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Keywords {🔍}

process, sequencing, dna, number, samples, sample, automated, pubmed, additional, file, capture, catalogue, article, protocol, genome, shs, library, quality, liquid, handling, pcr, fragments, human, control, google, scholar, methods, size, selection, plate, step, targeted, method, figure, beads, pond, usa, cas, steps, data, shearing, exome, table, spri, central, manual, platform, construction, technologies, libraries,

Topics {✒️}

gov/1000genomes/ftp/pilot_data/release/2010_07/trio/snps/] dbsnp gel-free size selection agarose gel-based sizing article number r1 solution hybrid selection article download pdf full size image subsequent bead-based clean improved process control single-nucleotide polymorphism discovery sequence-ready libraries produced analysis picard pipeline hybrid selection method sequence-ready barcoded libraries filter-based hybridization capture full access bead-based cleanups increased liquid handling robot protein-coding genes implicated cost-effective targeted sequencing temperature-calibrated heating blocks solid-phase reversible immobilization bead-bait-dna complex solid-phase capture strategies support high-throughput shs cancer-related exome subset liquid handling platform adaptive focused acoustics automated high-throughput process genome-wide association studies exon kit v2 exon v2 kit liquid handler robot automated liquid handling liquid handling events agilent sureselect human liquid handling robots left-hand y-axis sequence-ready library sequence-ready libraries microtiter plate format liquid handling steps caliper gx system eluant/bead solution size quality control pe enrichment primers manufacturer-supplied dna standards robot performance issues privacy choices/manage cookies size distribution produced

Schema {🗺️}

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         headline:A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
         description:Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
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      headline:A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
      description:Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
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         Liquid Handling
         Automate Protocol
         Size Selection Step
         Liquid Handling Robot
         Microtiter Plate Format
         Animal Genetics and Genomics
         Human Genetics
         Plant Genetics and Genomics
         Microbial Genetics and Genomics
         Bioinformatics
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         name:Genetic Analysis Platform, Broad Institute of MIT and Harvard, Cambridge, USA
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      name:Broad Institute of MIT and Harvard
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         name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
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      name:Broad Institute of MIT and Harvard
      address:
         name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
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      address:
         name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
         type:PostalAddress
      name:Broad Institute of MIT and Harvard
      address:
         name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
         type:PostalAddress
      name:Broad Institute of MIT and Harvard
      address:
         name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
         type:PostalAddress
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      address:
         name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
         type:PostalAddress
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      address:
         name:Foundation Medicine, Cambridge, USA
         type:PostalAddress
      name:Broad Institute of MIT and Harvard
      address:
         name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
         type:PostalAddress
      name:Broad Institute of MIT and Harvard
      address:
         name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
         type:PostalAddress
      name:Broad Institute of MIT and Harvard
      address:
         name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
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Person:
      name:Sheila Fisher
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Andrew Barry
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Justin Abreu
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Brian Minie
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Jillian Nolan
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Toni M Delorey
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
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      name:Geneva Young
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            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
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            address:
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            address:
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            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
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            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
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      name:Brendan Blumenstiel
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
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      name:Kristian Cibulskis
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genetic Analysis Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
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      name:Dennis Friedrich
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
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      name:Ryan Johnson
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
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      name:Frank Juhn
      affiliation:
            name:Foundation Medicine
            address:
               name:Foundation Medicine, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Brian Reilly
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Ramy Shammas
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:John Stalker
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Sean M Sykes
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Jon Thompson
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:John Walsh
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Andrew Zimmer
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Zac Zwirko
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
            name:Foundation Medicine
            address:
               name:Foundation Medicine, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Stacey Gabriel
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Robert Nicol
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Chad Nusbaum
      affiliation:
            name:Broad Institute of MIT and Harvard
            address:
               name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genetic Analysis Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genetic Analysis Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Foundation Medicine, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Foundation Medicine, Cambridge, USA
      name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA

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