We are analyzing https://link.springer.com/article/10.1007/s12015-018-9821-1.

Title:
VSELs Maintain their Pluripotency and Competence to Differentiate after Enhanced Ex Vivo Expansion | Stem Cell Reviews and Reports
Description:
The very small embryonic-like stem cells (VSELs) are known as a subset of adult pluripotent stem cells able to differentiate to all three germ layers. However, their small number and quiescence restrict the possibility of their use in cell therapy. In the present study, we first delineate different subpopulation of VSELs from human cord blood CD34+ cells to define their purity. We next determine genes expression levels in the whole transcriptome of VSELs expressing the pluripotent marker NANOG and control cells under the steady state condition. We found that more than a thousand of genes are downregulated in VSELs, as well as many membrane receptors, cells signaling molecules and CDKs mRNAs. In addition, we observed discordance in some pluripotent genes expression levels with embryonic stem cells (ESCs), which could explain VSELs quiescence. We then evaluate VSELs capacity to expand and differentiate in vitro in specific and appropriate media. After 12 days culture in specific medium containing a pyrimidoindole derivative (UM171), VSELs were significantly expanded for the first time without feeder cells and importantly preserve their capacities to differentiate into hematopoietic and endothelial cells. Interestingly, this stimulation of VSELs self-renewal restores the expression of some downregulated genes known as key regulators of cell proliferation and differentiation. The properties of such pluripotent expanded cells make them a potential candidate in regenerative medicine.
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Keywords {🔍}

cells, vsels, stem, pubmed, expression, cell, genes, article, google, scholar, cas, nanog, differentiation, fig, control, expansion, days, small, human, culture, blood, medium, analysis, gene, ratajczak, embryoniclike, pluripotency, differentiate, embryonic, markers, media, pluripotent, hematopoietic, lincd, central, bone, cord, expanded, data, quiescence, expressing, cxcr, time, kucia, mrna, study, day, observed, proliferation, ucb,

Topics {✒️}

sorted lin-cd34 + cd45-nanog+ vsels viable lin-cd34+cd45-cd133+ lin-cd34 + cd45-cd133 + nanog+ lin-cd34+cd45− nanog+ cells lin-cd34 + cd45-nanog+cxcr4+ lin-cd34 + cd45- expressing cd133+ $$ {\displaystyle \begin{array}{ lin-cd34 + cd45-cd133 + cxcr4+ sorted lin-cd34 + cd45- cells lin-cd34 + cd45-cd133+ vsels bone marrow-derived oct-4+cxcr4+ssea-1+ lin-cd34 + cd45- cells lin-cd34+cd45-cxcr4+ paired-end vsels cd34 + cd45-cd133+ shows cord blood lin nanog mrna multi-labeling lin- cd34+ cells expanded bone marrow-derived lin-cd45- cells quiescent bone marrow-residing tests showed real time rt-pcr umbilical cord blood asymmetric/symmetric cell divisions article download pdf hyaluronic acid-rich node clonogenic progenitor assays bars represent average ± sd illumina miseq-qc run c2c12 supportive cell-line flow cytometry sorting immuno-magnetic negative selection human cord blood cd34 + cd45- cells um171-treated cells encode bone-marrow-derived bone marrow-derived vivo mesenchymal-cell coculture stem cell factor full size image epcr/cd201/procr cd34+ stem cells cord-blood engraftment mobilized cd34+ cells cell cycle determination nanog mrna labeling m-values normalization method murine bone marrowderived adult bone marrow

Schema {🗺️}

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         description:The very small embryonic-like stem cells (VSELs) are known as a subset of adult pluripotent stem cells able to differentiate to all three germ layers. However, their small number and quiescence restrict the possibility of their use in cell therapy. In the present study, we first delineate different subpopulation of VSELs from human cord blood CD34+ cells to define their purity. We next determine genes expression levels in the whole transcriptome of VSELs expressing the pluripotent marker NANOG and control cells under the steady state condition. We found that more than a thousand of genes are downregulated in VSELs, as well as many membrane receptors, cells signaling molecules and CDKs mRNAs. In addition, we observed discordance in some pluripotent genes expression levels with embryonic stem cells (ESCs), which could explain VSELs quiescence. We then evaluate VSELs capacity to expand and differentiate in vitro in specific and appropriate media. After 12 days culture in specific medium containing a pyrimidoindole derivative (UM171), VSELs were significantly expanded for the first time without feeder cells and importantly preserve their capacities to differentiate into hematopoietic and endothelial cells. Interestingly, this stimulation of VSELs self-renewal restores the expression of some downregulated genes known as key regulators of cell proliferation and differentiation. The properties of such pluripotent expanded cells make them a potential candidate in regenerative medicine.
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      headline:VSELs Maintain their Pluripotency and Competence to Differentiate after Enhanced Ex Vivo Expansion
      description:The very small embryonic-like stem cells (VSELs) are known as a subset of adult pluripotent stem cells able to differentiate to all three germ layers. However, their small number and quiescence restrict the possibility of their use in cell therapy. In the present study, we first delineate different subpopulation of VSELs from human cord blood CD34+ cells to define their purity. We next determine genes expression levels in the whole transcriptome of VSELs expressing the pluripotent marker NANOG and control cells under the steady state condition. We found that more than a thousand of genes are downregulated in VSELs, as well as many membrane receptors, cells signaling molecules and CDKs mRNAs. In addition, we observed discordance in some pluripotent genes expression levels with embryonic stem cells (ESCs), which could explain VSELs quiescence. We then evaluate VSELs capacity to expand and differentiate in vitro in specific and appropriate media. After 12 days culture in specific medium containing a pyrimidoindole derivative (UM171), VSELs were significantly expanded for the first time without feeder cells and importantly preserve their capacities to differentiate into hematopoietic and endothelial cells. Interestingly, this stimulation of VSELs self-renewal restores the expression of some downregulated genes known as key regulators of cell proliferation and differentiation. The properties of such pluripotent expanded cells make them a potential candidate in regenerative medicine.
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         Regenerative Medicine/Tissue Engineering
         Cell Biology
         Biomedical Engineering and Bioengineering
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      name:Hôpital du Hasenrain, Institut de Recherche en Hématologie et Transplantation (IRHT), Mulhouse, France
      name:Hôpital du Hasenrain, Institut de Recherche en Hématologie et Transplantation (IRHT), Mulhouse, France
      name:Hôpital du Hasenrain, Institut de Recherche en Hématologie et Transplantation (IRHT), Mulhouse, France
      name:Hôpital du Hasenrain, Institut de Recherche en Hématologie et Transplantation (IRHT), Mulhouse, France
      name:Hôpital du Hasenrain, Institut de Recherche en Hématologie et Transplantation (IRHT), Mulhouse, France

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