We are analyzing https://link.springer.com/article/10.1186/1746-6148-8-104.

Title:
Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus | BMC Veterinary Research
Description:
Background Since 2008, a progressive pneumonia has become prevalent in broilers and laying hens. This disease occurrs the first day after hatching and lasts more than 30 days, resulting in approximately 70% morbidity and 30% mortality in broilers. The objective of this study was to isolate and identify the pathogens that are responsible for the progressive pneumonia and establish an animal model for drug screening. Results 193 serum samples were collected from 8 intensive farms from 5 provinces in China and analysed in the current research. Our clinical survey showed that 65.2% to 100% of breeding broilers, breeding layers, broilers and laying hens were seropositive for ORT antibodies. From 8 intensive farms, six ORT isolates were identified by PCR and biochemical assays, and two H9N2 viruses were isolated. Newcastle Disease Virus (NDV) and Infectious BronchitisVirus (IBV) were excluded. Typical pneumonia and airsacculitis were observed both in broilers inoculated intraperitoneally with an ORT isolate alone and in those co-infected with ORT and H9N2 virus isolates. Specifically, the survival rate was 30%, 20%, 70%, 50% and 90% in birds inoculated with ORT+H9N2 virus, ORT followed by H9N2 virus, H9N2 virus followed by ORT, and ORT or H9N2 virus alone, respectively. Conclusions The results of this study suggest that ORT infections of domestic poultry have been occurring frequently in China. ORT infection can induce higher economic losses and mortality if H9N2 AIV is also present. Although the isolation of ORT and H9N2 virus has been reported previously, there have been no reported co-infections of poultry with these two pathogens. This is the first report of co-infection of broilers with ORT and H9N2 virus, and this co-infection is probably associated with the outbreak of broiler airsacculitis in China, which has caused extensive economic losses.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Animals & Wildlife

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't tell how the site generates income.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

ort, virus, broilers, article, google, scholar, infection, ornithobacterium, avian, ortchickenshandong, respiratory, rhinotracheale, birds, group, isolated, china, mortality, study, chickens, table, pneumonia, coinfection, hnchickenshandong, cas, pubmed, influenza, samples, inoculated, poultry, days, isolate, identified, infected, van, research, disease, isolates, pcr, airsacculitis, isolation, broiler, positive, clinical, negative, sequence, eld, vet, authors, including, veterinary,

Topics {✒️}

ort/chicken/shandong/2011 colony produced open access article multi-causal respiratory diseases article download pdf 10 ld50of ort/chicken/shandong/2011 named ort/chicken/shandong/2011 ort/chicken/shandong/2011 showing h9n2/chicken/shandong/2011 intranasally pleomorphic gram-negative rod h9n2/species/location/time ort/chicken/shandong/2011 showed ort/chicken/shandong/2011 strain ort/chicken/shandong/2011 intraperitoneally ort/species/location/time full access privacy choices/manage cookies h9n2/chicken/shandong/2011 multifactorial respiratory diseases commercial testing kit dneasy tissue kit infectious bronchitis virus avian h9n2 influenza authors’ original file avian influenza virus ort+h9n2 virus group ort/h9n2 virus group negative control colony formation units negative pressure isolators ort/chicken/shandong/2011 ort serum-positive broilers sterile physiological saline overt respiratory symptoms infectious bronchitis viruses h9n2 virus isolates positive h9n2 isolates virulent chinese isolates van den bosch ornithobacterium rhinotracheale gen matching serum pairs single colony purification h9n2 virus resulted ornithobacterium rhinotracheale strains h9n2 virus isolate induce clinical signs author information authors clinical survey showed broiler chicken flocks breeding species seroconverted related subjects

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus
         description:Since 2008, a progressive pneumonia has become prevalent in broilers and laying hens. This disease occurrs the first day after hatching and lasts more than 30 days, resulting in approximately 70% morbidity and 30% mortality in broilers. The objective of this study was to isolate and identify the pathogens that are responsible for the progressive pneumonia and establish an animal model for drug screening. 193 serum samples were collected from 8 intensive farms from 5 provinces in China and analysed in the current research. Our clinical survey showed that 65.2% to 100% of breeding broilers, breeding layers, broilers and laying hens were seropositive for ORT antibodies. From 8 intensive farms, six ORT isolates were identified by PCR and biochemical assays, and two H9N2 viruses were isolated. Newcastle Disease Virus (NDV) and Infectious BronchitisVirus (IBV) were excluded. Typical pneumonia and airsacculitis were observed both in broilers inoculated intraperitoneally with an ORT isolate alone and in those co-infected with ORT and H9N2 virus isolates. Specifically, the survival rate was 30%, 20%, 70%, 50% and 90% in birds inoculated with ORT+H9N2 virus, ORT followed by H9N2 virus, H9N2 virus followed by ORT, and ORT or H9N2 virus alone, respectively. The results of this study suggest that ORT infections of domestic poultry have been occurring frequently in China. ORT infection can induce higher economic losses and mortality if H9N2 AIV is also present. Although the isolation of ORT and H9N2 virus has been reported previously, there have been no reported co-infections of poultry with these two pathogens. This is the first report of co-infection of broilers with ORT and H9N2 virus, and this co-infection is probably associated with the outbreak of broiler airsacculitis in China, which has caused extensive economic losses.
         datePublished:2012-07-02T00:00:00Z
         dateModified:2012-07-02T00:00:00Z
         pageStart:1
         pageEnd:7
         license:https://creativecommons.org/licenses/by/2.0
         sameAs:https://doi.org/10.1186/1746-6148-8-104
         keywords:
            Isolation
            Characterisation
             Ornithobacterium rhinotracheale
            H9N2
            Co-infect
            Broiler
            Veterinary Medicine/Veterinary Science
            Zoology
            Transgenics
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1746-6148-8-104/MediaObjects/12917_2011_Article_389_Fig1_HTML.jpg
         isPartOf:
            name:BMC Veterinary Research
            issn:
               1746-6148
            volumeNumber:8
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Qing Pan
               affiliation:
                     name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                     address:
                        name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Aijing Liu
               affiliation:
                     name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                     address:
                        name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Faming Zhang
               affiliation:
                     name:Beijing Veterinary Biological Manufactory
                     address:
                        name:Beijing Veterinary Biological Manufactory, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yong Ling
               affiliation:
                     name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                     address:
                        name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Changbo Ou
               affiliation:
                     name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                     address:
                        name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Na Hou
               affiliation:
                     name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                     address:
                        name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Cheng He
               affiliation:
                     name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                     address:
                        name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                        type:PostalAddress
                     type:Organization
                     name:China Agricultural University, Yuanmingyuan Xilu, Haidian District
                     address:
                        name:College of Veterinary Medicine, China Agricultural University, Yuanmingyuan Xilu, Haidian District, Beijing, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus
      description:Since 2008, a progressive pneumonia has become prevalent in broilers and laying hens. This disease occurrs the first day after hatching and lasts more than 30 days, resulting in approximately 70% morbidity and 30% mortality in broilers. The objective of this study was to isolate and identify the pathogens that are responsible for the progressive pneumonia and establish an animal model for drug screening. 193 serum samples were collected from 8 intensive farms from 5 provinces in China and analysed in the current research. Our clinical survey showed that 65.2% to 100% of breeding broilers, breeding layers, broilers and laying hens were seropositive for ORT antibodies. From 8 intensive farms, six ORT isolates were identified by PCR and biochemical assays, and two H9N2 viruses were isolated. Newcastle Disease Virus (NDV) and Infectious BronchitisVirus (IBV) were excluded. Typical pneumonia and airsacculitis were observed both in broilers inoculated intraperitoneally with an ORT isolate alone and in those co-infected with ORT and H9N2 virus isolates. Specifically, the survival rate was 30%, 20%, 70%, 50% and 90% in birds inoculated with ORT+H9N2 virus, ORT followed by H9N2 virus, H9N2 virus followed by ORT, and ORT or H9N2 virus alone, respectively. The results of this study suggest that ORT infections of domestic poultry have been occurring frequently in China. ORT infection can induce higher economic losses and mortality if H9N2 AIV is also present. Although the isolation of ORT and H9N2 virus has been reported previously, there have been no reported co-infections of poultry with these two pathogens. This is the first report of co-infection of broilers with ORT and H9N2 virus, and this co-infection is probably associated with the outbreak of broiler airsacculitis in China, which has caused extensive economic losses.
      datePublished:2012-07-02T00:00:00Z
      dateModified:2012-07-02T00:00:00Z
      pageStart:1
      pageEnd:7
      license:https://creativecommons.org/licenses/by/2.0
      sameAs:https://doi.org/10.1186/1746-6148-8-104
      keywords:
         Isolation
         Characterisation
          Ornithobacterium rhinotracheale
         H9N2
         Co-infect
         Broiler
         Veterinary Medicine/Veterinary Science
         Zoology
         Transgenics
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1746-6148-8-104/MediaObjects/12917_2011_Article_389_Fig1_HTML.jpg
      isPartOf:
         name:BMC Veterinary Research
         issn:
            1746-6148
         volumeNumber:8
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Qing Pan
            affiliation:
                  name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                  address:
                     name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Aijing Liu
            affiliation:
                  name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                  address:
                     name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Faming Zhang
            affiliation:
                  name:Beijing Veterinary Biological Manufactory
                  address:
                     name:Beijing Veterinary Biological Manufactory, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yong Ling
            affiliation:
                  name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                  address:
                     name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Changbo Ou
            affiliation:
                  name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                  address:
                     name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Na Hou
            affiliation:
                  name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                  address:
                     name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Cheng He
            affiliation:
                  name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
                  address:
                     name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
                     type:PostalAddress
                  type:Organization
                  name:China Agricultural University, Yuanmingyuan Xilu, Haidian District
                  address:
                     name:College of Veterinary Medicine, China Agricultural University, Yuanmingyuan Xilu, Haidian District, Beijing, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:BMC Veterinary Research
      issn:
         1746-6148
      volumeNumber:8
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
      address:
         name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
         type:PostalAddress
      name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
      address:
         name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
         type:PostalAddress
      name:Beijing Veterinary Biological Manufactory
      address:
         name:Beijing Veterinary Biological Manufactory, Beijing, China
         type:PostalAddress
      name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
      address:
         name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
         type:PostalAddress
      name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
      address:
         name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
         type:PostalAddress
      name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
      address:
         name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
         type:PostalAddress
      name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
      address:
         name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
         type:PostalAddress
      name:China Agricultural University, Yuanmingyuan Xilu, Haidian District
      address:
         name:College of Veterinary Medicine, China Agricultural University, Yuanmingyuan Xilu, Haidian District, Beijing, China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Qing Pan
      affiliation:
            name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
            address:
               name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Aijing Liu
      affiliation:
            name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
            address:
               name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Faming Zhang
      affiliation:
            name:Beijing Veterinary Biological Manufactory
            address:
               name:Beijing Veterinary Biological Manufactory, Beijing, China
               type:PostalAddress
            type:Organization
      name:Yong Ling
      affiliation:
            name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
            address:
               name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Changbo Ou
      affiliation:
            name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
            address:
               name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Na Hou
      affiliation:
            name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
            address:
               name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Cheng He
      affiliation:
            name:Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University
            address:
               name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
               type:PostalAddress
            type:Organization
            name:China Agricultural University, Yuanmingyuan Xilu, Haidian District
            address:
               name:College of Veterinary Medicine, China Agricultural University, Yuanmingyuan Xilu, Haidian District, Beijing, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
      name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
      name:Beijing Veterinary Biological Manufactory, Beijing, China
      name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
      name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
      name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
      name:Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
      name:College of Veterinary Medicine, China Agricultural University, Yuanmingyuan Xilu, Haidian District, Beijing, China

External Links {🔗}(95)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Hammer.js
Prism.js

CDN Services {📦}

Crossref

4.87s.

LINK . SPRINGER . COM {}