We are analyzing https://link.springer.com/article/10.1007/s13238-022-00913-7.

Title:
Connecting past and present: single-cell lineage tracing | Protein & Cell
Description:
Central to the core principle of cell theory, depicting cells’ history, state and fate is a fundamental goal in modern biology. By leveraging clonal analysis and single-cell RNA-seq technologies, single-cell lineage tracing provides new opportunities to interrogate both cell states and lineage histories. During the past few years, many strategies to achieve lineage tracing at single-cell resolution have been developed, and three of them (integration barcodes, polylox barcodes, and CRISPR barcodes) are noteworthy as they are amenable in experimentally tractable systems. Although the above strategies have been demonstrated in animal development and stem cell research, much care and effort are still required to implement these methods. Here we review the development of single-cell lineage tracing, major characteristics of the cell barcoding strategies, applications, as well as technical considerations and limitations, providing a guide to choose or improve the single-cell barcoding lineage tracing.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We find it hard to spot revenue streams.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com might be making money, but it's not detectable how they're doing it.

Keywords {🔍}

cell, lineage, barcodes, pubmed, article, cas, google, scholar, cells, tracing, singlecell, barcoding, central, integration, development, clonal, crispr, stem, analysis, barcode, nat, information, multiple, types, polylox, sequencing, dna, molecular, fig, fate, methods, tree, sites, human, developmental, single, sclt, klein, targets, random, nature, current, gene, progenitors, genetic, mutations, editing, insertion, mouse, trees,

Topics {✒️}

single-cell temporal-omics approaches hepato-pancreato-biliary progenitor niche single-cell rna-seq technologies single-cell rna-seq survey article download pdf parallel single-cell rna-seq machine-learning-based approaches leverage human stem-cell-derived organoids crispr-cas9-induced genetic scars crispr/cas9-mediated gene editing maximum-parsimony-based methods search single-cell time-resolved model cell-type-based lineage trees combines single-cell omics cre-recombinase-driven polylox barcoding reach single-cell resolution dna double-strand breaks single-cell rna sequencing single-cell rna-seq machine-learning-based amberland fluorescence-activated cell sorting single-cell sequencing data single-cell transcriptional landscape single-cell lineage tracing surrounding transcription-repressive elements single-cell transcriptomes connected single-cell transcriptomic analysis commercial scrna-seq platforms cas9-expressing mouse lines single-cell transcriptome exploration expensive long-read sequencing single-cell molecular map high-throughput dense reconstruction scrna-seq data continue scaling single-cell genomics systematic single-cell evaluation gene expression profiles activation-induced cytidine deaminase high-throughput sequencing methods distance-based methods aim crispr-based genome editing post-mitotic editing events simultaneous single-cell profiling base-editing barcodes comprised cas key laboratory endogenous crispr/cas9 arrays ground-truth cellular history maximum-parsimony-based cassiopeia maximum-likelihood-based methods single-cell mapping

Questions {❓}

Egli D, Zuccaro MV, Kosicki M, Church GM, Bradley A, Jasin M (2018) Inter-homologue repair in fertilized human eggs?
Salvador-Martinez I, Grillo M, Averof M, Telford MJ (2019) Is it possible to reconstruct an accurate cell lineage using CRISPR recorders?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Connecting past and present: single-cell lineage tracing
         description:Central to the core principle of cell theory, depicting cells’ history, state and fate is a fundamental goal in modern biology. By leveraging clonal analysis and single-cell RNA-seq technologies, single-cell lineage tracing provides new opportunities to interrogate both cell states and lineage histories. During the past few years, many strategies to achieve lineage tracing at single-cell resolution have been developed, and three of them (integration barcodes, polylox barcodes, and CRISPR barcodes) are noteworthy as they are amenable in experimentally tractable systems. Although the above strategies have been demonstrated in animal development and stem cell research, much care and effort are still required to implement these methods. Here we review the development of single-cell lineage tracing, major characteristics of the cell barcoding strategies, applications, as well as technical considerations and limitations, providing a guide to choose or improve the single-cell barcoding lineage tracing.
         datePublished:2022-04-19T00:00:00Z
         dateModified:2022-04-19T00:00:00Z
         pageStart:790
         pageEnd:807
         license:http://creativecommons.org/licenses/by/4.0/
         sameAs:https://doi.org/10.1007/s13238-022-00913-7
         keywords:
            single-cell lineage tracing
            integration barcodes
            polylox barcodes
            CRISPR barcodes
            Biochemistry
            general
            Protein Science
            Cell Biology
            Stem Cells
            Human Genetics
            Developmental Biology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs13238-022-00913-7/MediaObjects/13238_2022_913_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs13238-022-00913-7/MediaObjects/13238_2022_913_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs13238-022-00913-7/MediaObjects/13238_2022_913_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs13238-022-00913-7/MediaObjects/13238_2022_913_Fig4_HTML.png
         isPartOf:
            name:Protein & Cell
            issn:
               1674-8018
            volumeNumber:13
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Higher Education Press
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Cheng Chen
               url:https://orcid.org/0000-0002-4723-2525
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yuanxin Liao
               url:https://orcid.org/0000-0003-1508-9936
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
                        type:PostalAddress
                     type:Organization
                     name:University of Chinese Academy of Sciences
                     address:
                        name:University of Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Guangdun Peng
               url:https://orcid.org/0000-0002-8586-0637
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
                        type:PostalAddress
                     type:Organization
                     name:Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory)
                     address:
                        name:Center for Cell Lineage and Atlas, Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China
                        type:PostalAddress
                     type:Organization
                     name:Chinese Academy of Sciences
                     address:
                        name:Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Connecting past and present: single-cell lineage tracing
      description:Central to the core principle of cell theory, depicting cells’ history, state and fate is a fundamental goal in modern biology. By leveraging clonal analysis and single-cell RNA-seq technologies, single-cell lineage tracing provides new opportunities to interrogate both cell states and lineage histories. During the past few years, many strategies to achieve lineage tracing at single-cell resolution have been developed, and three of them (integration barcodes, polylox barcodes, and CRISPR barcodes) are noteworthy as they are amenable in experimentally tractable systems. Although the above strategies have been demonstrated in animal development and stem cell research, much care and effort are still required to implement these methods. Here we review the development of single-cell lineage tracing, major characteristics of the cell barcoding strategies, applications, as well as technical considerations and limitations, providing a guide to choose or improve the single-cell barcoding lineage tracing.
      datePublished:2022-04-19T00:00:00Z
      dateModified:2022-04-19T00:00:00Z
      pageStart:790
      pageEnd:807
      license:http://creativecommons.org/licenses/by/4.0/
      sameAs:https://doi.org/10.1007/s13238-022-00913-7
      keywords:
         single-cell lineage tracing
         integration barcodes
         polylox barcodes
         CRISPR barcodes
         Biochemistry
         general
         Protein Science
         Cell Biology
         Stem Cells
         Human Genetics
         Developmental Biology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs13238-022-00913-7/MediaObjects/13238_2022_913_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs13238-022-00913-7/MediaObjects/13238_2022_913_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs13238-022-00913-7/MediaObjects/13238_2022_913_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs13238-022-00913-7/MediaObjects/13238_2022_913_Fig4_HTML.png
      isPartOf:
         name:Protein & Cell
         issn:
            1674-8018
         volumeNumber:13
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Higher Education Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Cheng Chen
            url:https://orcid.org/0000-0002-4723-2525
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yuanxin Liao
            url:https://orcid.org/0000-0003-1508-9936
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
                     type:PostalAddress
                  type:Organization
                  name:University of Chinese Academy of Sciences
                  address:
                     name:University of Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Guangdun Peng
            url:https://orcid.org/0000-0002-8586-0637
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
                     type:PostalAddress
                  type:Organization
                  name:Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory)
                  address:
                     name:Center for Cell Lineage and Atlas, Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China
                     type:PostalAddress
                  type:Organization
                  name:Chinese Academy of Sciences
                  address:
                     name:Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Protein & Cell
      issn:
         1674-8018
      volumeNumber:13
Organization:
      name:Higher Education Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Chinese Academy of Sciences
      address:
         name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
         type:PostalAddress
      name:Chinese Academy of Sciences
      address:
         name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
         type:PostalAddress
      name:University of Chinese Academy of Sciences
      address:
         name:University of Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:Chinese Academy of Sciences
      address:
         name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
         type:PostalAddress
      name:Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory)
      address:
         name:Center for Cell Lineage and Atlas, Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China
         type:PostalAddress
      name:Chinese Academy of Sciences
      address:
         name:Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Cheng Chen
      url:https://orcid.org/0000-0002-4723-2525
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Yuanxin Liao
      url:https://orcid.org/0000-0003-1508-9936
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
               type:PostalAddress
            type:Organization
            name:University of Chinese Academy of Sciences
            address:
               name:University of Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      name:Guangdun Peng
      url:https://orcid.org/0000-0002-8586-0637
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
               type:PostalAddress
            type:Organization
            name:Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory)
            address:
               name:Center for Cell Lineage and Atlas, Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China
               type:PostalAddress
            type:Organization
            name:Chinese Academy of Sciences
            address:
               name:Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
      name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
      name:University of Chinese Academy of Sciences, Beijing, China
      name:Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
      name:Center for Cell Lineage and Atlas, Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China
      name:Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China

External Links {🔗}(338)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

5.8s.

LINK . SPRINGER . COM {}