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Keywords {🔍}

cells, qds, cell, imaging, labeled, article, vivo, stem, figure, google, scholar, quantum, cas, excitation, labeling, shown, day, embryonic, intensity, dots, differentiation, image, analysis, mouse, qtracker, signal, proliferation, emission, multiplex, time, fluorescent, therapy, light, unlabeled, spectra, study, images, assay, authors, viability, mice, fluorescence, due, detection, efficiency, original, vitro, wavelength, live, animals,

Topics {✒️}

jiang hong rao & joseph embryoid body formation mouse embryonic stem embryoid body zongjin li embryonic stem cells total signal-background/exposure time neural cell based water-soluble quantum dots es cell-derived teratomas quantum-dot-tagged microbeads neural cell formation snm bradley-alavi fellowship embryonic stem article  google scholar author correspondence open access article niche-dependent translineage commitment stem cell therapy article download pdf uvp bio-imaging systems peptide-based reagent qtracker embryonic marker produce embryoid bodies semiconductor quantum dots transplanted stem cells adult stem cells cloning stem cells quantum dot imaging es cell differentiation total signal-background es cell growth expressed specific markers quantum dots encapsulated tracking stem cells quantum dot bioconjugates mouse es cells infrared quantum dots fluorescence microscopy multiphoton fluorescence imaging cell-based therapy squamous cell differentiation fluorescence intensity increased naval research laboratory qd-labeled es cells labeled es cell fluorescence microplate reader rt-pcr analysis showed privacy choices/manage cookies authors’ original file

Questions {❓}

• In particular, what are the fewest number of labeled cells that can be detected by the Maestro system and for what duration?

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WebPage:
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         headline:Quantum dot imaging for embryonic stem cells
         description:Semiconductor quantum dots (QDs) hold increasing potential for cellular imaging both in vitro and in vivo. In this report, we aimed to evaluate in vivo multiplex imaging of mouse embryonic stem (ES) cells labeled with Qtracker delivered quantum dots (QDs). Murine embryonic stem (ES) cells were labeled with six different QDs using Qtracker. ES cell viability, proliferation, and differentiation were not adversely affected by QDs compared with non-labeled control cells (P = NS). Afterward, labeled ES cells were injected subcutaneously onto the backs of athymic nude mice. These labeled ES cells could be imaged with good contrast with one single excitation wavelength. With the same excitation wavelength, the signal intensity, defined as (total signal-background)/exposure time in millisecond was 11 ± 2 for cells labeled with QD 525, 12 ± 9 for QD 565, 176 ± 81 for QD 605, 176 ± 136 for QD 655, 167 ± 104 for QD 705, and 1,713 ± 482 for QD 800. Finally, we have shown that QD 800 offers greater fluorescent intensity than the other QDs tested. In summary, this is the first demonstration of in vivo multiplex imaging of mouse ES cells labeled QDs. Upon further improvements, QDs will have a greater potential for tracking stem cells within deep tissues. These results provide a promising tool for imaging stem cell therapy non-invasively in vivo.
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            Neural Cell Adhesion Molecule
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            Mouse Embryonic Stem Cell
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            Applied Microbiology
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      headline:Quantum dot imaging for embryonic stem cells
      description:Semiconductor quantum dots (QDs) hold increasing potential for cellular imaging both in vitro and in vivo. In this report, we aimed to evaluate in vivo multiplex imaging of mouse embryonic stem (ES) cells labeled with Qtracker delivered quantum dots (QDs). Murine embryonic stem (ES) cells were labeled with six different QDs using Qtracker. ES cell viability, proliferation, and differentiation were not adversely affected by QDs compared with non-labeled control cells (P = NS). Afterward, labeled ES cells were injected subcutaneously onto the backs of athymic nude mice. These labeled ES cells could be imaged with good contrast with one single excitation wavelength. With the same excitation wavelength, the signal intensity, defined as (total signal-background)/exposure time in millisecond was 11 ± 2 for cells labeled with QD 525, 12 ± 9 for QD 565, 176 ± 81 for QD 605, 176 ± 136 for QD 655, 167 ± 104 for QD 705, and 1,713 ± 482 for QD 800. Finally, we have shown that QD 800 offers greater fluorescent intensity than the other QDs tested. In summary, this is the first demonstration of in vivo multiplex imaging of mouse ES cells labeled QDs. Upon further improvements, QDs will have a greater potential for tracking stem cells within deep tissues. These results provide a promising tool for imaging stem cell therapy non-invasively in vivo.
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      dateModified:2007-10-09T00:00:00Z
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         Embryonic Stem Cell
         Neural Cell Adhesion Molecule
         Embryoid Body
         Mouse Embryonic Stem Cell
         Embryonic Stem Cell Differentiation
         Applied Microbiology
         Biotechnology
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         Genetic Engineering
         Plant Breeding/Biotechnology
         Biomedical Engineering/Biotechnology
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      name:Molecular Imaging Program at Stanford (MIPS) and Bio-X Program, Department of Radiology, Stanford University, Stanford, USA
      name:Department of Bioengineering, Stanford University, Stanford, USA

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