induced VSMC ferroptosis by activating the HIF-1α/HMOX1 axis, which may contribute to the development of diseases related to VSMC ferroptosis.
         datePublished:2023-07-06T00:00:00Z
         dateModified:2023-07-06T00:00:00Z
         pageStart:1
         pageEnd:15
         license:http://creativecommons.org/licenses/by/4.0/
         sameAs:https://doi.org/10.1186/s10020-023-00694-7
         keywords:
            Vascular smooth muscle cells
            Ferroptosis
            P300
            HIF-1α
            HMOX1
            P53
            Molecular Medicine
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig4_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig5_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig6_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig7_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig8_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig9_HTML.png
         isPartOf:
            name:Molecular Medicine
            issn:
               1528-3658
            volumeNumber:29
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Juan Shi
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Qun-Hui Wang
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xiang Wei
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
                     name: Chinese Academy of Medical Sciences
                     address:
                        name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Bo Huo
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jian-Nan Ye
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xin Yi
               affiliation:
                     name:Renmin Hospital of Wuhan University
                     address:
                        name:Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xin Feng
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ze-Min Fang
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ding-Sheng Jiang
               url:http://orcid.org/0000-0002-0393-3952
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
                     name: Chinese Academy of Medical Sciences
                     address:
                        name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Ming-Jia Ma
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Histone acetyltransferase P300 deficiency promotes ferroptosis of vascular smooth muscle cells by activating the HIF-1α/HMOX1 axis
      description:E1A-associated 300-kDa protein (P300), an endogenous histone acetyltransferase, contributes to modifications of the chromatin landscape of genes involved in multiple cardiovascular diseases. Ferroptosis of vascular smooth muscle cells (VSMCs) is a novel pathological mechanism of aortic dissection. However, whether P300 regulates VSMC ferroptosis remains unknown. Cystine deprivation (CD) and imidazole ketone erastin (IKE) were used to induce VSMC ferroptosis. Two different knockdown plasmids targeting P300 and A-485 (a specific inhibitor of P300) were used to investigate the function of P300 in the ferroptosis of human aortic smooth muscle cells (HASMCs). Cell counting kit-8, lactate dehydrogenase and flow cytometry with propidium iodide staining were performed to assess the cell viability and death under the treatment of CD and IKE. BODIPY-C11 assay, immunofluorescence staining of 4-hydroxynonenal and malondialdehyde assay were conducted to detect the level of lipid peroxidation. Furthermore, co-immunoprecipitation was utilized to explore the interaction between P300 and HIF-1α, HIF-1α and P53. Compared with normal control, the protein level of P300 was significantly decreased in HASMCs treated with CD and IKE, which was largely nullified by the ferroptosis inhibitor ferrostatin-1 but not by the autophagy inhibitor or apoptosis inhibitor. Knockdown of P300 by short-hairpin RNA or inhibition of P300 activity by A-485 promoted CD- and IKE-induced HASMC ferroptosis, as evidenced by a reduction in cell viability and aggravation of lipid peroxidation of HASMCs. Furthermore, we found that hypoxia-inducible factor-1α (HIF-1α)/heme oxygenase 1 (HMOX1) pathway was responsible for the impacts of P300 on ferroptosis of HASMCs. The results of co-immunoprecipitation demonstrated that P300 and P53 competitively bound HIF-1α to regulate the expression of HMOX1. Under normal conditions, P300 interacted with HIF-1α to inhibit HMOX1 expression, while reduced expression of P300 induced by ferroptosis inducers would favor HIF-1α binding to P53 to trigger HMOX1 overexpression. Furthermore, the aggravated effects of P300 knockdown on HASMC ferroptosis were largely nullified by HIF-1α knockdown or the HIF-1α inhibitor BAY87-2243. Thus, our results revealed that P300 deficiency or inactivation facilitated CD- and IKE-induced VSMC ferroptosis by activating the HIF-1α/HMOX1 axis, which may contribute to the development of diseases related to VSMC ferroptosis.
      datePublished:2023-07-06T00:00:00Z
      dateModified:2023-07-06T00:00:00Z
      pageStart:1
      pageEnd:15
      license:http://creativecommons.org/licenses/by/4.0/
      sameAs:https://doi.org/10.1186/s10020-023-00694-7
      keywords:
         Vascular smooth muscle cells
         Ferroptosis
         P300
         HIF-1α
         HMOX1
         P53
         Molecular Medicine
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig4_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig5_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig6_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig7_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig8_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs10020-023-00694-7/MediaObjects/10020_2023_694_Fig9_HTML.png
      isPartOf:
         name:Molecular Medicine
         issn:
            1528-3658
         volumeNumber:29
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Juan Shi
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Qun-Hui Wang
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xiang Wei
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
                  name: Chinese Academy of Medical Sciences
                  address:
                     name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Bo Huo
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jian-Nan Ye
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xin Yi
            affiliation:
                  name:Renmin Hospital of Wuhan University
                  address:
                     name:Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xin Feng
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ze-Min Fang
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ding-Sheng Jiang
            url:http://orcid.org/0000-0002-0393-3952
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
                  name: Chinese Academy of Medical Sciences
                  address:
                     name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Ming-Jia Ma
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Molecular Medicine
      issn:
         1528-3658
      volumeNumber:29
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Huazhong University of Science and Technology
      address:
         name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name: Chinese Academy of Medical Sciences
      address:
         name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name:Renmin Hospital of Wuhan University
      address:
         name:Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name: Chinese Academy of Medical Sciences
      address:
         name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Juan Shi
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Qun-Hui Wang
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Xiang Wei
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
            name: Chinese Academy of Medical Sciences
            address:
               name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Bo Huo
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Jian-Nan Ye
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Xin Yi
      affiliation:
            name:Renmin Hospital of Wuhan University
            address:
               name:Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Xin Feng
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Ze-Min Fang
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Ding-Sheng Jiang
      url:http://orcid.org/0000-0002-0393-3952
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
            name: Chinese Academy of Medical Sciences
            address:
               name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Ming-Jia Ma
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
      name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
      name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Key Laboratory of Organ Transplantation, Ministry of Education; NHC Key Laboratory of Organ Transplantation; Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China
      name:Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

External Links {🔗}(192)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

5.12s.

LINK . SPRINGER . COM {}