We are analyzing https://link.springer.com/article/10.1186/bcr2143.

Title:
ERalpha-status of disseminated tumour cells in bone marrow of primary breast cancer patients | Breast Cancer Research
Description:
Introduction Isolated disseminated tumour cells (DTC) are regarded as surrogate markers for minimal residual disease in breast cancer. Characterisation of these cells could help understand the known limitations of adjuvant therapy. Of particular interest is their oestrogen-receptor (ER) status because endocrine adjuvant therapy remains a cornerstone of breast cancer treatment. Methods Bone marrow (BM) aspirates from 254 patients with primary breast cancer were included in this study. A double immunofluorescence staining procedure was established for the identification of cytokeratin (CK) positive/Erα-positive cells. ERα status of the primary tumour was assessed immunohistochemically using the same antibody against ERα. Results In 107 of 254 (42%) breast cancer patients, CK-positive cells could be detected in the BM. More than one DTC in the BM was observed in 38 of the 107 patients. The number of detected cells ranged between 1 and 55 cells per 2 × 106 mononuclear cells. DTCs demonstrated ERα positivity in 12% of the patients. The ERα expression was heterogeneous in 10 of the 38 (26%) patients with more than one DTC. The concordance rate of ERα status between primary tumour and DTC was 28%. Only 12 of 88 patients with ERα-positive tumours also had ERα-positive DTCs. Conclusions Primary tumours and DTCs displayed a concordant ERα status in only 28% of cases. Most of the DTCs were ERα negative despite the presence of an ERα-positive primary tumour. These findings further underline the distinct nature of DTCs and may explain the failure rates seen in conventional endocrine adjuvant therapy.
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cancer, cells, breast, tumour, primary, patients, erα, dtcs, article, google, scholar, status, pubmed, cas, disseminated, erαpositive, tumours, bone, marrow, therapy, cell, staining, receptor, table, expression, solomayer, adjuvant, tumor, erαnegative, res, dtc, negative, detection, stem, endocrine, figure, metastatic, clin, analysis, fehm, antibody, germany, minutes, usa, estrogen, authors, data, full, detected, control,

Topics {✒️}

goat anti-rabbit antibody article download pdf anti-cytokeratin-fluorescein isothiocyanate ncl-l-er-6f11 cd44+/cd24- phenotypes correlated cd44+/cd24-/low cells positive/erα-positive cells erα-positive tumour cells full size image erα-positive primary tumour erα-negative tumour cells erα-positive mcf-7 cells hormone receptor-positive patients erα-positive primary tumours erα-negative primary tumour cancer stem cells erα-negative primary tumours disseminated tumour cell early breast cancer metastatic breast cancer tumour stem cells normal goat serum anti-oestrogen receptor her2-positive dtcs reflects related subjects monoclonal rabbit antibodies breast cancer treatment negative control erα-staining primary breast cancer stem/progenitor cells tumour cell isolation recurrent breast cancer advanced breast cancer monoclonal mouse antibodies alexa fluor 594 circulating tumour cells privacy choices/manage cookies natl cancer inst systemic metastatic disease full access tumour cell detection isolated disseminated cells polyclonal rabbit antibodies disseminated tumour cells breast cancer patients circulating tumor cells mcf-7 cancer cells iii breast cancer tumor cell detection breast cancer patient

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         headline:ERalpha-status of disseminated tumour cells in bone marrow of primary breast cancer patients
         description:Isolated disseminated tumour cells (DTC) are regarded as surrogate markers for minimal residual disease in breast cancer. Characterisation of these cells could help understand the known limitations of adjuvant therapy. Of particular interest is their oestrogen-receptor (ER) status because endocrine adjuvant therapy remains a cornerstone of breast cancer treatment. Bone marrow (BM) aspirates from 254 patients with primary breast cancer were included in this study. A double immunofluorescence staining procedure was established for the identification of cytokeratin (CK) positive/Erα-positive cells. ERα status of the primary tumour was assessed immunohistochemically using the same antibody against ERα. In 107 of 254 (42%) breast cancer patients, CK-positive cells could be detected in the BM. More than one DTC in the BM was observed in 38 of the 107 patients. The number of detected cells ranged between 1 and 55 cells per 2 × 106 mononuclear cells. DTCs demonstrated ERα positivity in 12% of the patients. The ERα expression was heterogeneous in 10 of the 38 (26%) patients with more than one DTC. The concordance rate of ERα status between primary tumour and DTC was 28%. Only 12 of 88 patients with ERα-positive tumours also had ERα-positive DTCs. Primary tumours and DTCs displayed a concordant ERα status in only 28% of cases. Most of the DTCs were ERα negative despite the presence of an ERα-positive primary tumour. These findings further underline the distinct nature of DTCs and may explain the failure rates seen in conventional endocrine adjuvant therapy.
         datePublished:2008-09-15T00:00:00Z
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            Primary Tumour
            Cancer Stem Cell
            Lapatinib
            Circulate Tumour Cell
            Cancer Research
            Oncology
            Surgical Oncology
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      headline:ERalpha-status of disseminated tumour cells in bone marrow of primary breast cancer patients
      description:Isolated disseminated tumour cells (DTC) are regarded as surrogate markers for minimal residual disease in breast cancer. Characterisation of these cells could help understand the known limitations of adjuvant therapy. Of particular interest is their oestrogen-receptor (ER) status because endocrine adjuvant therapy remains a cornerstone of breast cancer treatment. Bone marrow (BM) aspirates from 254 patients with primary breast cancer were included in this study. A double immunofluorescence staining procedure was established for the identification of cytokeratin (CK) positive/Erα-positive cells. ERα status of the primary tumour was assessed immunohistochemically using the same antibody against ERα. In 107 of 254 (42%) breast cancer patients, CK-positive cells could be detected in the BM. More than one DTC in the BM was observed in 38 of the 107 patients. The number of detected cells ranged between 1 and 55 cells per 2 × 106 mononuclear cells. DTCs demonstrated ERα positivity in 12% of the patients. The ERα expression was heterogeneous in 10 of the 38 (26%) patients with more than one DTC. The concordance rate of ERα status between primary tumour and DTC was 28%. Only 12 of 88 patients with ERα-positive tumours also had ERα-positive DTCs. Primary tumours and DTCs displayed a concordant ERα status in only 28% of cases. Most of the DTCs were ERα negative despite the presence of an ERα-positive primary tumour. These findings further underline the distinct nature of DTCs and may explain the failure rates seen in conventional endocrine adjuvant therapy.
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         Breast Cancer
         Primary Tumour
         Cancer Stem Cell
         Lapatinib
         Circulate Tumour Cell
         Cancer Research
         Oncology
         Surgical Oncology
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      name:Department of Obstetrics and Gynecology, University of Tuebingen, Tuebingen, Germany
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      name:Department of Obstetrics and Gynecology, University of Tuebingen, Tuebingen, Germany

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