We are analyzing https://link.springer.com/article/10.1186/1471-2180-9-249.

Title:
Recombinant porcine rotavirus VP4 and VP4-LTB expressed in Lactobacillus casei induced mucosal and systemic antibody responses in mice | BMC Microbiology
Description:
Background Porcine rotavirus infection is a significant cause of morbidity and mortality in the swine industry necessitating the development of effective vaccines for the prevention of infection. Immune responses associated with protection are primarily mucosal in nature and induction of mucosal immunity is important for preventing porcine rotavirus infection. Results Lactobacillus casei expressing the major protective antigen VP4 of porcine rotavirus (pPG612.1-VP4) or VP4-LTB (heat-labile toxin B subunit from Echerichia coli) (pPG612.1-VP4-LTB) fusion protein was used to immunize mice orally. The expression of recombinant pPG612.1-VP4 and pPG612.1-VP4-LTB was confirmed by SDS-PAGE and Western blot analysis and surface-displayed expression on L. casei was verified by immunofluorescence. Mice orally immunized with recombinant protein-expressing L. casei produced high levels of serum immunoglobulin G (IgG) and mucosal IgA. The IgA titters from mice immunized with pPG612.1-VP4-LTB were higher than titters from pPG612.1-VP4-immunized mice. The induced antibodies demonstrated neutralizing effects on RV infection. Conclusion These results demonstrated that VP4 administered in the context of an L. casei expression system is an effective method for stimulating mucosal immunity and that LTB served to further stimulate mucosal immunity suggesting that this strategy can be adapted for use in pigs.
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Topics {✒️}

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WebPage:
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         headline:Recombinant porcine rotavirus VP4 and VP4-LTB expressed in Lactobacillus casei induced mucosal and systemic antibody responses in mice
         description:Porcine rotavirus infection is a significant cause of morbidity and mortality in the swine industry necessitating the development of effective vaccines for the prevention of infection. Immune responses associated with protection are primarily mucosal in nature and induction of mucosal immunity is important for preventing porcine rotavirus infection. Lactobacillus casei expressing the major protective antigen VP4 of porcine rotavirus (pPG612.1-VP4) or VP4-LTB (heat-labile toxin B subunit from Echerichia coli) (pPG612.1-VP4-LTB) fusion protein was used to immunize mice orally. The expression of recombinant pPG612.1-VP4 and pPG612.1-VP4-LTB was confirmed by SDS-PAGE and Western blot analysis and surface-displayed expression on L. casei was verified by immunofluorescence. Mice orally immunized with recombinant protein-expressing L. casei produced high levels of serum immunoglobulin G (IgG) and mucosal IgA. The IgA titters from mice immunized with pPG612.1-VP4-LTB were higher than titters from pPG612.1-VP4-immunized mice. The induced antibodies demonstrated neutralizing effects on RV infection. These results demonstrated that VP4 administered in the context of an L. casei expression system is an effective method for stimulating mucosal immunity and that LTB served to further stimulate mucosal immunity suggesting that this strategy can be adapted for use in pigs.
         datePublished:2009-12-04T00:00:00Z
         dateModified:2009-12-04T00:00:00Z
         pageStart:1
         pageEnd:11
         license:https://creativecommons.org/licenses/by/2.0
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         keywords:
            Xylose
            Lactic Acid Bacterium
            Recombinant Strain
            MA104 Cell
            Neutralization Ability
            Microbiology
            Biological Microscopy
            Mycology
            Parasitology
            Virology
            Life Sciences
            general
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                        type:PostalAddress
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               type:Person
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               affiliation:
                     name:Northeast Agricultural University
                     address:
                        name:College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yijing Li
               affiliation:
                     name:Northeast Agricultural University
                     address:
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ScholarlyArticle:
      headline:Recombinant porcine rotavirus VP4 and VP4-LTB expressed in Lactobacillus casei induced mucosal and systemic antibody responses in mice
      description:Porcine rotavirus infection is a significant cause of morbidity and mortality in the swine industry necessitating the development of effective vaccines for the prevention of infection. Immune responses associated with protection are primarily mucosal in nature and induction of mucosal immunity is important for preventing porcine rotavirus infection. Lactobacillus casei expressing the major protective antigen VP4 of porcine rotavirus (pPG612.1-VP4) or VP4-LTB (heat-labile toxin B subunit from Echerichia coli) (pPG612.1-VP4-LTB) fusion protein was used to immunize mice orally. The expression of recombinant pPG612.1-VP4 and pPG612.1-VP4-LTB was confirmed by SDS-PAGE and Western blot analysis and surface-displayed expression on L. casei was verified by immunofluorescence. Mice orally immunized with recombinant protein-expressing L. casei produced high levels of serum immunoglobulin G (IgG) and mucosal IgA. The IgA titters from mice immunized with pPG612.1-VP4-LTB were higher than titters from pPG612.1-VP4-immunized mice. The induced antibodies demonstrated neutralizing effects on RV infection. These results demonstrated that VP4 administered in the context of an L. casei expression system is an effective method for stimulating mucosal immunity and that LTB served to further stimulate mucosal immunity suggesting that this strategy can be adapted for use in pigs.
      datePublished:2009-12-04T00:00:00Z
      dateModified:2009-12-04T00:00:00Z
      pageStart:1
      pageEnd:11
      license:https://creativecommons.org/licenses/by/2.0
      sameAs:https://doi.org/10.1186/1471-2180-9-249
      keywords:
         Xylose
         Lactic Acid Bacterium
         Recombinant Strain
         MA104 Cell
         Neutralization Ability
         Microbiology
         Biological Microscopy
         Mycology
         Parasitology
         Virology
         Life Sciences
         general
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                     type:PostalAddress
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            type:Person
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            affiliation:
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                  address:
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                     type:PostalAddress
                  type:Organization
            type:Person
            name:Min Liu
            affiliation:
                  name:Northeast Agricultural University
                  address:
                     name:College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yijing Li
            affiliation:
                  name:Northeast Agricultural University
                  address:
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      address:
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         type:PostalAddress
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      address:
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      affiliation:
            name:Northeast Agricultural University
            address:
               name:Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, Harbin, China
               type:PostalAddress
            type:Organization
      name:Xiaojing Li
      affiliation:
            name:Northeast Agricultural University
            address:
               name:Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, Harbin, China
               type:PostalAddress
            type:Organization
      name:Min Liu
      affiliation:
            name:Northeast Agricultural University
            address:
               name:College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
               type:PostalAddress
            type:Organization
      name:Yijing Li
      affiliation:
            name:Northeast Agricultural University
            address:
               name:Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, Harbin, China
               type:PostalAddress
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      name:Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, Harbin, China
      name:College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
      name:Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, Harbin, China

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