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LINK . SPRINGER . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. External Links
  11. Analytics And Tracking
  12. Libraries

We are analyzing https://link.springer.com/article/10.1007/s13402-020-00553-1.

Title:
RETRACTED ARTICLE: The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer | Cellular Oncology
Description:
Purpose Growing evidence indicates that aberrant expression of microRNAs contributes to tumor development. However, the biological role of microRNA-4490 (miR-4490) in gastric cancer (GC) remains to be clarified. Methods To explore the function of miR-4490 in GC, we performed colony formation, EdU incorporation, qRT-PCR, Western blotting, in situ hybridization (ISH), immunohistochemistry (IHC), flow cytometry, ChIP and dual-luciferase reporter assays. In addition, the growth, migration and invasion capacities of GC cells were evaluated. Results We found that miR-4490 was significantly downregulated in primary GC samples and in GC-derived cell lines compared with normal controls, and that this expression level was negatively correlated with GC malignancy. Exogenous miR-4490 expression not only reduced cell cycle progression and proliferation, but also significantly inhibited GC cell migration, invasion and epithelial-mesenchymal transition (EMT) in vitro. Mechanistically, we found that miR-4490 directly targets USP22, which mediates inhibition of GC cell proliferation and EMT-induced metastasis in vitro and in vivo. Moreover, we found through luciferase and ChIP assays that transcription factor POU2F1 can directly bind to POU2F1 binding sites within the miR-4490 and USP22 promoters and, by doing so, modulate their transcription. Spearman’s correlation analysis revealed a positive correlation between USP22 and POU2F1 expression and negative correlations between miR-4490 and USP22 as well as miR-4490 and POU2F1 expression in primary GC tissues. Conclusion Based on our results we conclude that miR-4490 acts as a tumor suppressor, and that the POU2F1/miR-4490/USP22 axis plays an important role in the regulation of growth, invasion and EMT of GC cells.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {šŸ“š}

  • Education
  • Science
  • Movies

Content Management System {šŸ“}

What CMS is link.springer.com built with?

Custom-built

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Traffic Estimate {šŸ“ˆ}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
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How Does Link.springer.com Make Money? {šŸ’ø}

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Earning money isn't the goal of every website; some are designed to offer support or promote social causes. People have different reasons for creating websites. This might be one such reason. Link.springer.com has a secret sauce for making money, but we can't detect it yet.

Keywords {šŸ”}

cells, expression, usp, fig, pouf, cell, cancer, article, found, invasion, google, scholar, liu, metastasis, tissues, compared, proliferation, wang, emt, gastric, group, vector, decreased, zhang, promoter, mnc, levels, ecadherin, analysis, data, chen, migration, progression, china, transfected, mice, performed, assays, growth, usa, increased, human, supplementary, yang, tumor, assay, utr, vimentin, luciferase, results,

Topics {āœ’ļø}

activating c-myc/nampt/sirt1-dependent foxo1 ubi-mcs-sv40- egfp-ires es/cgi-bin/promo_v3/promo/promoinit erbb4/pik3r3/mtor/s6k2 signaling pathway alkaline phosphatase-conjugated anti-digoxigenin pou2f1-mir-4490-usp22 signaling axis pou2f1/mir-4490/usp22 signaling axis pou2f1/mir-4490/usp22 axis plays i-nc-transfected cells displayed synbindin-related erk signalling mir-4490/pou2f1-overexpressing group exhibited intimate cell-cell contacts activates wnt/pcp pathway c-jun promotes proliferation semi-quantitative scoring system akt-pou2f1-ecd signalling puromycin-resistant cell pools induces epithelial-mesenchymal transition mir-584-5p inhibits proliferation mouse anti-cyclin b1 dna polymerase-primase complex specific pathogen-free conditions pou2f1/mir-4490/usp22 axis dig-labeled mir-4490 probe gc-derived cell lines quantitative real-time pcr small-cell lung cancer inhibit foxk1-mediated proliferation dual-luciferase reporter assay epithelial marker e-cadherin gc cell cycle progression promotes cell growth pou2f1/mir-4490 group exhibited microrna-193a-3p pou2f1-mediated gc development normal gastric mucosa de/apps/zmf/mirwalk2/ dual-luciferase reporter assays human gastric cancer article download pdf inverted phase-contrast microscope pou2f1-mir-4490 interaction tests pou2f1-expressing cells decreased significant g1/g0 arrest mir-4490-mediated usp22 downregulation pou2f1-overexpressing gc cells human epithelial cancer mir-4490-overexpressing gc cells γ-catenin expression levels vector/mimic-nc group

Questions {ā“}

Schema {šŸ—ŗļø}

WebPage:
      mainEntity:
         headline:RETRACTED ARTICLE: The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer
         description:Growing evidence indicates that aberrant expression of microRNAs contributes to tumor development. However, the biological role of microRNA-4490 (miR-4490) in gastric cancer (GC) remains to be clarified. To explore the function of miR-4490 in GC, we performed colony formation, EdU incorporation, qRT-PCR, Western blotting, in situ hybridization (ISH), immunohistochemistry (IHC), flow cytometry, ChIP and dual-luciferase reporter assays. In addition, the growth, migration and invasion capacities of GC cells were evaluated. We found that miR-4490 was significantly downregulated in primary GC samples and in GC-derived cell lines compared with normal controls, and that this expression level was negatively correlated with GC malignancy. Exogenous miR-4490 expression not only reduced cell cycle progression and proliferation, but also significantly inhibited GC cell migration, invasion and epithelial-mesenchymal transition (EMT) in vitro. Mechanistically, we found that miR-4490 directly targets USP22, which mediates inhibition of GC cell proliferation and EMT-induced metastasis in vitro and in vivo. Moreover, we found through luciferase and ChIP assays that transcription factor POU2F1 can directly bind to POU2F1 binding sites within the miR-4490 and USP22 promoters and, by doing so, modulate their transcription. Spearman’s correlation analysis revealed a positive correlation between USP22 and POU2F1 expression and negative correlations between miR-4490 and USP22 as well as miR-4490 and POU2F1 expression in primary GC tissues. Based on our results we conclude that miR-4490 acts as a tumor suppressor, and that the POU2F1/miR-4490/USP22 axis plays an important role in the regulation of growth, invasion and EMT of GC cells.
         datePublished:2020-08-28T00:00:00Z
         dateModified:2022-12-29T00:00:00Z
         pageStart:1017
         pageEnd:1033
         license:http://creativecommons.org/licenses/by/4.0/
         sameAs:https://doi.org/10.1007/s13402-020-00553-1
         keywords:
            Gastric cancer
            Metastasis
            MiR-4490
            POU2F1
            Epithelial-mesenchymal transition
            Cancer Research
            Biomedicine
            general
            Pathology
            Oncology
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            issn:
               2211-3436
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            volumeNumber:43
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            name:Springer Netherlands
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               name:Yizhi Xiao
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                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
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                     name:Longgang District People’s Hospital
                     address:
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                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jiaying Li
               affiliation:
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                     address:
                        name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
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                        name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
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                     name:Nanfang Hospital, Southern Medical University
                     address:
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               name:Wenjing Zhang
               affiliation:
                     name:the First people’s Hospital of Yunnan Province, Medical School of Kunming University of Science and Technology
                     address:
                        name:Department of Medical Oncology, the First people’s Hospital of Yunnan Province, Medical School of Kunming University of Science and Technology, Kunming, China
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                     address:
                        name:The Second School of Clinical Medicine, Southern Medical University, Guangzhou, People’s Republic of China
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                        name:Department of Gastroenterology, The third affiliated hospital of Guangzhou medical university, Guangzhou, China
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                        name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
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                     address:
                        name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
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ScholarlyArticle:
      headline:RETRACTED ARTICLE: The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer
      description:Growing evidence indicates that aberrant expression of microRNAs contributes to tumor development. However, the biological role of microRNA-4490 (miR-4490) in gastric cancer (GC) remains to be clarified. To explore the function of miR-4490 in GC, we performed colony formation, EdU incorporation, qRT-PCR, Western blotting, in situ hybridization (ISH), immunohistochemistry (IHC), flow cytometry, ChIP and dual-luciferase reporter assays. In addition, the growth, migration and invasion capacities of GC cells were evaluated. We found that miR-4490 was significantly downregulated in primary GC samples and in GC-derived cell lines compared with normal controls, and that this expression level was negatively correlated with GC malignancy. Exogenous miR-4490 expression not only reduced cell cycle progression and proliferation, but also significantly inhibited GC cell migration, invasion and epithelial-mesenchymal transition (EMT) in vitro. Mechanistically, we found that miR-4490 directly targets USP22, which mediates inhibition of GC cell proliferation and EMT-induced metastasis in vitro and in vivo. Moreover, we found through luciferase and ChIP assays that transcription factor POU2F1 can directly bind to POU2F1 binding sites within the miR-4490 and USP22 promoters and, by doing so, modulate their transcription. Spearman’s correlation analysis revealed a positive correlation between USP22 and POU2F1 expression and negative correlations between miR-4490 and USP22 as well as miR-4490 and POU2F1 expression in primary GC tissues. Based on our results we conclude that miR-4490 acts as a tumor suppressor, and that the POU2F1/miR-4490/USP22 axis plays an important role in the regulation of growth, invasion and EMT of GC cells.
      datePublished:2020-08-28T00:00:00Z
      dateModified:2022-12-29T00:00:00Z
      pageStart:1017
      pageEnd:1033
      license:http://creativecommons.org/licenses/by/4.0/
      sameAs:https://doi.org/10.1007/s13402-020-00553-1
      keywords:
         Gastric cancer
         Metastasis
         MiR-4490
         POU2F1
         Epithelial-mesenchymal transition
         Cancer Research
         Biomedicine
         general
         Pathology
         Oncology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs13402-020-00553-1/MediaObjects/13402_2020_553_Fig1_HTML.png
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      isPartOf:
         name:Cellular Oncology
         issn:
            2211-3436
            2211-3428
         volumeNumber:43
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Netherlands
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Yizhi Xiao
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Side Liu
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
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                  name:Longgang District People’s Hospital
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                     name:Department of Gastroenterology, Longgang District People’s Hospital, Shenzhen, China
                     type:PostalAddress
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            name:Jiaying Li
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
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                  name:Nanfang Hospital, Southern Medical University
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                     name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
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            name:Weimei Tang
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                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
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                  address:
                     name:Department of Gastroenterology, Longgang District People’s Hospital, Shenzhen, China
                     type:PostalAddress
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            name:Wenjing Zhang
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                  name:the First people’s Hospital of Yunnan Province, Medical School of Kunming University of Science and Technology
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                     name:Department of Medical Oncology, the First people’s Hospital of Yunnan Province, Medical School of Kunming University of Science and Technology, Kunming, China
                     type:PostalAddress
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            type:Person
            name:Jianjiao Lin
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
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                  name:Longgang District People’s Hospital
                  address:
                     name:Department of Gastroenterology, Longgang District People’s Hospital, Shenzhen, China
                     type:PostalAddress
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            type:Person
            name:Jing Wang
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
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            name:Xiaosheng Wu
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Department of Digestive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Guangnan Liu
            affiliation:
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