We are analyzing https://link.springer.com/article/10.1186/1471-2407-9-152.

Title:
High-resolution DNA copy number and gene expression analyses distinguish chromophobe renal cell carcinomas and renal oncocytomas | BMC Cancer
Description:
Background The diagnosis of benign renal oncocytomas (RO) and chromophobe renal cell carcinomas (RCC) based on their morphology remains uncertain in several cases. Methods We have applied Affymetrix GeneChip Mapping 250 K NspI high-density oligoarrays to identify small genomic alterations, which may occur beyond the specific losses of entire chromosomes, and also Affymetrix GeneChip HG-U133 Plus2.0 oligoarrays for gene expression profiling. Results By analysing of DNA extracted from 30 chRCCs and 42 ROs, we have confirmed the high specificity of monosomies of chromosomes 1, 2, 6, 10, 13, 17 and 21 in 70–93% of the chRCCs, while ROs displayed loss of chromosome 1 and 14 in 24% and 5% of the cases, respectively. We demonstrated that chromosomal gene expression biases might correlate with chromosomal abnormalities found in chromophobe RCCs and ROs. The vast majority genes downregulated in chromophobe RCC were mapped to chromosomes 2, 6, 10, 13 and 17. However, most of the genes overexpressed in chromophobe RCCs were located to chromosomes without any copy number changes indicating a transcriptional regulation as a main event. Conclusion The SNP-array analysis failed to detect recurrent small deletions, which may mark loci of genes involved in the tumor development. However, we have identified loss of chromosome 2, 10, 13, 17 and 21 as discriminating alteration between chromophobe RCCs and ROs. Therefore, detection of these chromosomal changes can be used for the accurate diagnosis in routine histology.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Health & Fitness

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,603,474 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't see how the site brings in money.

While profit motivates many websites, others exist to inspire, entertain, or provide valuable resources. Websites have a variety of goals. And this might be one of them. Link.springer.com could be secretly minting cash, but we can't detect the process.

Keywords {🔍}

chromophobe, renal, expression, rccs, chromosome, loss, chromosomes, gene, cell, ros, genes, article, google, scholar, number, pubmed, copy, cas, tumor, chromosomal, dna, analysis, kovacs, figure, cancer, tumors, specific, carcinoma, rcc, cases, data, losses, found, carcinomas, oncocytomas, genetic, pathol, alterations, authors, small, genomic, entire, pcr, diagnosis, affymetrix, genechip, chrccs, molecular, oncocytoma, types,

Topics {✒️}

clear cell sarcoma applied high-density snp-oligoarray high-density snp-based oligoarrays open access article pre-publication history obtain genome-wide view color-coded hmm model nspi high-density oligoarrays quantative real-time pcr quantitative real-time pcr renal cell carcinoma snp-array analysis failed expression profile birt-hogg-dube syndrome renal cell carcinomas single genomic change börje ljungberg additional large-scale studies web-interface gwa http quantitative rt-pcr analysis renal cell cancer author information authors related subjects article download pdf high-quality total rna gene expression profiles renal cell tumours including background adjustment hg-u133 plus2 rijn van de predicted cytogenetic profiles renal cell tumors random genetic change representative genome view relative expression profiles berg van den kidney-specific cadherin dna copy number privacy choices/manage cookies cell junction proteins collecting duct carcinomas spindle cell tumor ruprecht-karls-university heidelberg bmc cancer 9 copy number alterations chromosomal profiles correspond wilhelm sander stiftung copy number intensities copy number analyser renal oncocytomas irrespectively

Schema {🗺️}

WebPage:
      mainEntity:
         headline:High-resolution DNA copy number and gene expression analyses distinguish chromophobe renal cell carcinomas and renal oncocytomas
         description:The diagnosis of benign renal oncocytomas (RO) and chromophobe renal cell carcinomas (RCC) based on their morphology remains uncertain in several cases. We have applied Affymetrix GeneChip Mapping 250 K NspI high-density oligoarrays to identify small genomic alterations, which may occur beyond the specific losses of entire chromosomes, and also Affymetrix GeneChip HG-U133 Plus2.0 oligoarrays for gene expression profiling. By analysing of DNA extracted from 30 chRCCs and 42 ROs, we have confirmed the high specificity of monosomies of chromosomes 1, 2, 6, 10, 13, 17 and 21 in 70–93% of the chRCCs, while ROs displayed loss of chromosome 1 and 14 in 24% and 5% of the cases, respectively. We demonstrated that chromosomal gene expression biases might correlate with chromosomal abnormalities found in chromophobe RCCs and ROs. The vast majority genes downregulated in chromophobe RCC were mapped to chromosomes 2, 6, 10, 13 and 17. However, most of the genes overexpressed in chromophobe RCCs were located to chromosomes without any copy number changes indicating a transcriptional regulation as a main event. The SNP-array analysis failed to detect recurrent small deletions, which may mark loci of genes involved in the tumor development. However, we have identified loss of chromosome 2, 10, 13, 17 and 21 as discriminating alteration between chromophobe RCCs and ROs. Therefore, detection of these chromosomal changes can be used for the accurate diagnosis in routine histology.
         datePublished:2009-05-18T00:00:00Z
         dateModified:2009-05-18T00:00:00Z
         pageStart:1
         pageEnd:10
         license:https://creativecommons.org/licenses/by/2.0
         sameAs:https://doi.org/10.1186/1471-2407-9-152
         keywords:
            Copy Number Change
            Chromosomal Loss
            Entire Chromosome
            Renal Cell Carcinoma
            Clear Cell Sarcoma
            Cancer Research
            Oncology
            Surgical Oncology
            Health Promotion and Disease Prevention
            Biomedicine
            general
            Medicine/Public Health
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig1_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig2_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig3_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig4_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig5_HTML.jpg
         isPartOf:
            name:BMC Cancer
            issn:
               1471-2407
            volumeNumber:9
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Maria V Yusenko
               affiliation:
                     name:Ruprecht-Karls-University
                     address:
                        name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Roland P Kuiper
               affiliation:
                     name:Radboud University Nijmegen Medical Centre
                     address:
                        name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Tamas Boethe
               affiliation:
                     name:University of Pécs Medical School
                     address:
                        name:Department of Urology, University of Pécs Medical School, Pécs, Hungary
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Börje Ljungberg
               affiliation:
                     name:Umeå University
                     address:
                        name:Department of Urology, Umeå University, Umeå, Sweden
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ad Geurts van Kessel
               affiliation:
                     name:Radboud University Nijmegen Medical Centre
                     address:
                        name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Gyula Kovacs
               affiliation:
                     name:Ruprecht-Karls-University
                     address:
                        name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:High-resolution DNA copy number and gene expression analyses distinguish chromophobe renal cell carcinomas and renal oncocytomas
      description:The diagnosis of benign renal oncocytomas (RO) and chromophobe renal cell carcinomas (RCC) based on their morphology remains uncertain in several cases. We have applied Affymetrix GeneChip Mapping 250 K NspI high-density oligoarrays to identify small genomic alterations, which may occur beyond the specific losses of entire chromosomes, and also Affymetrix GeneChip HG-U133 Plus2.0 oligoarrays for gene expression profiling. By analysing of DNA extracted from 30 chRCCs and 42 ROs, we have confirmed the high specificity of monosomies of chromosomes 1, 2, 6, 10, 13, 17 and 21 in 70–93% of the chRCCs, while ROs displayed loss of chromosome 1 and 14 in 24% and 5% of the cases, respectively. We demonstrated that chromosomal gene expression biases might correlate with chromosomal abnormalities found in chromophobe RCCs and ROs. The vast majority genes downregulated in chromophobe RCC were mapped to chromosomes 2, 6, 10, 13 and 17. However, most of the genes overexpressed in chromophobe RCCs were located to chromosomes without any copy number changes indicating a transcriptional regulation as a main event. The SNP-array analysis failed to detect recurrent small deletions, which may mark loci of genes involved in the tumor development. However, we have identified loss of chromosome 2, 10, 13, 17 and 21 as discriminating alteration between chromophobe RCCs and ROs. Therefore, detection of these chromosomal changes can be used for the accurate diagnosis in routine histology.
      datePublished:2009-05-18T00:00:00Z
      dateModified:2009-05-18T00:00:00Z
      pageStart:1
      pageEnd:10
      license:https://creativecommons.org/licenses/by/2.0
      sameAs:https://doi.org/10.1186/1471-2407-9-152
      keywords:
         Copy Number Change
         Chromosomal Loss
         Entire Chromosome
         Renal Cell Carcinoma
         Clear Cell Sarcoma
         Cancer Research
         Oncology
         Surgical Oncology
         Health Promotion and Disease Prevention
         Biomedicine
         general
         Medicine/Public Health
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig1_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig2_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig3_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig4_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1471-2407-9-152/MediaObjects/12885_2009_Article_1486_Fig5_HTML.jpg
      isPartOf:
         name:BMC Cancer
         issn:
            1471-2407
         volumeNumber:9
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Maria V Yusenko
            affiliation:
                  name:Ruprecht-Karls-University
                  address:
                     name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Roland P Kuiper
            affiliation:
                  name:Radboud University Nijmegen Medical Centre
                  address:
                     name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tamas Boethe
            affiliation:
                  name:University of Pécs Medical School
                  address:
                     name:Department of Urology, University of Pécs Medical School, Pécs, Hungary
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Börje Ljungberg
            affiliation:
                  name:Umeå University
                  address:
                     name:Department of Urology, Umeå University, Umeå, Sweden
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ad Geurts van Kessel
            affiliation:
                  name:Radboud University Nijmegen Medical Centre
                  address:
                     name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gyula Kovacs
            affiliation:
                  name:Ruprecht-Karls-University
                  address:
                     name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:BMC Cancer
      issn:
         1471-2407
      volumeNumber:9
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Ruprecht-Karls-University
      address:
         name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany
         type:PostalAddress
      name:Radboud University Nijmegen Medical Centre
      address:
         name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
         type:PostalAddress
      name:University of Pécs Medical School
      address:
         name:Department of Urology, University of Pécs Medical School, Pécs, Hungary
         type:PostalAddress
      name:Umeå University
      address:
         name:Department of Urology, Umeå University, Umeå, Sweden
         type:PostalAddress
      name:Radboud University Nijmegen Medical Centre
      address:
         name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
         type:PostalAddress
      name:Ruprecht-Karls-University
      address:
         name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Maria V Yusenko
      affiliation:
            name:Ruprecht-Karls-University
            address:
               name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany
               type:PostalAddress
            type:Organization
      name:Roland P Kuiper
      affiliation:
            name:Radboud University Nijmegen Medical Centre
            address:
               name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
               type:PostalAddress
            type:Organization
      name:Tamas Boethe
      affiliation:
            name:University of Pécs Medical School
            address:
               name:Department of Urology, University of Pécs Medical School, Pécs, Hungary
               type:PostalAddress
            type:Organization
      name:Börje Ljungberg
      affiliation:
            name:Umeå University
            address:
               name:Department of Urology, Umeå University, Umeå, Sweden
               type:PostalAddress
            type:Organization
      name:Ad Geurts van Kessel
      affiliation:
            name:Radboud University Nijmegen Medical Centre
            address:
               name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
               type:PostalAddress
            type:Organization
      name:Gyula Kovacs
      affiliation:
            name:Ruprecht-Karls-University
            address:
               name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany
      name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
      name:Department of Urology, University of Pécs Medical School, Pécs, Hungary
      name:Department of Urology, Umeå University, Umeå, Sweden
      name:Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
      name:Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany

External Links {🔗}(148)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

5.24s.

LINK . SPRINGER . COM {}