We are analyzing https://link.springer.com/article/10.1186/1477-5956-9-49.

Title:
Resveratrol suppresses human colon cancer cell proliferation and induces apoptosis via targeting the pentose phosphate and the talin-FAK signaling pathways-A proteomic approach | Proteome Science
Description:
Background We and others have previously reported that resveratrol (RSV) suppresses colon cancer cell proliferation and elevates apoptosis in vitro and/or in vivo, however molecular mechanisms are not fully elucidated. Particularly, little information is available on RSV
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Keywords {🔍}

cancer, cell, rsv, pubmed, google, scholar, igf, cas, cells, colon, pathways, proliferation, resveratrol, apoptosis, signaling, protein, proteins, analysis, fak, pathway, figure, ppp, talin, human, cycle, suppressed, adhesion, growth, progression, high, elevated, suppression, gpdh, data, role, levels, tkt, phosphate, shown, pentose, results, concentrations, proteomic, line, control, presence, file, authors, central, article,

Topics {✒️}

lc/ms/ms-mud pit analysis multi-particulate calcium-pectinate carrier lc/ms/ms-mud pit zinc-pectin-chitosan composite particles igf-1r/akt/wnt signaling pathways igf-1r/akt/mtor signaling pathways igf-1r/wnt signaling pathways integrin-regulated fak-src signaling growth factor-1/igf-1r signaling lc-ms/ms analysis tris-glycine transfer buffer hplc-chip/ms analysis experiment-induced stress response obesity-enhanced colon cancer article download pdf cell-extra cellular matrix pi3-kinase-akt pathways human subjects revealed ultra-ultra mode de la haba vivo colon-specific release pre-formed analysis pipelines lc/ms system consisting target colon cancer biodegradable pectin-based formulation fak integrates growth-factor p53-dependent signaling pathways chemopreventive/anti-proliferative agent igf-1r decreases growth ht-29 cell line triosephosphate isomerase units growth-factor-dependent signals talin-fak signaling pathways produces ribose-5-phosphate required ros-dependent mitochondria pathway immuno-blot pvdf membranes lc/ms/ms talin-fak signaling pathway rsv anti-cancer effects fetal bovine serum cell-ecm interactions mediated colon-specific delivery cancer cell kinetics high concentration-anti-oxidant talin-fak signaling plays bovine serum albumin resveratrol suppresses insulin colorectal cancer patients biological pathway analysis cancer cell proliferation

Questions {❓}

Boros LG, Lee PW, Brandes JL, Cascante M, Muscarella P, Schirmer WJ, Melvin WS, Ellison EC: Nonoxidative pentose phosphate pathways and their direct role in ribose synthesis in tumors: is cancer a disease of cellular glucose metabolism?

Schema {🗺️}

WebPage:
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         headline:Resveratrol suppresses human colon cancer cell proliferation and induces apoptosis via targeting the pentose phosphate and the talin-FAK signaling pathways-A proteomic approach
         description:We and others have previously reported that resveratrol (RSV) suppresses colon cancer cell proliferation and elevates apoptosis in vitro and/or in vivo, however molecular mechanisms are not fully elucidated. Particularly, little information is available on RSV's effects on metabolic pathways and the cell-extra cellular matrix (ECM) communication that are critical for cancer cell growth. To identify important targets of RSV, we analyzed whole protein fractions from HT-29 advanced human colon cancer cell line treated with solvent control, IGF-1 (10 nM) and RSV (150 μM) using LC/MS/MS-Mud PIT (Multidimensional Protein Identification Technology). Pentose phosphate pathway (PPP), a vital metabolic pathway for cell cycle progression, was elevated and suppressed by IGF-1 and RSV, respectively in the HT-29 cell line. Enzymatic assays confirmed RSV suppression of glucose-6 phosphate dehydrogenase (rate limiting) and transketolase, key enzymes of the PPP. RSV (150 μM) suppressed, whereas IGF-1 (10 nM) elevated focal adhesion complex (FAC) proteins, talin and pFAK, critical for the cell-ECM communication. Western blotting analyses confirmed the suppression or elevation of these proteins in HT-29 cancer cells treated with RSV or IGF-1, respectively. Proteomic analysis enabled us to establish PPP and the talin-pFAK as targets of RSV which suppress cancer cell proliferation and induce apoptosis in the colon cancer cell line HT-29. RSV (150 μM) suppressed these pathways in the presence and absence of IGF-1, suggesting its role as a chemo-preventive agent even in obese condition.
         datePublished:2011-08-17T00:00:00Z
         dateModified:2011-08-17T00:00:00Z
         pageStart:1
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            Proteomics
            Talin
            Focal Adhesion Kinase (FAK)
            Pentose Phosphate Pathway
            Insulin-like Growth Factor-1 (IGF-1)
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ScholarlyArticle:
      headline:Resveratrol suppresses human colon cancer cell proliferation and induces apoptosis via targeting the pentose phosphate and the talin-FAK signaling pathways-A proteomic approach
      description:We and others have previously reported that resveratrol (RSV) suppresses colon cancer cell proliferation and elevates apoptosis in vitro and/or in vivo, however molecular mechanisms are not fully elucidated. Particularly, little information is available on RSV's effects on metabolic pathways and the cell-extra cellular matrix (ECM) communication that are critical for cancer cell growth. To identify important targets of RSV, we analyzed whole protein fractions from HT-29 advanced human colon cancer cell line treated with solvent control, IGF-1 (10 nM) and RSV (150 μM) using LC/MS/MS-Mud PIT (Multidimensional Protein Identification Technology). Pentose phosphate pathway (PPP), a vital metabolic pathway for cell cycle progression, was elevated and suppressed by IGF-1 and RSV, respectively in the HT-29 cell line. Enzymatic assays confirmed RSV suppression of glucose-6 phosphate dehydrogenase (rate limiting) and transketolase, key enzymes of the PPP. RSV (150 μM) suppressed, whereas IGF-1 (10 nM) elevated focal adhesion complex (FAC) proteins, talin and pFAK, critical for the cell-ECM communication. Western blotting analyses confirmed the suppression or elevation of these proteins in HT-29 cancer cells treated with RSV or IGF-1, respectively. Proteomic analysis enabled us to establish PPP and the talin-pFAK as targets of RSV which suppress cancer cell proliferation and induce apoptosis in the colon cancer cell line HT-29. RSV (150 μM) suppressed these pathways in the presence and absence of IGF-1, suggesting its role as a chemo-preventive agent even in obese condition.
      datePublished:2011-08-17T00:00:00Z
      dateModified:2011-08-17T00:00:00Z
      pageStart:1
      pageEnd:11
      license:http://creativecommons.org/licenses/by/2.0
      sameAs:https://doi.org/10.1186/1477-5956-9-49
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         Resveratrol
         Proteomics
         Talin
         Focal Adhesion Kinase (FAK)
         Pentose Phosphate Pathway
         Insulin-like Growth Factor-1 (IGF-1)
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            address:
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               type:PostalAddress
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            name:University of Colorado Cancer Center
            address:
               name:Cancer Prevention and Control Program, University of Colorado Cancer Center, Aurora, USA
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      name:Sridhar Radhakrishnan
      affiliation:
            name:Colorado State University
            address:
               name:Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, USA
               type:PostalAddress
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            name:Colorado State University
            address:
               name:Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, USA
               type:PostalAddress
            type:Organization
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      affiliation:
            name:Scott & White Hospital
            address:
               name:Department of Pathology, Scott & White Hospital, Temple, USA
               type:PostalAddress
            type:Organization
            name:Agilent Technologies
            address:
               name:Agilent Technologies, Wilmington, USA
               type:PostalAddress
            type:Organization
      name:Andrey Ptitsyn
      affiliation:
            name:Colorado State University
            address:
               name:Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, USA
               type:PostalAddress
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      name:Cancer Prevention and Control Program, University of Colorado Cancer Center, Aurora, USA
      name:Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, USA
      name:Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, USA
      name:Department of Pathology, Scott & White Hospital, Temple, USA
      name:Agilent Technologies, Wilmington, USA
      name:Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, USA

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