We are analyzing https://link.springer.com/article/10.1186/bcr306.

Title:
In vivo cell kinetics in breast carcinogenesis | Breast Cancer Research
Description:
Background Disruption of the balance between apoptosis and proliferation is considered to be an important factor in the development and progression of tumours. In the present study we determined the in vivo cell kinetics along the spectrum of apparently normal epithelium, hyperplasia, preinvasive lesions and invasive carcinoma, in breast tissues affected by fibrocystic changes in which preinvasive and/or invasive lesions developed, as a model of breast carcinogenesis. Materials and methods A total of 32 areas of apparently normal epithelium and 135 ductal proliferative and neoplastic lesions were studied. More than one epithelial lesion per case were analyzed. The apoptotic index (AI) and the proliferative index (PI) were expressed as the percentage of TdT-mediated dUTP-nick end-labelling (TUNEL) and Ki-67-positive cells, respectively. The PI/AI (P/A index) was calculated for each case. Results The AIs and PIs were significantly higher in hyperplasia than in apparently normal epithelium (P = 0.04 and P = 0.0005, respectively), in atypical hyperplasia than in hyperplasia (P = 0.01 and P = 0.04, respectively) and in invasive carcinoma than in in situ carcinoma (P < 0.001 and P < 0.001, respectively). The two indices were similar in atypical hyperplasia and in in situ carcinoma. The P/A index increased significantly from normal epithelium to hyperplasia (P = 0.01) and from preinvasive lesions to invasive carcinoma (P = 0.04) whereas it was decreased (non-significantly) from hyperplasia to preinvasive lesions. A strong positive correlation between the AIs and the PIs was found (r = 0.83, P < 0.001). Conclusion These findings suggest accelerating cell turnover along the continuum of breast carcinogenesis. Atypical hyperplasias and in situ carcinomas might be kinetically similar lesions. In the transition from normal epithelium to hyperplasia and from preinvasive lesions to invasive carcinoma the net growth of epithelial cells results from a growth imbalance in favour of proliferation. In the transition from hyperplasia to preinvasive lesions there is an imbalance in favour of apoptosis.
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Keywords {🔍}

breast, apoptosis, carcinoma, hyperplasia, cell, google, scholar, situ, invasive, article, pubmed, lesions, cells, cas, cancer, carcinomas, apoptotic, ductal, proliferation, epithelium, normal, preinvasive, proliferative, index, study, atypical, tissue, positive, slides, tunel, activity, progression, antibody, sections, epithelial, growth, pathol, carcinogenesis, ais, pis, present, higher, apparently, lesion, found, criteria, negative, results, indices, correlation,

Topics {✒️}

terminal-deoxynucleotidyl-transferase-mediated dutp-fitc nick tdt-mediated dutp-nick end-labelling poly-l-lysine-coated glass slides apoptosis avidin-biotin-peroxidase complex avidin-biotin-peroxidase complex ibm-compatible personal computer contained dutp-digoxigenin pre-menopausal human breast cyclin b1/cdc2 kinase mann-whitney tests demonstrated mann-whitney tests showed situ end-labelling proliferating-cell nuclear antigen full size image maria bai md metaplasia-dysplasia-carcinoma sequence fitc-conjugated antidigoxigenin antibody breast cancers determined breast cancer research low-grade intraepithelial neoplasia paraffin-embedded tissue samples avoid false-positive results privacy choices/manage cookies polyclonal rabbit antibody human breast cancer poorly differentiated models accelerating cell turnover steady-state condition tdt reaction step normal rabbit serum tunel-positive apoptotic cells tumor suppressor genes human ki-67 protein article bai lelle rj laryngeal carcinomas showed human hepatocellular carcinomas tunel assay showed reactive lymph nodes van diest ps adding peroxidase-conjugated cell proliferation suggest mann-whitney test static-cell population increasing cell turnover multicolor immunofluorescence reveals potent mechanisms exist eosin-stained slides phosphate-buffered saline conjugated antidigoxigenin antibody

Schema {🗺️}

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         headline:In vivo cell kinetics in breast carcinogenesis
         description:Disruption of the balance between apoptosis and proliferation is considered to be an important factor in the development and progression of tumours. In the present study we determined the in vivo cell kinetics along the spectrum of apparently normal epithelium, hyperplasia, preinvasive lesions and invasive carcinoma, in breast tissues affected by fibrocystic changes in which preinvasive and/or invasive lesions developed, as a model of breast carcinogenesis. A total of 32 areas of apparently normal epithelium and 135 ductal proliferative and neoplastic lesions were studied. More than one epithelial lesion per case were analyzed. The apoptotic index (AI) and the proliferative index (PI) were expressed as the percentage of TdT-mediated dUTP-nick end-labelling (TUNEL) and Ki-67-positive cells, respectively. The PI/AI (P/A index) was calculated for each case. The AIs and PIs were significantly higher in hyperplasia than in apparently normal epithelium (P = 0.04 and P = 0.0005, respectively), in atypical hyperplasia than in hyperplasia (P = 0.01 and P = 0.04, respectively) and in invasive carcinoma than in in situ carcinoma (P < 0.001 and P < 0.001, respectively). The two indices were similar in atypical hyperplasia and in in situ carcinoma. The P/A index increased significantly from normal epithelium to hyperplasia (P = 0.01) and from preinvasive lesions to invasive carcinoma (P = 0.04) whereas it was decreased (non-significantly) from hyperplasia to preinvasive lesions. A strong positive correlation between the AIs and the PIs was found (r = 0.83, P < 0.001). These findings suggest accelerating cell turnover along the continuum of breast carcinogenesis. Atypical hyperplasias and in situ carcinomas might be kinetically similar lesions. In the transition from normal epithelium to hyperplasia and from preinvasive lesions to invasive carcinoma the net growth of epithelial cells results from a growth imbalance in favour of proliferation. In the transition from hyperplasia to preinvasive lesions there is an imbalance in favour of apoptosis.
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      headline:In vivo cell kinetics in breast carcinogenesis
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