We are analyzing https://link.springer.com/article/10.1186/bcr1656.

Title:
Induction of epithelial to mesenchymal transition in PMC42-LA human breast carcinoma cells by carcinoma-associated fibroblast secreted factors | Breast Cancer Research
Description:
Background Breast carcinoma is accompanied by changes in the acellular and cellular components of the microenvironment, the latter typified by a switch from fibroblasts to myofibroblasts. Methods We utilised conditioned media cultures, Western blot analysis and immunocytochemistry to investigate the differential effects of normal mammary fibroblasts (NMFs) and mammary cancer-associated fibroblasts (CAFs) on the phenotype and behaviour of PMC42-LA breast cancer cells. NMFs were obtained from a mammary gland at reduction mammoplasty, and CAFs from a mammary carcinoma after resection. Results We found greater expression of myofibroblastic markers in CAFs than in NMFs. Medium from both CAFs and NMFs induced novel expression of α-smooth muscle actin and cytokeratin-14 in PMC42-LA organoids. However, although conditioned media from NMFs resulted in distribution of vimentin-positive cells to the periphery of PMC42-LA organoids, this was not seen with CAF-conditioned medium. Upregulation of vimentin was accompanied by a mis-localization of E-cadherin, suggesting a loss of adhesive function. This was confirmed by visualizing the change in active β-catenin, localized to the cell junctions in control cells/cells in NMF-conditioned medium, to inactive β-catenin, localized to nuclei and cytoplasm in cells in CAF-conditioned medium. Conclusion We found no significant difference between the influences of NMFs and CAFs on PMC42-LA cell proliferation, viability, or apoptosis; significantly, we demonstrated a role for CAFs, but not for NMFs, in increasing the migratory ability of PMC42-LA cells. By concentrating NMF-conditioned media, we demonstrated the presence of factor(s) that induce epithelial-mesenchymal transition in NMF-conditioned media that are present at higher levels in CAF-conditioned media. Our in vitro results are consistent with observations in vivo showing that alterations in stroma influence the phenotype and behaviour of surrounding cells and provide evidence for a role for CAFs in stimulating cancer progression via an epithelial-mesenchymal transition. These findings have implications for our understanding of the roles of signalling between epithelial and stromal cells in the development and progression of mammary carcinoma.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Photography

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,642,828 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We find it hard to spot revenue streams.

Some websites aren't about earning revenue; they're built to connect communities or raise awareness. There are numerous motivations behind creating websites. This might be one of them. Link.springer.com has a secret sauce for making money, but we can't detect it yet.

Keywords {🔍}

cells, medium, pmcla, cell, pubmed, fibroblasts, breast, cancer, cafs, google, scholar, cas, nmfs, figure, article, expression, mammary, cultured, cafconditioned, hours, organoids, nmfconditioned, fibroblast, vimentin, human, ecadherin, control, growth, media, wound, epithelial, normal, filter, carcinoma, factors, beneath, cultures, culture, emt, conditioned, compared, increase, res, tissue, protein, part, presence, fold, factor, usa,

Topics {✒️}

beta-catenin/lef-1-mediated transactivation goat anti-human e-cadherin chemically-induced colo-rectal carcinogenesis enzyme-linked immunosorbent assay mouse anti-human vimentin article download pdf α-sma-positive stromal cells caf-conditioned media e-cadherin anti-human fap antibodies breast carcinoma metastasis cell-cell adhesion molecule induce epithelial-mesenchymal transition reduction mammoplasty tissue anti-human catenin-β tumour necrosis factor-α caf-conditioned medium induces e-cadherin mediates adhesion caf-conditioned medium exhibited cell-adhesion-dependent influences nmf-conditioned media enhances nmf-conditioned medium exhibited α-smooth muscle actin human breast-cancer cells reactive-tumour stromal cells pmc42-la organoids exhibited pmc42-la monolayers cultured concentrating nmf-conditioned media pmc42-la cell cultures caf-conditioned medium appeared breast fibroblast-conditioned medium smooth-muscle stromal cells smooth muscle-specific proteins full size image concentrated nmf-conditioned media casein/tris-buffered saline pmc42-la monolayer cultures pmc42-la monolayer cells stray pmc42-la cells pmc42-la cell proliferation α-sma-expressing cells vimentin-positive cells layered pmc42-la organoid morphology pmc42-la cells cultured culture pmc42-la cells e-cadherin expression correlates e-cadherin expressing cells epithelial-mesenchymal transition 2-dimensional pmc42-la culture bovine serum albumin/pbs pmc42-la cell wounds

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Induction of epithelial to mesenchymal transition in PMC42-LA human breast carcinoma cells by carcinoma-associated fibroblast secreted factors
         description:Breast carcinoma is accompanied by changes in the acellular and cellular components of the microenvironment, the latter typified by a switch from fibroblasts to myofibroblasts. We utilised conditioned media cultures, Western blot analysis and immunocytochemistry to investigate the differential effects of normal mammary fibroblasts (NMFs) and mammary cancer-associated fibroblasts (CAFs) on the phenotype and behaviour of PMC42-LA breast cancer cells. NMFs were obtained from a mammary gland at reduction mammoplasty, and CAFs from a mammary carcinoma after resection. We found greater expression of myofibroblastic markers in CAFs than in NMFs. Medium from both CAFs and NMFs induced novel expression of α-smooth muscle actin and cytokeratin-14 in PMC42-LA organoids. However, although conditioned media from NMFs resulted in distribution of vimentin-positive cells to the periphery of PMC42-LA organoids, this was not seen with CAF-conditioned medium. Upregulation of vimentin was accompanied by a mis-localization of E-cadherin, suggesting a loss of adhesive function. This was confirmed by visualizing the change in active β-catenin, localized to the cell junctions in control cells/cells in NMF-conditioned medium, to inactive β-catenin, localized to nuclei and cytoplasm in cells in CAF-conditioned medium. We found no significant difference between the influences of NMFs and CAFs on PMC42-LA cell proliferation, viability, or apoptosis; significantly, we demonstrated a role for CAFs, but not for NMFs, in increasing the migratory ability of PMC42-LA cells. By concentrating NMF-conditioned media, we demonstrated the presence of factor(s) that induce epithelial-mesenchymal transition in NMF-conditioned media that are present at higher levels in CAF-conditioned media. Our in vitro results are consistent with observations in vivo showing that alterations in stroma influence the phenotype and behaviour of surrounding cells and provide evidence for a role for CAFs in stimulating cancer progression via an epithelial-mesenchymal transition. These findings have implications for our understanding of the roles of signalling between epithelial and stromal cells in the development and progression of mammary carcinoma.
         datePublished:2007-02-20T00:00:00Z
         dateModified:2007-02-20T00:00:00Z
         pageStart:1
         pageEnd:15
         license:http://creativecommons.org/licenses/by/2.0/
         sameAs:https://doi.org/10.1186/bcr1656
         keywords:
            Conditioned Medium
            Vimentin Expression
            Fibroblast Activation Protein
            Reduction Mammoplasty
            Myoepithelial Marker
            Cancer Research
            Oncology
            Surgical Oncology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig1_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig2_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig3_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig4_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig5_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig6_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig7_HTML.jpg
         isPartOf:
            name:Breast Cancer Research
            issn:
               1465-542X
            volumeNumber:9
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Stephanie C Lebret
               affiliation:
                     name:Deakin University
                     address:
                        name:Deakin University, Burwood, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Donald F Newgreen
               affiliation:
                     name:The Murdoch Children's Research Institute
                     address:
                        name:The Murdoch Children's Research Institute, Parkville, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Erik W Thompson
               affiliation:
                     name:University of Melbourne
                     address:
                        name:Department of Surgery, University of Melbourne, Parkville, Australia
                        type:PostalAddress
                     type:Organization
                     name:Victoria Parade
                     address:
                        name:St. Vincent's Institute of Medical Research, Victoria Parade, Fitzroy, Australia
                        type:PostalAddress
                     type:Organization
                     name:Bernard O'Brien Institute for Microsurgery
                     address:
                        name:Bernard O'Brien Institute for Microsurgery, Fitzroy, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:M Leigh Ackland
               affiliation:
                     name:Deakin University
                     address:
                        name:Deakin University, Burwood, Australia
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Induction of epithelial to mesenchymal transition in PMC42-LA human breast carcinoma cells by carcinoma-associated fibroblast secreted factors
      description:Breast carcinoma is accompanied by changes in the acellular and cellular components of the microenvironment, the latter typified by a switch from fibroblasts to myofibroblasts. We utilised conditioned media cultures, Western blot analysis and immunocytochemistry to investigate the differential effects of normal mammary fibroblasts (NMFs) and mammary cancer-associated fibroblasts (CAFs) on the phenotype and behaviour of PMC42-LA breast cancer cells. NMFs were obtained from a mammary gland at reduction mammoplasty, and CAFs from a mammary carcinoma after resection. We found greater expression of myofibroblastic markers in CAFs than in NMFs. Medium from both CAFs and NMFs induced novel expression of α-smooth muscle actin and cytokeratin-14 in PMC42-LA organoids. However, although conditioned media from NMFs resulted in distribution of vimentin-positive cells to the periphery of PMC42-LA organoids, this was not seen with CAF-conditioned medium. Upregulation of vimentin was accompanied by a mis-localization of E-cadherin, suggesting a loss of adhesive function. This was confirmed by visualizing the change in active β-catenin, localized to the cell junctions in control cells/cells in NMF-conditioned medium, to inactive β-catenin, localized to nuclei and cytoplasm in cells in CAF-conditioned medium. We found no significant difference between the influences of NMFs and CAFs on PMC42-LA cell proliferation, viability, or apoptosis; significantly, we demonstrated a role for CAFs, but not for NMFs, in increasing the migratory ability of PMC42-LA cells. By concentrating NMF-conditioned media, we demonstrated the presence of factor(s) that induce epithelial-mesenchymal transition in NMF-conditioned media that are present at higher levels in CAF-conditioned media. Our in vitro results are consistent with observations in vivo showing that alterations in stroma influence the phenotype and behaviour of surrounding cells and provide evidence for a role for CAFs in stimulating cancer progression via an epithelial-mesenchymal transition. These findings have implications for our understanding of the roles of signalling between epithelial and stromal cells in the development and progression of mammary carcinoma.
      datePublished:2007-02-20T00:00:00Z
      dateModified:2007-02-20T00:00:00Z
      pageStart:1
      pageEnd:15
      license:http://creativecommons.org/licenses/by/2.0/
      sameAs:https://doi.org/10.1186/bcr1656
      keywords:
         Conditioned Medium
         Vimentin Expression
         Fibroblast Activation Protein
         Reduction Mammoplasty
         Myoepithelial Marker
         Cancer Research
         Oncology
         Surgical Oncology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig1_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig2_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig3_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig4_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig5_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig6_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fbcr1656/MediaObjects/13058_2006_1621_Fig7_HTML.jpg
      isPartOf:
         name:Breast Cancer Research
         issn:
            1465-542X
         volumeNumber:9
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Stephanie C Lebret
            affiliation:
                  name:Deakin University
                  address:
                     name:Deakin University, Burwood, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Donald F Newgreen
            affiliation:
                  name:The Murdoch Children's Research Institute
                  address:
                     name:The Murdoch Children's Research Institute, Parkville, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Erik W Thompson
            affiliation:
                  name:University of Melbourne
                  address:
                     name:Department of Surgery, University of Melbourne, Parkville, Australia
                     type:PostalAddress
                  type:Organization
                  name:Victoria Parade
                  address:
                     name:St. Vincent's Institute of Medical Research, Victoria Parade, Fitzroy, Australia
                     type:PostalAddress
                  type:Organization
                  name:Bernard O'Brien Institute for Microsurgery
                  address:
                     name:Bernard O'Brien Institute for Microsurgery, Fitzroy, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:M Leigh Ackland
            affiliation:
                  name:Deakin University
                  address:
                     name:Deakin University, Burwood, Australia
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Breast Cancer Research
      issn:
         1465-542X
      volumeNumber:9
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Deakin University
      address:
         name:Deakin University, Burwood, Australia
         type:PostalAddress
      name:The Murdoch Children's Research Institute
      address:
         name:The Murdoch Children's Research Institute, Parkville, Australia
         type:PostalAddress
      name:University of Melbourne
      address:
         name:Department of Surgery, University of Melbourne, Parkville, Australia
         type:PostalAddress
      name:Victoria Parade
      address:
         name:St. Vincent's Institute of Medical Research, Victoria Parade, Fitzroy, Australia
         type:PostalAddress
      name:Bernard O'Brien Institute for Microsurgery
      address:
         name:Bernard O'Brien Institute for Microsurgery, Fitzroy, Australia
         type:PostalAddress
      name:Deakin University
      address:
         name:Deakin University, Burwood, Australia
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Stephanie C Lebret
      affiliation:
            name:Deakin University
            address:
               name:Deakin University, Burwood, Australia
               type:PostalAddress
            type:Organization
      name:Donald F Newgreen
      affiliation:
            name:The Murdoch Children's Research Institute
            address:
               name:The Murdoch Children's Research Institute, Parkville, Australia
               type:PostalAddress
            type:Organization
      name:Erik W Thompson
      affiliation:
            name:University of Melbourne
            address:
               name:Department of Surgery, University of Melbourne, Parkville, Australia
               type:PostalAddress
            type:Organization
            name:Victoria Parade
            address:
               name:St. Vincent's Institute of Medical Research, Victoria Parade, Fitzroy, Australia
               type:PostalAddress
            type:Organization
            name:Bernard O'Brien Institute for Microsurgery
            address:
               name:Bernard O'Brien Institute for Microsurgery, Fitzroy, Australia
               type:PostalAddress
            type:Organization
      name:M Leigh Ackland
      affiliation:
            name:Deakin University
            address:
               name:Deakin University, Burwood, Australia
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Deakin University, Burwood, Australia
      name:The Murdoch Children's Research Institute, Parkville, Australia
      name:Department of Surgery, University of Melbourne, Parkville, Australia
      name:St. Vincent's Institute of Medical Research, Victoria Parade, Fitzroy, Australia
      name:Bernard O'Brien Institute for Microsurgery, Fitzroy, Australia
      name:Deakin University, Burwood, Australia

External Links {🔗}(224)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

5.13s.

LINK . SPRINGER . COM {}