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Title:
Downregulation of E-cadherin expression in breast cancer by promoter hypermethylation and its relation with progression and prognosis of tumor | Medical Oncology
Description:
Breast cancer is the most common cancer in women around the world, and novel prognosis strategies is needed to control more accurate and effective of this malignant disease. Among the latest prognostic markers is E-cadherin, which mediates cell–cell adhesion by associating with catenins. Loss of E-cadherin gene (CDH1) function by genetic or epigenetic alteration leads to tumorigenesis. The aim of our study was to investigate E-cadherin gene promoter methylation in breast cancer, and its correlation with E-cadherin protein expression. Fifty primary breast cancers tissue with ductal type and 50 normal breast sample from the same patients that was located adjacent to tumor region as controls were provided by Imam Reza-based referral and teaching hospital affiliated to Tabriz University of Medical Sciences, Tabriz, Iran. CDH1 promoter region CpG sites methylation and E-cadherin protein expression were determined by bisulfite-specific polymerase chain reaction and Western blot analysis, and the resulting products were sequenced on an ABI automated sequencer for firm conclusion. CDH1 hypermethylation in breast tumor specimen (ductal type) was observed in 94 % (47 of 50) comparing with normal samples methylation, and the significant difference was (p = 0.000). Protein expression in tumor samples tends to diminish with the CDH1 promoter region methylation. In the group of 50 ductal carcinomas cases, most of the cases showing CDH1 hypermethylation correlated inversely with the reduced levels of expression of E-cadherin proteins (95 % of full-methylated tumor samples had no protein expression, and 4.5 % of them had weak expression levels). Possible association was observed between CDH1 methylation and its protein expression (p = 0.000). The results of methylation analysis in promoter region in ten CpG sites (863, 865, 873, 879, 887, 892, 901, 918, 920, and 940) suggested that abnormal CDH1 methylation occurs in high frequencies in ductal breast tumors probably sounds the process of carcinogenesis progression.
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Keywords {🔍}
article, pubmed, cancer, ecadherin, google, scholar, breast, cas, expression, methylation, promoter, cdh, protein, iran, central, medical, hypermethylation, tumor, gene, university, region, cpg, analysis, research, access, carcinoma, cell, department, privacy, cookies, content, progression, human, tehran, publish, search, downregulation, prognosis, september, genetic, epigenetic, ductal, dna, oncol, islamic, data, information, log, journal, shargh,
Topics {✒️}
month download article/chapter imam reza-based referral epithelial reverting transition pre-malignant epithelial cells mutant e-cadherin variant persistant e-cadherin expression mediates cell–cell adhesion e-cadherin protein expression full-methylated tumor samples full article pdf dna methylation reveals e-cadherin gene ovarian cancer cells normal samples methylation e-cadherin expression 2023 e-cadherin expression expression e-cadherin e-cadherin protein ten cpg sites e-cadherin proteins privacy choices/manage cookies breast cancer phenotype infiltrating breast cancer inflammatory breast cancer breast carcinoma cell methylated-cpg islands ductal breast tumors human bladder cancer article shargh cell-cell contact breast tumor specimen related subjects ribeiro-filho weak expression levels human dnmt3l protein natl cancer inst breast cancer patients tumor suppressor genes renal cell carcinoma islamic azad university cpg methylation human hepatocellular carcinoma clin cancer res cdh1 promoter hypermethylation estrogen receptor expression european economic area abi automated sequencer tissue microarray study genome-wide profiling mapk/erk signaling
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headline:Downregulation of E-cadherin expression in breast cancer by promoter hypermethylation and its relation with progression and prognosis of tumor
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Breast cancer
Methylation
E-cadherin
Progression
Prognosis
Oncology
Hematology
Pathology
Internal Medicine
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Methylation
E-cadherin
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Hematology
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