We are analyzing https://link.springer.com/article/10.1186/gb-2006-7-5-r40.

Title:
Large-scale and high-confidence proteomic analysis of human seminal plasma | Genome Biology
Description:
Background The development of mass spectrometric (MS) techniques now allows the investigation of very complex protein mixtures ranging from subcellular structures to tissues. Body fluids are also popular targets of proteomic analysis because of their potential for biomarker discovery. Seminal plasma has not yet received much attention from the proteomics community but its characterization could provide a future reference for virtually all studies involving human sperm. The fluid is essential for the survival of spermatozoa and their successful journey through the female reproductive tract. Results Here we report the high-confidence identification of 923 proteins in seminal fluid from a single individual. Fourier transform MS enabled parts per million mass accuracy, and two consecutive stages of MS fragmentation allowed confident identification of proteins even by single peptides. Analysis with GoMiner annotated two-thirds of the seminal fluid proteome and revealed a large number of extracellular proteins including many proteases. Other proteins originated from male accessory glands and have important roles in spermatozoan survival. Conclusion This high-confidence characterization of seminal plasma content provides an inventory of proteins with potential roles in fertilization. When combined with quantitative proteomics methodologies, it should be useful for studies of fertilization, male infertility, and prostatic and testicular cancers.
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Keywords {🔍}

proteins, seminal, protein, plasma, fluid, pubmed, article, google, scholar, data, analysis, identified, human, cas, mass, peptide, peptides, proteome, proteomics, spermatozoa, identification, number, prostate, male, additional, cancer, psa, database, function, body, present, spectra, gel, mann, studies, molecular, file, information, gominer, figure, sample, proteomic, single, fragmentation, role, high, ion, score, center, large,

Topics {✒️}

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Questions {❓}

Lwaleed BA, Greenfield R, Royle E, Birch B, Cooper AJ: Seminal factor VIII and von Willebrand factor: a possible role of the conventional clotting system in human semen?
Stewart AB, Anderson W, Delves G, Lwaleed BA, Birch B, Cooper A: Prostasomes: a role in prostatic disease?
What does this large and high-confidence set of seminal fluid proteins reveal about the function of this body fluid?
Why are a majority of proteins not annotated as secreted?

Schema {🗺️}

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         headline:Large-scale and high-confidence proteomic analysis of human seminal plasma
         description:The development of mass spectrometric (MS) techniques now allows the investigation of very complex protein mixtures ranging from subcellular structures to tissues. Body fluids are also popular targets of proteomic analysis because of their potential for biomarker discovery. Seminal plasma has not yet received much attention from the proteomics community but its characterization could provide a future reference for virtually all studies involving human sperm. The fluid is essential for the survival of spermatozoa and their successful journey through the female reproductive tract. Here we report the high-confidence identification of 923 proteins in seminal fluid from a single individual. Fourier transform MS enabled parts per million mass accuracy, and two consecutive stages of MS fragmentation allowed confident identification of proteins even by single peptides. Analysis with GoMiner annotated two-thirds of the seminal fluid proteome and revealed a large number of extracellular proteins including many proteases. Other proteins originated from male accessory glands and have important roles in spermatozoan survival. This high-confidence characterization of seminal plasma content provides an inventory of proteins with potential roles in fertilization. When combined with quantitative proteomics methodologies, it should be useful for studies of fertilization, male infertility, and prostatic and testicular cancers.
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         Evolutionary Biology
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               type:PostalAddress
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      name:Center for Experimental BioInformatics (CEBI), Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark
      name:Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany

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