We are analyzing https://link.springer.com/article/10.1186/1471-2334-11-78.

Title:
Association of mutation patterns in gyrA/B genes and ofloxacin resistance levels in Mycobacterium tuberculosisisolates from East China in 2009 | BMC Infectious Diseases
Description:
Background This study aimed to analyze the association of mutation patterns in gyrA and gyrB genes and the ofloxacin resistance levels in clinical Mycobacterium tuberculosis isolates sampled in 2009 from East China. Methods The quinolone resistance-determining region of gyrA/B were sequenced in 192 M. tuberculosis clinical isolates and the minimal inhibitory concentrations (MICs) of 95 ofloxacin-resistant M. tuberculosis isolates were determined by using microplate nitrate reductase assays. Results Mutations in gyrA (codons 90, 91 and 94) and in gyrB (G551R, D500N, T539N, R485C/L) were observed in 89.5% (85/95) and 11.6% (11/95) of ofloxacin-resistant strains, respectively. The gyrB mutations G551R and G549D were observed in 4.1% (4/97) of ofloxacin-susceptible strains and no mutation was found in gyrA in ofloxacin-susceptible strains. The MICs of all ofloxacin-resistant strains showed no significant difference among strains with mutations at codons 90, 91 or 94 in gyrA (F = 1.268, p = 0.287). No differences were detected among strains with different amino acid mutations in the quinolone resistance-determining region of gyrA (F = 1.877, p = 0.123). The difference in MICs between ofloxacin-resistant strains with mutations in gyrA only and ofloxacin-resistant strains with mutations in both gyrA and gyrB genes was not statistically significant (F = 0.549, p = 0.461). Conclusions Although gyrA/B mutations can lead to ofloxacin resistance in M. tuberculosis, there were no associations of different mutation patterns in gyrA/B and the level of ofloxacin resistance in M. tuberculosis isolates from East China in 2009.
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Keywords {🔍}

mutations, strains, mtb, resistance, gyra, tuberculosis, pubmed, article, gyrb, ofxresistant, isolates, mycobacterium, google, scholar, mutation, ofx, study, mics, cas, central, codon, analysis, codons, patterns, genes, clinical, acid, drug, gene, dna, amino, antimicrob, china, agents, mgl, chemother, showed, ofxsusceptible, levels, quinolone, susceptibility, strain, shanghai, data, access, wang, level, gyragyrb, microbiol, gyrab,

Topics {✒️}

pre-publication history xiaochen huang & zhongyi hu extensively drug-resistant tuberculosis multidrug-resistant tuberculosis patients extensively drug-resistant tb anti-tubercular therapeutic agents anti-tuberculosis drug resistance mtb drug-resistant isolates shanghai rising-star program article download pdf fluoroquinolone-resistant mycobacterium tuberculosis fq-resistant mtb strains quinolone resistance-determining region fq-resistant mtb isolates ofx-resistant mtb strains ofx-susceptible strains displayed fq-susceptible mtb isolates ofx-resistant mtb strain ofx-susceptible mtb strains mdr-tb isolates resistant ofloxacin-resistant strains showed zhongyi hu revised multidrug-resistant tuberculosis infectious disease control low-level fq resistance xiaochen huang performed drug resistant tuberculosis high-level fluoroquinolone resistance full access privacy choices/manage cookies da silva pa amino acid substitution shanghai key laboratory mycobacterium tuberculosis complex mycobacterium tuberculosis isolates multicenter laboratory validation ofloxacin-resistant strains 95 ofx-resistant strains ofx-resistant strains line tuberculosis drug mycobacterium tuberculosis highlights ofx-resistant isolates tuberculosis clinical isolates amino acid substitutions biomed central fq-resistant tb single silent mutation mycobacterium tuberculosis resistance antimicrobial exposure amino acid mutations

Questions {❓}

Poissy J, Aubry A, Fernandez C, Lott MC, Chauffour A, Jarlier V, Farinotti R, Veziris N: Should moxifloxacin be used for the treatment of extensively drug-resistant tuberculosis?

Schema {🗺️}

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         headline:Association of mutation patterns in gyrA/B genes and ofloxacin resistance levels in Mycobacterium tuberculosisisolates from East China in 2009
         description:This study aimed to analyze the association of mutation patterns in gyrA and gyrB genes and the ofloxacin resistance levels in clinical Mycobacterium tuberculosis isolates sampled in 2009 from East China. The quinolone resistance-determining region of gyrA/B were sequenced in 192 M. tuberculosis clinical isolates and the minimal inhibitory concentrations (MICs) of 95 ofloxacin-resistant M. tuberculosis isolates were determined by using microplate nitrate reductase assays. Mutations in gyrA (codons 90, 91 and 94) and in gyrB (G551R, D500N, T539N, R485C/L) were observed in 89.5% (85/95) and 11.6% (11/95) of ofloxacin-resistant strains, respectively. The gyrB mutations G551R and G549D were observed in 4.1% (4/97) of ofloxacin-susceptible strains and no mutation was found in gyrA in ofloxacin-susceptible strains. The MICs of all ofloxacin-resistant strains showed no significant difference among strains with mutations at codons 90, 91 or 94 in gyrA (F = 1.268, p = 0.287). No differences were detected among strains with different amino acid mutations in the quinolone resistance-determining region of gyrA (F = 1.877, p = 0.123). The difference in MICs between ofloxacin-resistant strains with mutations in gyrA only and ofloxacin-resistant strains with mutations in both gyrA and gyrB genes was not statistically significant (F = 0.549, p = 0.461). Although gyrA/B mutations can lead to ofloxacin resistance in M. tuberculosis, there were no associations of different mutation patterns in gyrA/B and the level of ofloxacin resistance in M. tuberculosis isolates from East China in 2009.
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      description:This study aimed to analyze the association of mutation patterns in gyrA and gyrB genes and the ofloxacin resistance levels in clinical Mycobacterium tuberculosis isolates sampled in 2009 from East China. The quinolone resistance-determining region of gyrA/B were sequenced in 192 M. tuberculosis clinical isolates and the minimal inhibitory concentrations (MICs) of 95 ofloxacin-resistant M. tuberculosis isolates were determined by using microplate nitrate reductase assays. Mutations in gyrA (codons 90, 91 and 94) and in gyrB (G551R, D500N, T539N, R485C/L) were observed in 89.5% (85/95) and 11.6% (11/95) of ofloxacin-resistant strains, respectively. The gyrB mutations G551R and G549D were observed in 4.1% (4/97) of ofloxacin-susceptible strains and no mutation was found in gyrA in ofloxacin-susceptible strains. The MICs of all ofloxacin-resistant strains showed no significant difference among strains with mutations at codons 90, 91 or 94 in gyrA (F = 1.268, p = 0.287). No differences were detected among strains with different amino acid mutations in the quinolone resistance-determining region of gyrA (F = 1.877, p = 0.123). The difference in MICs between ofloxacin-resistant strains with mutations in gyrA only and ofloxacin-resistant strains with mutations in both gyrA and gyrB genes was not statistically significant (F = 0.549, p = 0.461). Although gyrA/B mutations can lead to ofloxacin resistance in M. tuberculosis, there were no associations of different mutation patterns in gyrA/B and the level of ofloxacin resistance in M. tuberculosis isolates from East China in 2009.
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