We are analyzing https://link.springer.com/article/10.1186/gb-2004-5-3-r13.

Title:
Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation | Genome Biology
Description:
Background The microRNAs (miRNAs) are an extensive class of small noncoding RNAs (18 to 25 nucleotides) with probable roles in the regulation of gene expression. In Caenorhabditis elegans, lin-4 and let-7 miRNAs control the timing of fate specification of neuronal and hypodermal cells during larval development. lin-4, let-7 and other miRNA genes are conserved in mammals, and their potential functions in mammalian development are under active study. Results In order to identify mammalian miRNAs that might function in development, we characterized the expression of 119 previously reported miRNAs in adult organs from mouse and human using northern blot analysis. Of these, 30 miRNAs were specifically expressed or greatly enriched in a particular organ (brain, lung, liver or skeletal muscle). This suggests organ- or tissue-specific functions for miRNAs. To test if any of the 66 brain-expressed miRNAs were present in neurons, embryonal carcinoma cells were treated with all-trans-retinoic acid to promote neuronal differentiation. A total of 19 brain-expressed miRNAs (including lin-4 and let-7 orthologs) were coordinately upregulated in both human and mouse embryonal carcinoma cells during neuronal differentiation. The mammalian ortholog of C. elegans lin-28, which is downregulated by lin-4 in worms via 3
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Technology & Computing

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,643,078 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't figure out the monetization strategy.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com might be cashing in, but we can't detect the method they're using.

Keywords {🔍}

mirnas, lin, expression, cells, article, google, scholar, human, mouse, mirna, neuronal, organs, expressed, differentiation, cell, rna, ntd, northern, mammalian, gene, development, brain, figure, levels, biol, data, organ, detected, micrornas, conserved, predicted, utr, profiles, analysis, neurons, similar, target, cloned, brainexpressed, ambros, genes, treated, role, small, regulation, muscle, embryonal, regulatory, downregulation, sequence,

Topics {✒️}

mir-23b~mir-27b~mir-24/mir-189 exhibited [α-32p]-datp 6000 ci/mmol mir-23b~mir-27b~mir-24/mir-189 chronic lymphocytic leukemia aggressive b-cell leukemia pro-apoptotic gene hid health grant t32-ca09658 shared cis-regulatory elements mir-1d~mir-133 hairpin precursors article number r13 additional data file dartmouth-hitchcock medical center retinoic acid induces micro-rna genes mir15 size-selected rna libraries broad-complex gene activity trans-retinoic acid mir-142s/mir-142as nt2/d1 cell lines controls cell proliferation ian pitha-rowe ra-induced differentiation parallels references lagos-quintana controls temporal patterning nt2/d1 cells treated gene regulatory networks tumor suppressor gene privacy choices/manage cookies brain-enriched mirnas compared identify additional classes represent closely linked embryonal carcinoma cells trigger transcript degradation pluripotent cell state native lin-28 gene neuron-expressed mir-125b human embryonal carcinoma related subjects testing 20-nts segments biotechnology information[http dartmouth medical school skeletal muscle-enriched mirnas microrna-298-5p released supplementary regulatory role closely linked mirnas developmentally regulated microrna full size image representative ra-induced mirnas rna-rna interaction northern blot analysis

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation
         description:The microRNAs (miRNAs) are an extensive class of small noncoding RNAs (18 to 25 nucleotides) with probable roles in the regulation of gene expression. In Caenorhabditis elegans, lin-4 and let-7 miRNAs control the timing of fate specification of neuronal and hypodermal cells during larval development. lin-4, let-7 and other miRNA genes are conserved in mammals, and their potential functions in mammalian development are under active study. In order to identify mammalian miRNAs that might function in development, we characterized the expression of 119 previously reported miRNAs in adult organs from mouse and human using northern blot analysis. Of these, 30 miRNAs were specifically expressed or greatly enriched in a particular organ (brain, lung, liver or skeletal muscle). This suggests organ- or tissue-specific functions for miRNAs. To test if any of the 66 brain-expressed miRNAs were present in neurons, embryonal carcinoma cells were treated with all-trans-retinoic acid to promote neuronal differentiation. A total of 19 brain-expressed miRNAs (including lin-4 and let-7 orthologs) were coordinately upregulated in both human and mouse embryonal carcinoma cells during neuronal differentiation. The mammalian ortholog of C. elegans lin-28, which is downregulated by lin-4 in worms via 3' untranslated region binding, was also repressed during neuronal differentiation of mammalian embryonal carcinoma cells. Mammalian lin-28 messenger RNAs contain conserved predicted binding sites in their 3' untranslated regions for neuron-expressed miR-125b (a lin-4 ortholog), let-7a, and miR-218. The identification of a subset of brain-expressed miRNAs whose expression behavior is conserved in both mouse and human differentiating neurons implicates these miRNAs in mammalian neuronal development or function.
         datePublished:2004-02-16T00:00:00Z
         dateModified:2004-02-16T00:00:00Z
         pageStart:1
         pageEnd:11
         sameAs:https://doi.org/10.1186/gb-2004-5-3-r13
         keywords:
            Chronic Lymphocytic Leukemia
            Neuronal Differentiation
            Additional Data File
            miRNA Gene
            Northern Analysis
            Animal Genetics and Genomics
            Human Genetics
            Plant Genetics and Genomics
            Microbial Genetics and Genomics
            Bioinformatics
            Evolutionary Biology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig1_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig2_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig3_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig4_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig5_HTML.jpg
         isPartOf:
            name:Genome Biology
            issn:
               1474-760X
            volumeNumber:5
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Lorenzo F Sempere
               affiliation:
                     name:Dartmouth Medical School
                     address:
                        name:Department of Genetics, Dartmouth Medical School, Hanover, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Sarah Freemantle
               affiliation:
                     name:Dartmouth Medical School
                     address:
                        name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ian Pitha-Rowe
               affiliation:
                     name:Dartmouth Medical School
                     address:
                        name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Eric Moss
               affiliation:
                     name:University of Medicine and Dentistry of NJ
                     address:
                        name:Department of Molecular Biology, University of Medicine and Dentistry of NJ, Stratford, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ethan Dmitrovsky
               affiliation:
                     name:Dartmouth Medical School
                     address:
                        name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
                        type:PostalAddress
                     type:Organization
                     name:Dartmouth-Hitchcock Medical Center
                     address:
                        name:Department of Medicine and Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Victor Ambros
               affiliation:
                     name:Dartmouth Medical School
                     address:
                        name:Department of Genetics, Dartmouth Medical School, Hanover, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation
      description:The microRNAs (miRNAs) are an extensive class of small noncoding RNAs (18 to 25 nucleotides) with probable roles in the regulation of gene expression. In Caenorhabditis elegans, lin-4 and let-7 miRNAs control the timing of fate specification of neuronal and hypodermal cells during larval development. lin-4, let-7 and other miRNA genes are conserved in mammals, and their potential functions in mammalian development are under active study. In order to identify mammalian miRNAs that might function in development, we characterized the expression of 119 previously reported miRNAs in adult organs from mouse and human using northern blot analysis. Of these, 30 miRNAs were specifically expressed or greatly enriched in a particular organ (brain, lung, liver or skeletal muscle). This suggests organ- or tissue-specific functions for miRNAs. To test if any of the 66 brain-expressed miRNAs were present in neurons, embryonal carcinoma cells were treated with all-trans-retinoic acid to promote neuronal differentiation. A total of 19 brain-expressed miRNAs (including lin-4 and let-7 orthologs) were coordinately upregulated in both human and mouse embryonal carcinoma cells during neuronal differentiation. The mammalian ortholog of C. elegans lin-28, which is downregulated by lin-4 in worms via 3' untranslated region binding, was also repressed during neuronal differentiation of mammalian embryonal carcinoma cells. Mammalian lin-28 messenger RNAs contain conserved predicted binding sites in their 3' untranslated regions for neuron-expressed miR-125b (a lin-4 ortholog), let-7a, and miR-218. The identification of a subset of brain-expressed miRNAs whose expression behavior is conserved in both mouse and human differentiating neurons implicates these miRNAs in mammalian neuronal development or function.
      datePublished:2004-02-16T00:00:00Z
      dateModified:2004-02-16T00:00:00Z
      pageStart:1
      pageEnd:11
      sameAs:https://doi.org/10.1186/gb-2004-5-3-r13
      keywords:
         Chronic Lymphocytic Leukemia
         Neuronal Differentiation
         Additional Data File
         miRNA Gene
         Northern Analysis
         Animal Genetics and Genomics
         Human Genetics
         Plant Genetics and Genomics
         Microbial Genetics and Genomics
         Bioinformatics
         Evolutionary Biology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig1_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig2_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig3_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig4_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fgb-2004-5-3-r13/MediaObjects/13059_2003_Article_817_Fig5_HTML.jpg
      isPartOf:
         name:Genome Biology
         issn:
            1474-760X
         volumeNumber:5
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Lorenzo F Sempere
            affiliation:
                  name:Dartmouth Medical School
                  address:
                     name:Department of Genetics, Dartmouth Medical School, Hanover, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Sarah Freemantle
            affiliation:
                  name:Dartmouth Medical School
                  address:
                     name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ian Pitha-Rowe
            affiliation:
                  name:Dartmouth Medical School
                  address:
                     name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Eric Moss
            affiliation:
                  name:University of Medicine and Dentistry of NJ
                  address:
                     name:Department of Molecular Biology, University of Medicine and Dentistry of NJ, Stratford, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ethan Dmitrovsky
            affiliation:
                  name:Dartmouth Medical School
                  address:
                     name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
                     type:PostalAddress
                  type:Organization
                  name:Dartmouth-Hitchcock Medical Center
                  address:
                     name:Department of Medicine and Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Victor Ambros
            affiliation:
                  name:Dartmouth Medical School
                  address:
                     name:Department of Genetics, Dartmouth Medical School, Hanover, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Genome Biology
      issn:
         1474-760X
      volumeNumber:5
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Dartmouth Medical School
      address:
         name:Department of Genetics, Dartmouth Medical School, Hanover, USA
         type:PostalAddress
      name:Dartmouth Medical School
      address:
         name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
         type:PostalAddress
      name:Dartmouth Medical School
      address:
         name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
         type:PostalAddress
      name:University of Medicine and Dentistry of NJ
      address:
         name:Department of Molecular Biology, University of Medicine and Dentistry of NJ, Stratford, USA
         type:PostalAddress
      name:Dartmouth Medical School
      address:
         name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
         type:PostalAddress
      name:Dartmouth-Hitchcock Medical Center
      address:
         name:Department of Medicine and Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, USA
         type:PostalAddress
      name:Dartmouth Medical School
      address:
         name:Department of Genetics, Dartmouth Medical School, Hanover, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Lorenzo F Sempere
      affiliation:
            name:Dartmouth Medical School
            address:
               name:Department of Genetics, Dartmouth Medical School, Hanover, USA
               type:PostalAddress
            type:Organization
      name:Sarah Freemantle
      affiliation:
            name:Dartmouth Medical School
            address:
               name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
               type:PostalAddress
            type:Organization
      name:Ian Pitha-Rowe
      affiliation:
            name:Dartmouth Medical School
            address:
               name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
               type:PostalAddress
            type:Organization
      name:Eric Moss
      affiliation:
            name:University of Medicine and Dentistry of NJ
            address:
               name:Department of Molecular Biology, University of Medicine and Dentistry of NJ, Stratford, USA
               type:PostalAddress
            type:Organization
      name:Ethan Dmitrovsky
      affiliation:
            name:Dartmouth Medical School
            address:
               name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
               type:PostalAddress
            type:Organization
            name:Dartmouth-Hitchcock Medical Center
            address:
               name:Department of Medicine and Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, USA
               type:PostalAddress
            type:Organization
      name:Victor Ambros
      affiliation:
            name:Dartmouth Medical School
            address:
               name:Department of Genetics, Dartmouth Medical School, Hanover, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Genetics, Dartmouth Medical School, Hanover, USA
      name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
      name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
      name:Department of Molecular Biology, University of Medicine and Dentistry of NJ, Stratford, USA
      name:Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, USA
      name:Department of Medicine and Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, USA
      name:Department of Genetics, Dartmouth Medical School, Hanover, USA

External Links {🔗}(134)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.64s.

LINK . SPRINGER . COM {}