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We are analyzing https://link.springer.com/article/10.1186/ar407.

Title:
Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue | Arthritis Research & Therapy
Description:
Angiopoietins (Ang) are vascular endothelial cell-specific growth factors that play important roles principally during the later stages of angiogenesis. We have compared the distribution of the receptor tyrosine kinase (Tie) and the Ang ligands in synovial tissues from normal subjects and those with rheumatoid arthritis (RA) and osteoarthritis (OA). Immunohistochemical analysis was used to determine the expression of Ang-1, Ang-2, Tie1 and Tie2 in synovial tissue of normal subjects and those with RA and OA. Ang-1, Ang-2, Tie1 and Tie2 mRNA and protein expression were quantified in synovial tissues and RA synovial tissue fibroblasts with real-time reverse transcription polymerase chain reaction and western blot analysis. In RA, Ang-1 positive immunostaining on lining cells, macrophages and endothelial cells was significantly higher than in OA and normal synovial tissue. The expression pattern of Ang-2 in synovial tissue was similar in RA and OA, whereas the Ang-2 expression was low in normal tissue. Synovial tissue from subjects with RA and OA showed a significant upregulation of Tie1 on lining cells, macrophages and endothelial cells compared to that from normal subjects. Tie2 was significantly upregulated in the RA and OA synovial tissue lining cells, macrophages and smooth muscle cells compared to normal synovial tissue. Generally Ang-1, Ang-2, Tie1 and Tie2 mRNA levels were higher in RA synovial tissue compared to normal and OA synovial tissues, and RA synovial tissue fibroblasts. Western blot analysis also demonstrated greater Tie1 and Tie2 protein expression in RA and OA synovial tissue compared to RA synovial tissue fibroblasts. In conclusion, the dominance of Ang-1 mRNA and protein expression over Ang-2 is in agreement with an active neovascularization in RA synovial tissue.
Website Age:
28 years and 1 months (reg. 1997-05-29).

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Custom-built

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🌠 Phenomenal Traffic: 5M - 10M visitors per month


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How Does Link.springer.com Make Money? {πŸ’Έ}

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Keywords {πŸ”}

synovial, tie, ang, tissue, cells, expression, normal, endothelial, article, usa, vascular, arthritis, macrophages, cas, google, scholar, mrna, fibroblasts, tissues, lining, staining, receptor, cell, rheumatoid, compared, analysis, muscle, osteoarthritis, protein, positive, higher, smooth, santa, cruz, endothelium, reverse, vegf, angiogenesis, subjects, angiopoietin, antibody, significantly, koch, growth, western, patients, immunohistochemistry, antihuman, angiogenic, blot,

Topics {βœ’οΈ}

real-time reverse transcription-pcr real-time pcr quantification tumor necrosis factor-Ξ± semi-dry transblotting apparatus article download pdf secretion-trap expression cloning tgf-Ξ² receptor endoglin horseradish-peroxidase-conjugated antibody tris-buffered saline tween superscript ii rt antagonizing ang-1-induced autophosphorylation arrows denote administration research service receptor tyrosine kinase full size image rt-pcr analysis confirmed growth factor stimulators full access blood vessel formation smooth muscle cells machine learning reverse transcription efficiency rheumatoid synovial tissue synovial tissue homogenization lining cell layer synovial tissue components privacy choices/manage cookies ligand binding domain arthritis research goat antihuman antibody isotype-specific igg acid phenol method rat adjuvant arthritis rabbit antihuman tie1 human endothelial cells demonstrated greater tie1 human synovial fluids optimal cutting temperature thermal cycling conditions cell components examined tie receptor family embryonic lethality due western blot analysis stimulate corneal neovascularization bicinchoninic acid assay goat antihuman ang-1 soluble tie receptor reverse transcribed simultaneously rheumatoid arthritis cell types compared

Schema {πŸ—ΊοΈ}

WebPage:
      mainEntity:
         headline:Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue
         description:Angiopoietins (Ang) are vascular endothelial cell-specific growth factors that play important roles principally during the later stages of angiogenesis. We have compared the distribution of the receptor tyrosine kinase (Tie) and the Ang ligands in synovial tissues from normal subjects and those with rheumatoid arthritis (RA) and osteoarthritis (OA). Immunohistochemical analysis was used to determine the expression of Ang-1, Ang-2, Tie1 and Tie2 in synovial tissue of normal subjects and those with RA and OA. Ang-1, Ang-2, Tie1 and Tie2 mRNA and protein expression were quantified in synovial tissues and RA synovial tissue fibroblasts with real-time reverse transcription polymerase chain reaction and western blot analysis. In RA, Ang-1 positive immunostaining on lining cells, macrophages and endothelial cells was significantly higher than in OA and normal synovial tissue. The expression pattern of Ang-2 in synovial tissue was similar in RA and OA, whereas the Ang-2 expression was low in normal tissue. Synovial tissue from subjects with RA and OA showed a significant upregulation of Tie1 on lining cells, macrophages and endothelial cells compared to that from normal subjects. Tie2 was significantly upregulated in the RA and OA synovial tissue lining cells, macrophages and smooth muscle cells compared to normal synovial tissue. Generally Ang-1, Ang-2, Tie1 and Tie2 mRNA levels were higher in RA synovial tissue compared to normal and OA synovial tissues, and RA synovial tissue fibroblasts. Western blot analysis also demonstrated greater Tie1 and Tie2 protein expression in RA and OA synovial tissue compared to RA synovial tissue fibroblasts. In conclusion, the dominance of Ang-1 mRNA and protein expression over Ang-2 is in agreement with an active neovascularization in RA synovial tissue.
         datePublished:2002-04-01T00:00:00Z
         dateModified:2002-04-01T00:00:00Z
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            mRNA
            rheumatoid arthritis
            Tie receptor expression
            Rheumatology
            Orthopedics
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      headline:Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue
      description:Angiopoietins (Ang) are vascular endothelial cell-specific growth factors that play important roles principally during the later stages of angiogenesis. We have compared the distribution of the receptor tyrosine kinase (Tie) and the Ang ligands in synovial tissues from normal subjects and those with rheumatoid arthritis (RA) and osteoarthritis (OA). Immunohistochemical analysis was used to determine the expression of Ang-1, Ang-2, Tie1 and Tie2 in synovial tissue of normal subjects and those with RA and OA. Ang-1, Ang-2, Tie1 and Tie2 mRNA and protein expression were quantified in synovial tissues and RA synovial tissue fibroblasts with real-time reverse transcription polymerase chain reaction and western blot analysis. In RA, Ang-1 positive immunostaining on lining cells, macrophages and endothelial cells was significantly higher than in OA and normal synovial tissue. The expression pattern of Ang-2 in synovial tissue was similar in RA and OA, whereas the Ang-2 expression was low in normal tissue. Synovial tissue from subjects with RA and OA showed a significant upregulation of Tie1 on lining cells, macrophages and endothelial cells compared to that from normal subjects. Tie2 was significantly upregulated in the RA and OA synovial tissue lining cells, macrophages and smooth muscle cells compared to normal synovial tissue. Generally Ang-1, Ang-2, Tie1 and Tie2 mRNA levels were higher in RA synovial tissue compared to normal and OA synovial tissues, and RA synovial tissue fibroblasts. Western blot analysis also demonstrated greater Tie1 and Tie2 protein expression in RA and OA synovial tissue compared to RA synovial tissue fibroblasts. In conclusion, the dominance of Ang-1 mRNA and protein expression over Ang-2 is in agreement with an active neovascularization in RA synovial tissue.
      datePublished:2002-04-01T00:00:00Z
      dateModified:2002-04-01T00:00:00Z
      pageStart:1
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      sameAs:https://doi.org/10.1186/ar407
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         angiopoietin
         mRNA
         rheumatoid arthritis
         Tie receptor expression
         Rheumatology
         Orthopedics
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               type:PostalAddress
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               name:Department of Pathology, Northwestern University Medical School, USA
               type:PostalAddress
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      name:Alisa E Koch
      affiliation:
            name:Northwestern University Medical School
            address:
               name:Department of Medicine, Northwestern University Medical School, USA
               type:PostalAddress
            type:Organization
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      name:Department of Medicine, Northwestern University Medical School, USA
      name:Department of Pathology, Northwestern University Medical School, USA
      name:Department of Medicine, Northwestern University Medical School, USA
      name:Veterans Administration, Chicago Health Care System, Lakeside Division, Chicago, USA

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