We are analyzing https://link.springer.com/article/10.1186/bcr3574.

Title:
Cytochrome P450 2E1 (CYP2E1) regulates the response to oxidative stress and migration of breast cancer cells | Breast Cancer Research
Description:
Introduction The cytochrome P450 (CYP) enzymes are a class of heme-containing enzymes involved in phase I metabolism of a large number of xenobiotics. The CYP family member CYP2E1 metabolises many xenobiotics and pro-carcinogens, it is not just expressed in the liver but also in many other tissues such as the kidney, the lung, the brain, the gastrointestinal tract and the breast tissue. It is induced in several pathological conditions including cancer, obesity, and type II diabetes implying that this enzyme is implicated in other biological processes beyond its role in phase I metabolism. Despite the detailed description of the role of CYP2E1 in the liver, its functions in other tissues have not been extensively studied. In this study, we investigated the functional significance of CYP2E1 in breast carcinogenesis. Methods Cellular levels of reactive oxygen species (ROS) were measured by H2DCFDA (2 2.9.2 2′,7′-dichlorodihydrofluorescein diacetate) staining and autophagy was assessed by tracing the cellular levels of autophagy markers using western blot assays. The endoplasmic reticulum stress and the unfolded protein response (UPR) were detected by luciferase assays reflecting the splicing of mRNA encoding the X-box binding protein 1 (XBP1) transcription factor and cell migration was evaluated using the scratch wound assay. Gene expression was recorded with standard transcription assays including luciferase reporter and chromatin immunoprecipitation. Results Ectopic expression of CYP2E1 induced ROS generation, affected autophagy, stimulated endoplasmic reticulum stress and inhibited migration in breast cancer cells with different metastatic potential and p53 status. Furthermore, evidence is presented indicating that CYP2E1 gene expression is under the transcriptional control of the p53 tumor suppressor. Conclusions These results support the notion that CYP2E1 exerts an important role in mammary carcinogenesis, provide a potential link between ethanol metabolism and breast cancer and suggest that progression, and metastasis, of advanced stages of breast cancer can be modulated by induction of CYP2E1 activity.
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Keywords {🔍}

cype, cells, cancer, pubmed, breast, article, google, scholar, cas, mdamb, cell, expression, mcf, stress, levels, figure, protein, ros, migration, autophagy, bar, compare, gene, cytochrome, role, oxidative, endoplasmic, reticulum, regulation, activity, central, cellular, response, transfected, transcriptional, analysis, ethanol, liver, treated, luciferase, generation, increased, lanes, metabolism, upr, bars, authors, enzyme, unfolded, binding,

Topics {✒️}

apc-h7-conjugated cd20 antibody apc-conjugated anti-cd20 antibody anti-ccaat/enhancer-binding protein p53-cyp2e1-ros generation axis mda-mb-231and mda-mb-468 article download pdf fluorescence profile reactive oxygen species fluorescence-activated cell sorting endoplasmic reticulum stress-indicators ccaat/enhancer-binding protein constantinos demonacos cyan-adp flow cytometer cyp2e1-mediated ros generation jnk mitogen-activated kinases highly invasive mda-mb-231 proteasome-mediated stathmin degradation full size image cell-type-dependent manner anti-β-actin antibody mda-mb-231 cell migration invasive mda-mb-231 cells author information authors pro-oxidant p53 outcomes 20 mm β-glycerol phosphate mda-mb-231 cells migrating 50 mm tris-hcl ph 7 protein-folding process resulting stress-responsive transcription factors cyp2e1-mediated er stress endoplasmic reticulum kinase breast tumors compared view cmv-cyp2e1 expression plasmid tnf alpha-induced toxicity anti-β-actin antibodies highly reactive properties antioxid redox signal st clair dk endoplasmic reticulum stress mda-mb-157 cells treated mda-mb-231 cells treated prevents p53-dependent apoptosis mda-mb-231 cells transfected related subjects estrogen receptor [74 ectopically expressing cyp2e1 including breast cancer proline-rich domain endoplasmic reticulum ubiquitination

Questions {❓}

Malhotra JD, Kaufman RJ: Endoplasmic reticulum stress and oxidative stress: a vicious cycle or a double-edged sword?
Morgan ET: Regulation of cytochrome p450 by inflammatory mediators: why and how?

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