We are analyzing https://link.springer.com/article/10.1186/1471-2229-8-121.

Title:
The MYST histone acetyltransferases are essential for gametophyte development in Arabidopsis | BMC Plant Biology
Description:
Background Histone acetyltransferases (HATs) play critical roles in the regulation of chromatin structure and gene expression. Arabidopsis genome contains 12 HAT genes, but the biological functions of many of them are still unknown. In this work, we studied the evolutionary relationship and cellular functions of the two Arabidopsis HAT genes homologous to the MYST family members. Results An extensive phylogenetic analysis of 105 MYST proteins revealed that they can be divided into 5 classes, each of which contains a specific combination of protein modules. The two Arabidopsis MYST proteins, HAM1 and HAM2, belong to a "green clade", clearly separated from other families of HATs. Using a reverse genetic approach, we show that HAM1 and HAM2 are a functionally redundant pair of genes, as single Arabidopsis ham1 and ham2 mutants displayed a wild-type phenotype, while no double mutant seedling could be recovered. Genetic analysis and cytological study revealed that ham1ham2 double mutation induced severe defects in the formation of male and female gametophyte, resulting in an arrest of mitotic cell cycle at early stages of gametogenesis. RT-PCR experiments and the analysis of transgenic plants expressing the GUS reporter gene under the HAM1 or the HAM2 promoter showed that both genes displayed an overlapping expression pattern, mainly in growing organs such as shoots and flower buds. Conclusion The work presented here reveals novel properties for MYST HATs in Arabidopsis. In addition to providing an evolutionary relationship of this large protein family, we show the evidence of a link between MYST and gamete formation as previously suggested in mammalian cells. A possible function of the Arabidopsis MYST protein-mediated histone acetylation during cell division is suggested.
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Keywords {🔍}

ham, myst, hamham, pubmed, article, arabidopsis, google, scholar, cell, cas, histone, proteins, plant, analysis, wildtype, plants, development, expression, sequences, pollen, mutants, genes, fig, family, mutant, gene, central, file, protein, phylogenetic, gametophyte, chromatin, gus, acetyltransferase, additional, domain, biol, data, double, female, cycle, dna, grains, essential, male, activity, lines, tdna, stage, acetylation,

Topics {✒️}

5-bromo-4-chloro-3-indolyl-_-d-glucoronic acid dao-xiu zhou & marianne delarue article download pdf central moz-sas domain université paris-sud xi t-dna left border ring-finger e3 ligases meiosis-accumulated ick4/krp6 embryonic cell-fate patterning open access article gcn5-related n-acetyltransferase t-dna insertion mutations improm-ii reverse transcriptase t-dna insertion sites ring-type e3 ligases author information authors inducible mis-expression system t-dna insertion lines regulate gene expression central cell nucleus redundantly required post-meiotically primer pairs p1–p2 primer pairs p1-p3 extensive blast search full size image cell cycle progression moz-sas acetyltransferase domain ham t-dna alleles cold-regulated gene expression resulting double-heterozygous f1 cell cycle dependent site-specific histone acetylation wild-type arabidopsis thaliana male/female gametophyte formation view female gametophyte death light-responsive gene expression t-dna insertions wild-type female parent double mutant plant bmc dev biol privacy choices/manage cookies arabidopsis genomic dna ham1ham2 double mutation genomic dna extraction myst-type proteins revealed long-day conditions female gametophyte due mitotic cell cycle t-dna insert

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         description:Histone acetyltransferases (HATs) play critical roles in the regulation of chromatin structure and gene expression. Arabidopsis genome contains 12 HAT genes, but the biological functions of many of them are still unknown. In this work, we studied the evolutionary relationship and cellular functions of the two Arabidopsis HAT genes homologous to the MYST family members. An extensive phylogenetic analysis of 105 MYST proteins revealed that they can be divided into 5 classes, each of which contains a specific combination of protein modules. The two Arabidopsis MYST proteins, HAM1 and HAM2, belong to a "green clade", clearly separated from other families of HATs. Using a reverse genetic approach, we show that HAM1 and HAM2 are a functionally redundant pair of genes, as single Arabidopsis ham1 and ham2 mutants displayed a wild-type phenotype, while no double mutant seedling could be recovered. Genetic analysis and cytological study revealed that ham1ham2 double mutation induced severe defects in the formation of male and female gametophyte, resulting in an arrest of mitotic cell cycle at early stages of gametogenesis. RT-PCR experiments and the analysis of transgenic plants expressing the GUS reporter gene under the HAM1 or the HAM2 promoter showed that both genes displayed an overlapping expression pattern, mainly in growing organs such as shoots and flower buds. The work presented here reveals novel properties for MYST HATs in Arabidopsis. In addition to providing an evolutionary relationship of this large protein family, we show the evidence of a link between MYST and gamete formation as previously suggested in mammalian cells. A possible function of the Arabidopsis MYST protein-mediated histone acetylation during cell division is suggested.
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            Abort Ovule
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         Agriculture
         Tree Biology
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      name:Institut de Biotechnologie des Plantes, CNRS UMR8618, Université Paris-sud XI, Orsay, France
      name:Institut de Biotechnologie des Plantes, CNRS UMR8618, Université Paris-sud XI, Orsay, France
      name:Institut de Biotechnologie des Plantes, CNRS UMR8618, Université Paris-sud XI, Orsay, France
      name:Institut de Biotechnologie des Plantes, CNRS UMR8618, Université Paris-sud XI, Orsay, France
      name:Institut de Biotechnologie des Plantes, CNRS UMR8618, Université Paris-sud XI, Orsay, France

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