We are analyzing https://link.springer.com/article/10.1186/1471-2164-6-108.

Title:
Comparative analysis of expression of histone H2a genes in mouse | BMC Genomics
Description:
Background At least 18 replication-dependent histone H2a genes are distributed in 3 Hist gene clusters on different chromosomes of the mouse genome. In this analysis we designed specific PCR primers for each histone H2a transcript and studied the expression levels and patterns using quantitative RT-PCR (qRT-PCR). In addition, we compared histone H3 K9 acetylation levels in the promoter regions of H2a genes by ChIP (chromatin immunoprecipitation) – quantitative PCR (qPCR) analysis. Results RT-PCR analysis indicated that all 20 histone H2a genes assessed in this study are expressed. The replication-dependent histone H2a genes have different expression levels but similar expression patterns. Among the 20 histone H2a genes, the expression-level of H2afz, a replication-independent gene, was highest, and that of Hist1h2aa, a replication-dependent gene, was lowest. Among 18 replication-dependent H2a genes, the expression level of Hist3h2a was highest. The ChIP-qPCR analysis showed that histone H3 K9 acetylation levels in promoter regions of both H2afz and Hist3h2a are clearly higher than that in the promoter region of Hist1h2aa. The H3 K9 acetylation level in the promoter of Hist1h2aa is similar to that in the γ-satellite region. Conclusion These results strongly suggest that histone H3 K9 acetylation plays a role in the expression of histone genes.
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Keywords {🔍}

histone, expression, genes, article, pubmed, gene, levels, cell, google, scholar, hafz, analysis, replicationdependent, acetylation, cas, transcript, level, fig, promoter, research, cycle, mouse, pcr, chromatin, histhaa, transcription, mrna, sphase, dna, primers, replicationindependent, central, open, access, nishida, hist, quantitative, histha, proteins, protein, hafx, cells, authors, bmc, full, similar, control, structure, cluster, chromosome,

Topics {✒️}

rna-polymerase-ii-mediated transcription post-transcriptional modifications real-time rt-pcr profiling histone h2a gene yasuhiro tomaru & yoshihide hayashizaki histone gene expression replication-dependent histone genes article download pdf replication-dependent gene transcription-related proteins cooperate replication-independent gene cell-cycle-regulated mrna ending pericentromeric γ-satellite dna eukaryotic chromosomes requires conserved c-terminal sequence open access article takahiro suzuki histone h3 k9 author information authors replication-dependent h2a genes linking site-specific loss real-time quantitative pcr quantitative rt-pcr analysis h3 k9 acetylation chip-qpcr analysis showed human histone h2a histone h2a genes full size image γ-satellite pcr primers histone h2a proteins marzluff wf individual histone genes genomic dna h3 k9 methylation histone mrna accounts histone proteins made histone mrna expression histone h2a transcript h2a gene γ-satellite heterochromatin region privacy choices/manage cookies authors’ original file dna replication type replication-dependent manner gene expression replication-dependent genes 4 replication-dependent genes replication-independent manner genome network project innovational research programs

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Is this modification related to histone gene expression?

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         headline:Comparative analysis of expression of histone H2a genes in mouse
         description:At least 18 replication-dependent histone H2a genes are distributed in 3 Hist gene clusters on different chromosomes of the mouse genome. In this analysis we designed specific PCR primers for each histone H2a transcript and studied the expression levels and patterns using quantitative RT-PCR (qRT-PCR). In addition, we compared histone H3 K9 acetylation levels in the promoter regions of H2a genes by ChIP (chromatin immunoprecipitation) – quantitative PCR (qPCR) analysis. RT-PCR analysis indicated that all 20 histone H2a genes assessed in this study are expressed. The replication-dependent histone H2a genes have different expression levels but similar expression patterns. Among the 20 histone H2a genes, the expression-level of H2afz, a replication-independent gene, was highest, and that of Hist1h2aa, a replication-dependent gene, was lowest. Among 18 replication-dependent H2a genes, the expression level of Hist3h2a was highest. The ChIP-qPCR analysis showed that histone H3 K9 acetylation levels in promoter regions of both H2afz and Hist3h2a are clearly higher than that in the promoter region of Hist1h2aa. The H3 K9 acetylation level in the promoter of Hist1h2aa is similar to that in the γ-satellite region. These results strongly suggest that histone H3 K9 acetylation plays a role in the expression of histone genes.
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            Proteomics
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            Microbial Genetics and Genomics
            Plant Genetics and Genomics
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      headline:Comparative analysis of expression of histone H2a genes in mouse
      description:At least 18 replication-dependent histone H2a genes are distributed in 3 Hist gene clusters on different chromosomes of the mouse genome. In this analysis we designed specific PCR primers for each histone H2a transcript and studied the expression levels and patterns using quantitative RT-PCR (qRT-PCR). In addition, we compared histone H3 K9 acetylation levels in the promoter regions of H2a genes by ChIP (chromatin immunoprecipitation) – quantitative PCR (qPCR) analysis. RT-PCR analysis indicated that all 20 histone H2a genes assessed in this study are expressed. The replication-dependent histone H2a genes have different expression levels but similar expression patterns. Among the 20 histone H2a genes, the expression-level of H2afz, a replication-independent gene, was highest, and that of Hist1h2aa, a replication-dependent gene, was lowest. Among 18 replication-dependent H2a genes, the expression level of Hist3h2a was highest. The ChIP-qPCR analysis showed that histone H3 K9 acetylation levels in promoter regions of both H2afz and Hist3h2a are clearly higher than that in the promoter region of Hist1h2aa. The H3 K9 acetylation level in the promoter of Hist1h2aa is similar to that in the γ-satellite region. These results strongly suggest that histone H3 K9 acetylation plays a role in the expression of histone genes.
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         Histone Gene Expression
         Histone Gene Transcription
         Regulate Histone Gene
         Histone Protein Level
         Life Sciences
         general
         Microarrays
         Proteomics
         Animal Genetics and Genomics
         Microbial Genetics and Genomics
         Plant Genetics and Genomics
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