We are analyzing https://link.springer.com/article/10.1186/ar4239.

Title:
A genetic role for macrophage migration inhibitory factor (MIF) in adult-onset Still's disease | Arthritis Research & Therapy
Description:
Introduction Adult-onset still
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

mif, aosd, patients, disease, article, pubmed, factor, macrophage, gene, google, scholar, migration, inhibitory, catt, expression, promoter, controls, cas, dna, polymorphisms, arthritis, levels, stills, study, plasma, genotype, clinical, pcr, data, role, inflammatory, methylation, allele, polymorphism, frequencies, compared, haplotype, association, table, snp, functional, minutes, severity, higher, adult, analysis, repeat, reverse, frequency, primer,

Topics {✒️}

reverse primer 5'-ggg-gca-cgt-tgg-tgt-tta-c-3' forward primer 5'-act-aag-aaa-gac-ccg-agg-c-3' 5'-tcc-act-aat-ggt-aaa-ctc-g-3' 5'-ttg-cac-cta-tca-gag-acc-3' parametric mann-whitney u-test monocyte/macrophage-derived inflammatory cytokines anti-double-stranded dna antibody pcr-amplified bisulfate-treated dna monocyte/macrophage-derived mediators tumor necrosis factor-α liang-jing lu ficoll-hypaque gradient centrifugation article download pdf real-time reverse transcriptase full size image related subjects real-time reverse transcription anti-tnfalpha therapy taq dna polymerase clinical syndrome single nucleotide polymorphism privacy choices/manage cookies 366-bp dna fragment functional promoter polymorphism human leukocyte antigen mif gene polymorphisms full access diaz-gonzalez ja systemic inflammatory disorder gastrointestinal cancer cells article wang real-time pcr mif promoter polymorphisms interleukin-18 gene polymorphisms article number r65 glyceraldehyde-3-phosphate dehydrogenase catt tetranucleotide repeat systemic lupus erythematosus reverse transcription kit plasma mif levels mif plasma levels influences gene expression mif gene promoter functional mif polymorphisms shanghai jiaotong university yale university school lópez-nevot ma lopez-nevot ma gonzález-gay ma mif gene expression

Questions {❓}

Feldmann M, Brennan FM, Paleolog E, Cope A, Taylor P, Williams R, Woody J, Maini RN: Anti-TNFalpha therapy of rheumatoid arthritis: what can we learn about chronic disease?

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WebPage:
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         headline:A genetic role for macrophage migration inhibitory factor (MIF) in adult-onset Still's disease
         description:Adult-onset still's disease (AOSD) is a rare systemic inflammatory disorder in which abnormalities in inflammatory cytokines production appear to play a pathophysiological role. Our previous work has reported increased expression of macrophage migration inhibitory factor (MIF) and revealed its correlation with disease severity and activity in AOSD. A -173 G/C single nucleotide polymorphism (SNP) (rs755622) and a -794 CATT5-8 repeat (rs5844572) in the MIF promoter have been reported. In this study, we sought to explore the relationship between functional MIF promoter polymorphisms and MIF expression in AOSD. 100 patients and 200 controls were recruited in the study. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was utilized to analyze the -173 G/C SNP (rs755622) and PCR-based size discrimination assay was applied to detect the -794 CATT5-8 repeat (rs5844572) in the MIF promoter. Plasma MIF levels were measured by ELISA. MIF mRNA levels were quantified by real-time reverse transcription (RT)-PCR. Bisulfate genomic sequencing was employed to evaluate DNA methylation status within the MIF promoter. We identified that the frequencies of MIF -794 CATT5 (P = 0.001) allele and the expression of MIF (P <0.001) were increased in patients compared to healthy controls. Plasma levels of MIF in patients with CC genotype were higher than those of patients with GC or GG genotypes (P = 0.05). In patients with established AOSD, a higher frequency of -794 CATT7 containing MIF genotypes was observed in those with liver dysfunction (P = 0.009). Haplotype analysis revealed a higher representation of the MIF haplotype defined by -173*C/-794 CATT5 (C5) in AOSD patients (P = 0.001). Functional promoter polymorphisms in the MIF gene influence plasma MIF levels in AOSD and may contribute to disease susceptibility or clinical presentation of AOSD.
         datePublished:2013-10-17T00:00:00Z
         dateModified:2013-10-17T00:00:00Z
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            adult onset still's disease
            gene polymorphism
            gene expression
            DNA methylation
            Rheumatology
            Orthopedics
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      headline:A genetic role for macrophage migration inhibitory factor (MIF) in adult-onset Still's disease
      description:Adult-onset still's disease (AOSD) is a rare systemic inflammatory disorder in which abnormalities in inflammatory cytokines production appear to play a pathophysiological role. Our previous work has reported increased expression of macrophage migration inhibitory factor (MIF) and revealed its correlation with disease severity and activity in AOSD. A -173 G/C single nucleotide polymorphism (SNP) (rs755622) and a -794 CATT5-8 repeat (rs5844572) in the MIF promoter have been reported. In this study, we sought to explore the relationship between functional MIF promoter polymorphisms and MIF expression in AOSD. 100 patients and 200 controls were recruited in the study. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was utilized to analyze the -173 G/C SNP (rs755622) and PCR-based size discrimination assay was applied to detect the -794 CATT5-8 repeat (rs5844572) in the MIF promoter. Plasma MIF levels were measured by ELISA. MIF mRNA levels were quantified by real-time reverse transcription (RT)-PCR. Bisulfate genomic sequencing was employed to evaluate DNA methylation status within the MIF promoter. We identified that the frequencies of MIF -794 CATT5 (P = 0.001) allele and the expression of MIF (P <0.001) were increased in patients compared to healthy controls. Plasma levels of MIF in patients with CC genotype were higher than those of patients with GC or GG genotypes (P = 0.05). In patients with established AOSD, a higher frequency of -794 CATT7 containing MIF genotypes was observed in those with liver dysfunction (P = 0.009). Haplotype analysis revealed a higher representation of the MIF haplotype defined by -173*C/-794 CATT5 (C5) in AOSD patients (P = 0.001). Functional promoter polymorphisms in the MIF gene influence plasma MIF levels in AOSD and may contribute to disease susceptibility or clinical presentation of AOSD.
      datePublished:2013-10-17T00:00:00Z
      dateModified:2013-10-17T00:00:00Z
      pageStart:1
      pageEnd:8
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         macrophage migration inhibitory factor
         adult onset still's disease
         gene polymorphism
         gene expression
         DNA methylation
         Rheumatology
         Orthopedics
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      name:Department of Medicine, Section of Rheumatology, Yale University School of Medicine, The Anlyan Center, New Haven, USA
      name:Department of Medicine, Section of Rheumatology, Yale University School of Medicine, The Anlyan Center, New Haven, USA
      name:Department of Rheumatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
      name:Department of Rheumatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China

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