We are analyzing https://link.springer.com/article/10.1186/1471-230x-10-134.

Title:
Ulcerative colitis and irritable bowel patients exhibit distinct abnormalities of the gut microbiota | BMC Gastroenterology
Description:
Background Previous studies suggest a link between gut microbiota and the development of ulcerative colitis (UC) and irritable bowel syndrome (IBS). Our aim was to investigate any quantitative differences in faecal bacterial compositions in UC and IBS patients compared to healthy controls, and to identify individual bacterial species that contribute to these differences. Methods Faecal microbiota of 13 UC patients, 11 IBS patients and 22 healthy volunteers were analysed by PCR-Denaturing Gradient Gel Electrophoresis (DGGE) using universal and Bacteroides specific primers. The data obtained were normalized using in-house developed statistical method and interrogated by multivariate approaches. The differentiated bands were excised and identified by sequencing the V3 region of the 16S rRNA genes. Results Band profiles revealed that number of predominant faecal bacteria were significantly different between UC, IBS and control group (p < 10-4). By assessing the mean band numbers in UC (37 ± 5) and IBS (39 ± 6), compared to the controls (45 ± 3), a significant decrease in bacterial species is suggested (p = 0.01). There were no significant differences between IBS and UC. Biodiversity of the bacterial species was significantly lower in UC (μ = 2.94, σ = 0.29) and IBS patients (μ = 2.90, σ = 0.38) than controls (μ = 3.25, σ = 0.16; p = 0.01). Moreover, similarity indices revealed greater biological variability of predominant bacteria in UC and IBS compared to the controls (median Dice coefficients 76.1% (IQR 70.9 - 83.1), 73.8% (IQR 67.0 - 77.5) and 82.9% (IQR 79.1 - 86.7) respectively). DNA sequencing of discriminating bands suggest that the presence of Bacteroides vulgatus, B. ovatus, B. uniformis, and Parabacteroides sp. in healthy volunteers distinguishes them from IBS and UC patients. DGGE profiles of Bacteroides species revealed a decrease of Bacteroides community in UC relative to IBS and controls. Conclusion Molecular profiling of faecal bacteria revealed abnormalities of intestinal microbiota in UC and IBS patients, while different patterns of Bacteroides species loss in particular, were associated with UC and IBS.
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ibs, patients, article, pubmed, google, scholar, bacteria, cas, bacterial, bowel, dgge, microbiota, controls, data, analysis, bacteroides, gut, bands, gel, faecal, disease, colitis, figure, band, gastroenterology, healthy, microbial, study, journal, irritable, species, predominant, inflammatory, number, syndrome, intestinal, human, samples, groups, profiles, reported, central, ulcerative, studies, differences, authors, alignment, diversity, research, full,

Topics {✒️}

tcr alpha-chain-deficient mice germ-free hla-b27 rats open access article hla-b27 transgenic rats escherichia coli-induced colitis top-ranked discriminating bands hla-b27/human beta gnotobiotic interleukin-2-deficient mice article download pdf il-10 gene-deficient mice pair-wise similarity coefficients irritable bowel syndrome pre-publication history active ulcerative colitis pcr-amplified 16s rdna mediate chronic colitis pls-da permutation tests dextran-sodium sulphate canonical variate analysis full size image culture-independent profiling methods semi-quantitative dgge analysis diverse bacterial community microglobulin transgenic rats top-ranked features bacterial bowel communities complex microbial communities mixed microbial communities privacy choices/manage cookies squares discriminant analysis human lower intestinal-tract fecal microbial-population germ-free environment [8] normal commensal bacteria top-ranked bands authors’ original file 16s rrna genes 16s rrna gene bifidobacterium longum/synergy 1 ulcerative colitis human molecular genetics inflammatory bowel diseases fecal bacterial populations band-count findings reported full access discriminating bands suggest bacterial populations differed protect germ-free abnormal colonic fermentation inflammatory bowel disease

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Sokol H, Lay C, Seksik P, Tannock GW: Analysis of bacterial bowel communities of IBD patients: What has it revealed?

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         headline:Ulcerative colitis and irritable bowel patients exhibit distinct abnormalities of the gut microbiota
         description:Previous studies suggest a link between gut microbiota and the development of ulcerative colitis (UC) and irritable bowel syndrome (IBS). Our aim was to investigate any quantitative differences in faecal bacterial compositions in UC and IBS patients compared to healthy controls, and to identify individual bacterial species that contribute to these differences. Faecal microbiota of 13 UC patients, 11 IBS patients and 22 healthy volunteers were analysed by PCR-Denaturing Gradient Gel Electrophoresis (DGGE) using universal and Bacteroides specific primers. The data obtained were normalized using in-house developed statistical method and interrogated by multivariate approaches. The differentiated bands were excised and identified by sequencing the V3 region of the 16S rRNA genes. Band profiles revealed that number of predominant faecal bacteria were significantly different between UC, IBS and control group (p < 10-4). By assessing the mean band numbers in UC (37 ± 5) and IBS (39 ± 6), compared to the controls (45 ± 3), a significant decrease in bacterial species is suggested (p = 0.01). There were no significant differences between IBS and UC. Biodiversity of the bacterial species was significantly lower in UC (μ = 2.94, σ = 0.29) and IBS patients (μ = 2.90, σ = 0.38) than controls (μ = 3.25, σ = 0.16; p = 0.01). Moreover, similarity indices revealed greater biological variability of predominant bacteria in UC and IBS compared to the controls (median Dice coefficients 76.1% (IQR 70.9 - 83.1), 73.8% (IQR 67.0 - 77.5) and 82.9% (IQR 79.1 - 86.7) respectively). DNA sequencing of discriminating bands suggest that the presence of Bacteroides vulgatus, B. ovatus, B. uniformis, and Parabacteroides sp. in healthy volunteers distinguishes them from IBS and UC patients. DGGE profiles of Bacteroides species revealed a decrease of Bacteroides community in UC relative to IBS and controls. Molecular profiling of faecal bacteria revealed abnormalities of intestinal microbiota in UC and IBS patients, while different patterns of Bacteroides species loss in particular, were associated with UC and IBS.
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            Irritable Bowel Syndrome Patient
            Partial Little Square Discriminant Analysis
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            Internal Medicine
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         Irritable Bowel Syndrome
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         Irritable Bowel Syndrome Patient
         Partial Little Square Discriminant Analysis
         Gastroenterology
         Internal Medicine
         Hepatology
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