We are analyzing https://link.springer.com/article/10.1186/1756-0500-4-247.

Title:
Dendritic Cells Activate and Mature after Infection with Mycobacterium tuberculosis | BMC Research Notes
Description:
Background Dendritic cells (DCs) can take up an array of different antigens, including microorganisms which they can process and present more effectively than any other antigen presenting cell. However, whether the interaction between the human DC and Mycobacterium tuberculosis represents a defense mechanism by the invaded host, or helping the invader to evade the defense mechanism of the host is still not clearly understood. Findings To analyze the interactions between M. tuberculosis and immune cells, human peripheral blood monocyte-derived immature DCs were infected with M. tuberculosis H37Rv wild type strain and flow cytometry was used to analyse cell surface expression markers. The ability of the M. tuberculosis infected DC to induce T cell proliferation using 5 and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution technique was also investigated. DCs were found to internalize the mycobacteria and show dose dependent infection and necrosis with different multiplicity of infection. Flow cytometry analysis of cell surface expression markers CD40, CD54, CD80, CD83, CD86 and HLA DR in infected DC revealed significant (p < 0.05) up regulation following infection with M. tuberculosis in comparison to immature DC with no stimulation. Lipopolysaccharide (LPS) from Salmonella abortus equi, a known DC maturation agent, was used as a positive control and showed a comparable up regulation of cell surface markers as observed with M. tuberculosis infected DC. It was revealed that the M. tuberculosis infected DC induced T cell proliferation. Conclusion These data clearly demonstrate that M. tuberculosis induces activation and maturation of human monocyte-derived immature DC as well as induces T cell proliferation in vitro.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

While many websites aim to make money, others are created to share knowledge or showcase creativity. People build websites for various reasons. This could be one of them. Link.springer.com might be earning cash quietly, but we haven't detected the monetization method.

Keywords {🔍}

cells, cell, tuberculosis, infected, dendritic, infection, article, dcs, human, antigen, surface, maturation, positive, proliferation, pubmed, immature, expression, hrv, mouse, moi, google, scholar, mhc, lps, cas, regulation, molecules, clone, igg, antibody, data, mycobacterium, immune, cfse, response, low, medium, control, composition, ccr, study, cdi, addis, ababa, research, monocytederived, markers, activation, complete, aad,

Topics {✒️}

mixed dc-autologous-t-cell reaction dc-mediated t-cell stimulation rare autoantigen-specific human human monocyte-derived dcs anti-human tnf α t-cell stimulatory capacity article download pdf monocyte-derived immature dc enzyme-linked immunosorbent assay intracellular adhesion molecule medical faculty research intracellular acid-fast bacteria monoclonal antibodies conjugated mycobacteria target dc-sign mixed leukocyte reaction human rgm-csf dendritic cells activate privacy choices/manage cookies mouse igg 2a human dendritic cells stimulatory surface molecules t-cell response middlebrook 7h10 agar immature human dc cd40 fitc/cd80 pe mhc class ii orchestrate signals derived measles virus [11] full access authors’ original file cd83 fitc/cd86 pe surface marker expression mycobacterium tuberculosis represents acid-fast bacteria cfse dilution technique tuberculosis strain leads mixed dc-autologous stimulatory molecules cd40 biomed central cell surface markers murine dendritic cells hla dr fitc article mihret virulent mycobacterium tuberculosis subsequently displayed phenotypic mentioned surface molecules tuberculosis induces activation cfsebright cd3+ cells cfse dilution techniques tuberculosis occurred annually

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Dendritic Cells Activate and Mature after Infection with Mycobacterium tuberculosis
         description:Dendritic cells (DCs) can take up an array of different antigens, including microorganisms which they can process and present more effectively than any other antigen presenting cell. However, whether the interaction between the human DC and Mycobacterium tuberculosis represents a defense mechanism by the invaded host, or helping the invader to evade the defense mechanism of the host is still not clearly understood. To analyze the interactions between M. tuberculosis and immune cells, human peripheral blood monocyte-derived immature DCs were infected with M. tuberculosis H37Rv wild type strain and flow cytometry was used to analyse cell surface expression markers. The ability of the M. tuberculosis infected DC to induce T cell proliferation using 5 and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution technique was also investigated. DCs were found to internalize the mycobacteria and show dose dependent infection and necrosis with different multiplicity of infection. Flow cytometry analysis of cell surface expression markers CD40, CD54, CD80, CD83, CD86 and HLA DR in infected DC revealed significant (p < 0.05) up regulation following infection with M. tuberculosis in comparison to immature DC with no stimulation. Lipopolysaccharide (LPS) from Salmonella abortus equi, a known DC maturation agent, was used as a positive control and showed a comparable up regulation of cell surface markers as observed with M. tuberculosis infected DC. It was revealed that the M. tuberculosis infected DC induced T cell proliferation. These data clearly demonstrate that M. tuberculosis induces activation and maturation of human monocyte-derived immature DC as well as induces T cell proliferation in vitro.
         datePublished:2011-07-21T00:00:00Z
         dateModified:2011-07-21T00:00:00Z
         pageStart:1
         pageEnd:7
         license:http://creativecommons.org/licenses/by/2.0
         sameAs:https://doi.org/10.1186/1756-0500-4-247
         keywords:
            Dendritic cells
            Mycobacterium tuberculosis
            T cells
            Activation
            Flowctometry
            CFSE
            Proliferation
            Biomedicine
            general
            Medicine/Public Health
            Life Sciences
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1756-0500-4-247/MediaObjects/13104_2011_Article_981_Fig1_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1756-0500-4-247/MediaObjects/13104_2011_Article_981_Fig2_HTML.jpg
         isPartOf:
            name:BMC Research Notes
            issn:
               1756-0500
            volumeNumber:4
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Adane Mihret
               affiliation:
                     name:Armauer Hansen Research Institute (AHRI)
                     address:
                        name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
                        type:PostalAddress
                     type:Organization
                     name:Addis Ababa University
                     address:
                        name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Gezahagne Mamo
               affiliation:
                     name:Armauer Hansen Research Institute (AHRI)
                     address:
                        name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
                        type:PostalAddress
                     type:Organization
                     name:Addis Ababa, University
                     address:
                        name:Faculty of Veterinary Medicine, Addis Ababa, University, Addis Ababa, Ethiopia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Mesfin Tafesse
               affiliation:
                     name:Armauer Hansen Research Institute (AHRI)
                     address:
                        name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Asrat Hailu
               affiliation:
                     name:Addis Ababa University
                     address:
                        name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Shreemanta Parida
               affiliation:
                     name:Armauer Hansen Research Institute (AHRI)
                     address:
                        name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
                        type:PostalAddress
                     type:Organization
                     name:Max Planck Institute for Infection Biology
                     address:
                        name:Max Planck Institute for Infection Biology, Berlin, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Dendritic Cells Activate and Mature after Infection with Mycobacterium tuberculosis
      description:Dendritic cells (DCs) can take up an array of different antigens, including microorganisms which they can process and present more effectively than any other antigen presenting cell. However, whether the interaction between the human DC and Mycobacterium tuberculosis represents a defense mechanism by the invaded host, or helping the invader to evade the defense mechanism of the host is still not clearly understood. To analyze the interactions between M. tuberculosis and immune cells, human peripheral blood monocyte-derived immature DCs were infected with M. tuberculosis H37Rv wild type strain and flow cytometry was used to analyse cell surface expression markers. The ability of the M. tuberculosis infected DC to induce T cell proliferation using 5 and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution technique was also investigated. DCs were found to internalize the mycobacteria and show dose dependent infection and necrosis with different multiplicity of infection. Flow cytometry analysis of cell surface expression markers CD40, CD54, CD80, CD83, CD86 and HLA DR in infected DC revealed significant (p < 0.05) up regulation following infection with M. tuberculosis in comparison to immature DC with no stimulation. Lipopolysaccharide (LPS) from Salmonella abortus equi, a known DC maturation agent, was used as a positive control and showed a comparable up regulation of cell surface markers as observed with M. tuberculosis infected DC. It was revealed that the M. tuberculosis infected DC induced T cell proliferation. These data clearly demonstrate that M. tuberculosis induces activation and maturation of human monocyte-derived immature DC as well as induces T cell proliferation in vitro.
      datePublished:2011-07-21T00:00:00Z
      dateModified:2011-07-21T00:00:00Z
      pageStart:1
      pageEnd:7
      license:http://creativecommons.org/licenses/by/2.0
      sameAs:https://doi.org/10.1186/1756-0500-4-247
      keywords:
         Dendritic cells
         Mycobacterium tuberculosis
         T cells
         Activation
         Flowctometry
         CFSE
         Proliferation
         Biomedicine
         general
         Medicine/Public Health
         Life Sciences
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1756-0500-4-247/MediaObjects/13104_2011_Article_981_Fig1_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1756-0500-4-247/MediaObjects/13104_2011_Article_981_Fig2_HTML.jpg
      isPartOf:
         name:BMC Research Notes
         issn:
            1756-0500
         volumeNumber:4
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Adane Mihret
            affiliation:
                  name:Armauer Hansen Research Institute (AHRI)
                  address:
                     name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
                     type:PostalAddress
                  type:Organization
                  name:Addis Ababa University
                  address:
                     name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Gezahagne Mamo
            affiliation:
                  name:Armauer Hansen Research Institute (AHRI)
                  address:
                     name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
                     type:PostalAddress
                  type:Organization
                  name:Addis Ababa, University
                  address:
                     name:Faculty of Veterinary Medicine, Addis Ababa, University, Addis Ababa, Ethiopia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mesfin Tafesse
            affiliation:
                  name:Armauer Hansen Research Institute (AHRI)
                  address:
                     name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Asrat Hailu
            affiliation:
                  name:Addis Ababa University
                  address:
                     name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Shreemanta Parida
            affiliation:
                  name:Armauer Hansen Research Institute (AHRI)
                  address:
                     name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
                     type:PostalAddress
                  type:Organization
                  name:Max Planck Institute for Infection Biology
                  address:
                     name:Max Planck Institute for Infection Biology, Berlin, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:BMC Research Notes
      issn:
         1756-0500
      volumeNumber:4
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Armauer Hansen Research Institute (AHRI)
      address:
         name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
         type:PostalAddress
      name:Addis Ababa University
      address:
         name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
         type:PostalAddress
      name:Armauer Hansen Research Institute (AHRI)
      address:
         name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
         type:PostalAddress
      name:Addis Ababa, University
      address:
         name:Faculty of Veterinary Medicine, Addis Ababa, University, Addis Ababa, Ethiopia
         type:PostalAddress
      name:Armauer Hansen Research Institute (AHRI)
      address:
         name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
         type:PostalAddress
      name:Addis Ababa University
      address:
         name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
         type:PostalAddress
      name:Armauer Hansen Research Institute (AHRI)
      address:
         name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
         type:PostalAddress
      name:Max Planck Institute for Infection Biology
      address:
         name:Max Planck Institute for Infection Biology, Berlin, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Adane Mihret
      affiliation:
            name:Armauer Hansen Research Institute (AHRI)
            address:
               name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
               type:PostalAddress
            type:Organization
            name:Addis Ababa University
            address:
               name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Gezahagne Mamo
      affiliation:
            name:Armauer Hansen Research Institute (AHRI)
            address:
               name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
               type:PostalAddress
            type:Organization
            name:Addis Ababa, University
            address:
               name:Faculty of Veterinary Medicine, Addis Ababa, University, Addis Ababa, Ethiopia
               type:PostalAddress
            type:Organization
      name:Mesfin Tafesse
      affiliation:
            name:Armauer Hansen Research Institute (AHRI)
            address:
               name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
               type:PostalAddress
            type:Organization
      name:Asrat Hailu
      affiliation:
            name:Addis Ababa University
            address:
               name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
               type:PostalAddress
            type:Organization
      name:Shreemanta Parida
      affiliation:
            name:Armauer Hansen Research Institute (AHRI)
            address:
               name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
               type:PostalAddress
            type:Organization
            name:Max Planck Institute for Infection Biology
            address:
               name:Max Planck Institute for Infection Biology, Berlin, Germany
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
      name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
      name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
      name:Faculty of Veterinary Medicine, Addis Ababa, University, Addis Ababa, Ethiopia
      name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
      name:Faculty of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
      name:Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia
      name:Max Planck Institute for Infection Biology, Berlin, Germany

External Links {🔗}(82)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.94s.

LINK . SPRINGER . COM {}