We are analyzing https://link.springer.com/article/10.1186/cc13970.

Title:
Receptor-interacting protein kinase 3 deficiency inhibits immune cell infiltration and attenuates organ injury in sepsis | Critical Care
Description:
Introduction Sepsis is defined as a systemic hyper-inflammatory immune response, with a subsequent immune-suppressive phase, which leads to multiple organ dysfunction and late lethality. Receptor-interacting protein kinase 3 (RIPK3)-dependent necrosis is implicated in driving tumor necrosis factor alpha (TNF-α)- and sepsis-induced mortality in mice. However, it is unknown if RIPK3 deficiency has any impact on immune cell trafficking, which contributes to organ damage in sepsis. Methods To study this, male wild-type (WT) and RIPK3-deficient (Ripk3 -/-) mice on C57BL/6 background were subjected to sham operation or cecal ligation and puncture (CLP)-induced sepsis. Blood and tissue samples were collected 20 hours post-CLP for various measurements. Results In our severe sepsis model, the mean survival time of Ripk3 -/- mice was significantly extended to 68 hours compared to 41 hours for WT mice. Ripk3 -/- mice had significantly decreased plasma levels of TNF-α and IL-6 and organ injury markers compared to WT mice post-CLP. In the lungs, Ripk3 -/- mice preserved better integrity of microscopic structure with reduced apoptosis, and decreased levels of IL-6, macrophage inflammatory protein (MIP)-2 and keratinocyte-derived chemokine (KC), compared to WT. In the liver, the levels of MIP-1, MIP-2 and KC were also decreased in septic Ripk3 -/- mice. Particularly, the total number of neutrophils in the lungs and liver of Ripk3 -/- mice decreased by 59.9% and 66.7%, respectively, compared to WT mice post-CLP. In addition, the number of natural killer (NK) and CD8T cells in the liver decreased by 64.8% and 53.4%, respectively, in Ripk3 -/- mice compared to WT mice post-sepsis. Conclusions Our data suggest that RIPK3 deficiency modestly protected from CLP-induced severe sepsis and altered the immune cell trafficking in an organ-specific manner attenuating organ injury. Thus, RIPK3 acts as a detrimental factor in contributing to the organ deterioration in sepsis.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Health & Fitness
Pets

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,626,932 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure how the site profits.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com might be making money, but it's not detectable how they're doing it.

Keywords {🔍}

mice, ripk, sepsis, cells, clp, pubmed, article, cell, liver, septic, lung, google, scholar, injury, figure, deficiency, sham, cas, organ, infiltration, lungs, data, necrosis, compared, levels, clpinduced, apoptosis, death, immune, survival, cdt, usa, show, hours, tissues, protein, study, severe, reduced, mip, analysis, hepatic, increased, neutrophil, wang, decreased, central, number, significantly, care,

Topics {✒️}

cold-inducible rna-binding protein 5% hexa-decyl-trimethyl-ammonium bromide real-time rt-pcr analysis normal nf-kappa bs activates nf-kappab n-terminal kinase domain serine/threonine protein kinases article download pdf anti-mouse abs conjugated enzyme-linked immunosorbent assay zhimin wang & ping wang mouse β-actin mrna clp-induced acute sepsis subsequent immune-suppressive phase polymicrobial intra-abdominal sepsis chemokine-mediated neutrophil infiltration neutrophil-attracting chemokine expression block fcγrii/iii receptors clp-induced polymicrobial sepsis 12 hour light/dark cycle sepsis-induced cell death intra-alveolar leukocyte infiltration unique c-terminal domain full size image migrating natural killer clp-induced sepsis specifically acute lung injury reduces systemic injury systemic inflammatory response related subjects tnf-α-induced apoptosis attenuates organ injury surface gr-1/cd11b expression clp-induced severe sepsis wt mice post-sepsis induces fasl-mediated inflammation lethal intraabdominal sepsis acute multiorgan failure macrophage inflammatory protein wt mice post-clp rt-pcr increased immune cell trafficking de almagro mc sepsis-induced liver damage organ injury markers ordered cellular explosion local proinflammatory cytokine ripk3 deficiency resulted reduces systemic levels attenuated acute lung

Questions {❓}

Ayala A, Evans TA, Chaudry IH: Does hepatocellular injury in sepsis involve apoptosis?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Receptor-interacting protein kinase 3 deficiency inhibits immune cell infiltration and attenuates organ injury in sepsis
         description:Sepsis is defined as a systemic hyper-inflammatory immune response, with a subsequent immune-suppressive phase, which leads to multiple organ dysfunction and late lethality. Receptor-interacting protein kinase 3 (RIPK3)-dependent necrosis is implicated in driving tumor necrosis factor alpha (TNF-α)- and sepsis-induced mortality in mice. However, it is unknown if RIPK3 deficiency has any impact on immune cell trafficking, which contributes to organ damage in sepsis. To study this, male wild-type (WT) and RIPK3-deficient (Ripk3 -/-) mice on C57BL/6 background were subjected to sham operation or cecal ligation and puncture (CLP)-induced sepsis. Blood and tissue samples were collected 20 hours post-CLP for various measurements. In our severe sepsis model, the mean survival time of Ripk3 -/- mice was significantly extended to 68 hours compared to 41 hours for WT mice. Ripk3 -/- mice had significantly decreased plasma levels of TNF-α and IL-6 and organ injury markers compared to WT mice post-CLP. In the lungs, Ripk3 -/- mice preserved better integrity of microscopic structure with reduced apoptosis, and decreased levels of IL-6, macrophage inflammatory protein (MIP)-2 and keratinocyte-derived chemokine (KC), compared to WT. In the liver, the levels of MIP-1, MIP-2 and KC were also decreased in septic Ripk3 -/- mice. Particularly, the total number of neutrophils in the lungs and liver of Ripk3 -/- mice decreased by 59.9% and 66.7%, respectively, compared to WT mice post-CLP. In addition, the number of natural killer (NK) and CD8T cells in the liver decreased by 64.8% and 53.4%, respectively, in Ripk3 -/- mice compared to WT mice post-sepsis. Our data suggest that RIPK3 deficiency modestly protected from CLP-induced severe sepsis and altered the immune cell trafficking in an organ-specific manner attenuating organ injury. Thus, RIPK3 acts as a detrimental factor in contributing to the organ deterioration in sepsis.
         datePublished:2014-07-04T00:00:00Z
         dateModified:2014-07-04T00:00:00Z
         pageStart:1
         pageEnd:13
         license:http://creativecommons.org/licenses/by/4.0/
         sameAs:https://doi.org/10.1186/cc13970
         keywords:
            Natural Killer Cell
            Wild Type Mouse
            Acute Lung Injury
            Septic Mouse
            Polymicrobial Sepsis
            Intensive / Critical Care Medicine
            Emergency Medicine
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig1_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig2_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig3_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig4_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig5_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig6_HTML.jpg
         isPartOf:
            name:Critical Care
            issn:
               1364-8535
            volumeNumber:18
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Archna Sharma
               affiliation:
                     name:The Feinstein Institute for Medical Research
                     address:
                        name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                        type:PostalAddress
                     type:Organization
                     name:Hofstra North Shore-LIJ School of Medicine
                     address:
                        name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Shingo Matsuo
               affiliation:
                     name:The Feinstein Institute for Medical Research
                     address:
                        name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                        type:PostalAddress
                     type:Organization
                     name:Hofstra North Shore-LIJ School of Medicine
                     address:
                        name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                        type:PostalAddress
                     type:Organization
                     name:Tokyo Women’s Medical University
                     address:
                        name:Department of Surgery II, Tokyo Women’s Medical University, Tokyo, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Weng-Lang Yang
               affiliation:
                     name:The Feinstein Institute for Medical Research
                     address:
                        name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                        type:PostalAddress
                     type:Organization
                     name:Hofstra North Shore-LIJ School of Medicine
                     address:
                        name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Zhimin Wang
               affiliation:
                     name:The Feinstein Institute for Medical Research
                     address:
                        name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                        type:PostalAddress
                     type:Organization
                     name:Hofstra North Shore-LIJ School of Medicine
                     address:
                        name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ping Wang
               affiliation:
                     name:The Feinstein Institute for Medical Research
                     address:
                        name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                        type:PostalAddress
                     type:Organization
                     name:Hofstra North Shore-LIJ School of Medicine
                     address:
                        name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Receptor-interacting protein kinase 3 deficiency inhibits immune cell infiltration and attenuates organ injury in sepsis
      description:Sepsis is defined as a systemic hyper-inflammatory immune response, with a subsequent immune-suppressive phase, which leads to multiple organ dysfunction and late lethality. Receptor-interacting protein kinase 3 (RIPK3)-dependent necrosis is implicated in driving tumor necrosis factor alpha (TNF-α)- and sepsis-induced mortality in mice. However, it is unknown if RIPK3 deficiency has any impact on immune cell trafficking, which contributes to organ damage in sepsis. To study this, male wild-type (WT) and RIPK3-deficient (Ripk3 -/-) mice on C57BL/6 background were subjected to sham operation or cecal ligation and puncture (CLP)-induced sepsis. Blood and tissue samples were collected 20 hours post-CLP for various measurements. In our severe sepsis model, the mean survival time of Ripk3 -/- mice was significantly extended to 68 hours compared to 41 hours for WT mice. Ripk3 -/- mice had significantly decreased plasma levels of TNF-α and IL-6 and organ injury markers compared to WT mice post-CLP. In the lungs, Ripk3 -/- mice preserved better integrity of microscopic structure with reduced apoptosis, and decreased levels of IL-6, macrophage inflammatory protein (MIP)-2 and keratinocyte-derived chemokine (KC), compared to WT. In the liver, the levels of MIP-1, MIP-2 and KC were also decreased in septic Ripk3 -/- mice. Particularly, the total number of neutrophils in the lungs and liver of Ripk3 -/- mice decreased by 59.9% and 66.7%, respectively, compared to WT mice post-CLP. In addition, the number of natural killer (NK) and CD8T cells in the liver decreased by 64.8% and 53.4%, respectively, in Ripk3 -/- mice compared to WT mice post-sepsis. Our data suggest that RIPK3 deficiency modestly protected from CLP-induced severe sepsis and altered the immune cell trafficking in an organ-specific manner attenuating organ injury. Thus, RIPK3 acts as a detrimental factor in contributing to the organ deterioration in sepsis.
      datePublished:2014-07-04T00:00:00Z
      dateModified:2014-07-04T00:00:00Z
      pageStart:1
      pageEnd:13
      license:http://creativecommons.org/licenses/by/4.0/
      sameAs:https://doi.org/10.1186/cc13970
      keywords:
         Natural Killer Cell
         Wild Type Mouse
         Acute Lung Injury
         Septic Mouse
         Polymicrobial Sepsis
         Intensive / Critical Care Medicine
         Emergency Medicine
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig1_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig2_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig3_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig4_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig5_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fcc13970/MediaObjects/13054_2014_Article_2588_Fig6_HTML.jpg
      isPartOf:
         name:Critical Care
         issn:
            1364-8535
         volumeNumber:18
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Archna Sharma
            affiliation:
                  name:The Feinstein Institute for Medical Research
                  address:
                     name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                     type:PostalAddress
                  type:Organization
                  name:Hofstra North Shore-LIJ School of Medicine
                  address:
                     name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Shingo Matsuo
            affiliation:
                  name:The Feinstein Institute for Medical Research
                  address:
                     name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                     type:PostalAddress
                  type:Organization
                  name:Hofstra North Shore-LIJ School of Medicine
                  address:
                     name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                     type:PostalAddress
                  type:Organization
                  name:Tokyo Women’s Medical University
                  address:
                     name:Department of Surgery II, Tokyo Women’s Medical University, Tokyo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Weng-Lang Yang
            affiliation:
                  name:The Feinstein Institute for Medical Research
                  address:
                     name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                     type:PostalAddress
                  type:Organization
                  name:Hofstra North Shore-LIJ School of Medicine
                  address:
                     name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Zhimin Wang
            affiliation:
                  name:The Feinstein Institute for Medical Research
                  address:
                     name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                     type:PostalAddress
                  type:Organization
                  name:Hofstra North Shore-LIJ School of Medicine
                  address:
                     name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ping Wang
            affiliation:
                  name:The Feinstein Institute for Medical Research
                  address:
                     name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
                     type:PostalAddress
                  type:Organization
                  name:Hofstra North Shore-LIJ School of Medicine
                  address:
                     name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Critical Care
      issn:
         1364-8535
      volumeNumber:18
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:The Feinstein Institute for Medical Research
      address:
         name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
         type:PostalAddress
      name:Hofstra North Shore-LIJ School of Medicine
      address:
         name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
         type:PostalAddress
      name:The Feinstein Institute for Medical Research
      address:
         name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
         type:PostalAddress
      name:Hofstra North Shore-LIJ School of Medicine
      address:
         name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
         type:PostalAddress
      name:Tokyo Women’s Medical University
      address:
         name:Department of Surgery II, Tokyo Women’s Medical University, Tokyo, Japan
         type:PostalAddress
      name:The Feinstein Institute for Medical Research
      address:
         name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
         type:PostalAddress
      name:Hofstra North Shore-LIJ School of Medicine
      address:
         name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
         type:PostalAddress
      name:The Feinstein Institute for Medical Research
      address:
         name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
         type:PostalAddress
      name:Hofstra North Shore-LIJ School of Medicine
      address:
         name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
         type:PostalAddress
      name:The Feinstein Institute for Medical Research
      address:
         name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
         type:PostalAddress
      name:Hofstra North Shore-LIJ School of Medicine
      address:
         name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Archna Sharma
      affiliation:
            name:The Feinstein Institute for Medical Research
            address:
               name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
               type:PostalAddress
            type:Organization
            name:Hofstra North Shore-LIJ School of Medicine
            address:
               name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
               type:PostalAddress
            type:Organization
      name:Shingo Matsuo
      affiliation:
            name:The Feinstein Institute for Medical Research
            address:
               name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
               type:PostalAddress
            type:Organization
            name:Hofstra North Shore-LIJ School of Medicine
            address:
               name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
               type:PostalAddress
            type:Organization
            name:Tokyo Women’s Medical University
            address:
               name:Department of Surgery II, Tokyo Women’s Medical University, Tokyo, Japan
               type:PostalAddress
            type:Organization
      name:Weng-Lang Yang
      affiliation:
            name:The Feinstein Institute for Medical Research
            address:
               name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
               type:PostalAddress
            type:Organization
            name:Hofstra North Shore-LIJ School of Medicine
            address:
               name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
               type:PostalAddress
            type:Organization
      name:Zhimin Wang
      affiliation:
            name:The Feinstein Institute for Medical Research
            address:
               name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
               type:PostalAddress
            type:Organization
            name:Hofstra North Shore-LIJ School of Medicine
            address:
               name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
               type:PostalAddress
            type:Organization
      name:Ping Wang
      affiliation:
            name:The Feinstein Institute for Medical Research
            address:
               name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
               type:PostalAddress
            type:Organization
            name:Hofstra North Shore-LIJ School of Medicine
            address:
               name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
      name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
      name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
      name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
      name:Department of Surgery II, Tokyo Women’s Medical University, Tokyo, Japan
      name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
      name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
      name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
      name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA
      name:Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, USA
      name:Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, USA

External Links {🔗}(174)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

5.34s.

LINK . SPRINGER . COM {}