We are analyzing https://link.springer.com/article/10.1007/s12013-015-0700-y.

Title:
Estrogen Receptor Alpha (ERα)-Associated Fibroblasts Promote Cell Growth in Prostate Cancer | Cell Biochemistry and Biophysics
Description:
Estrogen receptor (ER) is expressed in cancer-associated fibroblasts (CAFs) in the stromal compartment of cancerous prostate. However, the effect of ERα in CAF cells on prostate cancer (PCa) cell growth remains unclear. We used lentiviral transduction to stably express ERα in CAF cells isolated from transgenic adenocarcinoma of the mouse prostate model. MTT and 3D colony-formation assays demonstrated that conditioned medium from ERα-expressing CAF cells (CAF-ERα+) promoted cell proliferation and colony growth of various PCa cell lines, such as PC3, LNCaP, 22RV1, and C4-2. We further confirmed the in vitro data by orthotopically co-implanting 22RV1, transfected with firefly luciferase, and CAF-ERα+ cells in vivo using mouse model. Mice co-implanted with CAF-ERα+ exhibited stronger luciferase signals and bigger tumor size compared to animals co-implanted with CAF that do not express ER. Our results demonstrate that ER expressed in CAF might play a pro-proliferative role in PCa.
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Keywords {🔍}

article, pubmed, cancer, google, scholar, prostate, cas, journal, estrogen, cell, cells, receptor, fibroblasts, prostatic, endocrinology, research, stromal, human, access, central, cancerassociated, privacy, cookies, content, alpha, growth, wang, caf, mice, role, stroma, normal, tissue, data, information, publish, search, biochemistry, zhong, tumor, open, development, clinical, pathology, author, log, promote, jun, pca, mouse,

Topics {✒️}

month download article/chapter mujun lu & zhong wang sex hormone-induced hyperplasia pro-inflammatory cytokines/chemokines article cell biochemistry altered tgf-beta signaling cancer stem cells erα-expressing caf cells androgen receptor signalling prostate epithelium derived related subjects estrogen receptor alpha full article pdf prostatic stromal cells cancer cell invasion human prostate cancer endocrine-related cancer prostate cancer cells privacy choices/manage cookies carcinomatous human prostate estrogen receptor α estrogen receptor expression promoted cell proliferation estrogen receptors alpha estrogen receptor response prostatic hormonal carcinogenesis estrogens promote invasion mouse prostate model situ estrogen production caf cells isolated cd90hi prostate cancer tgf-beta signaling premalignant prostatic lesions caf-erα+ cells suppressed prostate tumourigenesis aromatase-deficient mice estrogen-initiated transformation pca cell lines author correspondence article da luminal breast cancer anti-cancer therapy national cancer institute clinical cancer research murine prostate gland tumor-promoting phenotype prostate cancer progression squamous metaplasia evident conditions privacy policy shanghai ninth people

Questions {❓}

Tumour–stroma interaction: Cancer-associated fibroblasts as novel targets in anti-cancer therapy?

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         description:Estrogen receptor (ER) is expressed in cancer-associated fibroblasts (CAFs) in the stromal compartment of cancerous prostate. However, the effect of ERα in CAF cells on prostate cancer (PCa) cell growth remains unclear. We used lentiviral transduction to stably express ERα in CAF cells isolated from transgenic adenocarcinoma of the mouse prostate model. MTT and 3D colony-formation assays demonstrated that conditioned medium from ERα-expressing CAF cells (CAF-ERα+) promoted cell proliferation and colony growth of various PCa cell lines, such as PC3, LNCaP, 22RV1, and C4-2. We further confirmed the in vitro data by orthotopically co-implanting 22RV1, transfected with firefly luciferase, and CAF-ERα+ cells in vivo using mouse model. Mice co-implanted with CAF-ERα+ exhibited stronger luciferase signals and bigger tumor size compared to animals co-implanted with CAF that do not express ER. Our results demonstrate that ER expressed in CAF might play a pro-proliferative role in PCa.
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      headline:Estrogen Receptor Alpha (ERα)-Associated Fibroblasts Promote Cell Growth in Prostate Cancer
      description:Estrogen receptor (ER) is expressed in cancer-associated fibroblasts (CAFs) in the stromal compartment of cancerous prostate. However, the effect of ERα in CAF cells on prostate cancer (PCa) cell growth remains unclear. We used lentiviral transduction to stably express ERα in CAF cells isolated from transgenic adenocarcinoma of the mouse prostate model. MTT and 3D colony-formation assays demonstrated that conditioned medium from ERα-expressing CAF cells (CAF-ERα+) promoted cell proliferation and colony growth of various PCa cell lines, such as PC3, LNCaP, 22RV1, and C4-2. We further confirmed the in vitro data by orthotopically co-implanting 22RV1, transfected with firefly luciferase, and CAF-ERα+ cells in vivo using mouse model. Mice co-implanted with CAF-ERα+ exhibited stronger luciferase signals and bigger tumor size compared to animals co-implanted with CAF that do not express ER. Our results demonstrate that ER expressed in CAF might play a pro-proliferative role in PCa.
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