We are analyzing https://link.springer.com/article/10.1007/s12015-016-9670-8.

Title:
Cellular GFP Toxicity and Immunogenicity: Potential Confounders in in Vivo Cell Tracking Experiments | Stem Cell Reviews and Reports
Description:
Green Fluorescent protein (GFP), used as a cellular tag, provides researchers with a valuable method of measuring gene expression and cell tracking. However, there is evidence to suggest that the immunogenicity and cytotoxicity of GFP potentially confounds the interpretation of in vivo experimental data. Studies have shown that GFP expression can deteriorate over time as GFP tagged cells are prone to death. Therefore, the cells that were originally marked with GFP do not survive and cannot be accurately traced over time. This review will present current evidence for the immunogenicity and cytotoxicity of GFP in in vivo studies by characterizing these responses.
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Keywords {🔍}

gfp, cells, pubmed, article, google, scholar, cell, cas, protein, fluorescent, mice, immunogenicity, cellular, expression, response, egfp, green, gene, proteins, immune, hepatocytes, tracking, vivo, stem, cytotoxicity, apoptosis, journal, death, cytotoxic, experiments, liver, transfected, central, cpp, toxicity, wildtype, mhc, transplantation, therapy, nature, compared, transplanted, rat, research, molecular, structure, damage, animal, class, leukemia,

Topics {✒️}

bone-marrow-derived cells bone marrow-derived immune h2-kd-restricted ctl epitope interleukin-1β converting enzyme gfp-expressing wild-type animal article download pdf hendrix burn/wound laboratory wild-type leukemia cells t-cell inhibitor agent blue light-induced reactivity establishes long-term expression wild-type hepatocytes transplanted gfp-induced hepatocytes decreased stem/progenitor cells cell biology/editorial board egfp-expressing leukemia cells empty-vector transfected cells egfp-expressing el-4 cells strain-specific disease susceptibility lack functional t-cells adult stem cells cytotoxic t-lymphocyte recognition immunodominant h-2d ctl green fluorescence protein bone marrow transplantation cd80-transduced neuroblastoma cells egfp-transduced cells administered gfp-transgenic rat livers gfp-labeled cells including transplanted gfp-positive hepatocytes gfp-positive hepatocytes led gfp-positive hepatocytes transplanted gfp-transduced cells due stem cell rev respiratory syncytial virus gfp-labeled cells results actin-myosin interaction major histocompatibility complex wild-type rats wild-type hepatocytes live-cell microscopy green fluorescent protein p53-dependent glutathione privacy choices/manage cookies t-cell immunity adult hepatocytes transduced wild-type gfp hepatic cells transfected reactive oxygen species oxygen-reactive species

Questions {❓}

Is green fluorescent protein toxic to the living cells?

Schema {🗺️}

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         description:Green Fluorescent protein (GFP), used as a cellular tag, provides researchers with a valuable method of measuring gene expression and cell tracking. However, there is evidence to suggest that the immunogenicity and cytotoxicity of GFP potentially confounds the interpretation of in vivo experimental data. Studies have shown that GFP expression can deteriorate over time as GFP tagged cells are prone to death. Therefore, the cells that were originally marked with GFP do not survive and cannot be accurately traced over time. This review will present current evidence for the immunogenicity and cytotoxicity of GFP in in vivo studies by characterizing these responses.
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      headline:Cellular GFP Toxicity and Immunogenicity: Potential Confounders in in Vivo Cell Tracking Experiments
      description:Green Fluorescent protein (GFP), used as a cellular tag, provides researchers with a valuable method of measuring gene expression and cell tracking. However, there is evidence to suggest that the immunogenicity and cytotoxicity of GFP potentially confounds the interpretation of in vivo experimental data. Studies have shown that GFP expression can deteriorate over time as GFP tagged cells are prone to death. Therefore, the cells that were originally marked with GFP do not survive and cannot be accurately traced over time. This review will present current evidence for the immunogenicity and cytotoxicity of GFP in in vivo studies by characterizing these responses.
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         Biomedical Engineering and Bioengineering
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