We are analyzing https://link.springer.com/article/10.1007/s11427-012-4309-8.

Title:
Epigenetic reprogramming of embryos derived from sperm frozen at −20°C | Science China Life Sciences
Description:
Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after application of intracytoplasmic sperm injection (ICSI) is much needed. It has been shown previously that spermatozoa frozen at −20°C can activate oocytes and support full-term embryo development. However, epigenetic reprogramming could be affected by the environment and by the in vitro manipulation of gametes. Here, we investigated embryo epigenetic reprogramming including DNA methylation and histone modification, in embryos derived from sperm preserved at −20°C without cryoprotectants. The results showed that although both fertilization and embryo developmental competence were decreased, the dynamic epigenetic reprogramming of embryos derived from frozen sperm was similar to the reprogramming of embryos derived from fresh sperm. The results reported in this study indicate that sperm frozen without cryoprotectant is epigenetically safe for ICSI.
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Keywords {🔍}

pubmed, article, google, scholar, cas, sperm, spermatozoa, biol, reprod, mouse, development, epigenetic, reprogramming, embryos, frozen, intracytoplasmic, injection, dna, methylation, central, oocytes, embryo, histone, sci, yanagimachi, china, access, freezedried, dev, privacy, cookies, research, derived, open, cryopreservation, vitro, nature, offspring, mice, content, information, publish, search, science, download, chao, animal, icsi, human, freezing,

Topics {✒️}

china life sci ca2+ oscillation-inducing abilities zona-free hamster ova article download pdf species-dependent expression patterns freeze-dried mouse spermatozoa privacy choices/manage cookies dynamic epigenetic reprogramming bmc dev biol cyclical dna methylation sanchez-partida histone h3 methylation histone lysine methylation early mouse embryo paternal dna packaging long-term outcomes dna methyltransferase genes article chao histone modification inadequate histone deacetylation main content log long-term storage zygotic paternal genome open access search search freeze-dried spermatozoa full-term development guolan gao frozen reproductive organs nuclear dna integrity european economic area somatic nuclear transfer transcriptionally active promoter lipid bilayer vesicles biochim biophys acta embryo developmental competence conditions privacy policy freeze-drying solution epigenetic reprogramming intracytoplasmic sperm injection rat embryos developed aviation general hospital mouse sperm desiccated mol reprod dev full access reprod biomed online accepting optional cookies hybrid mouse strains biomedical research related subjects

Questions {❓}

Paternal DNA packaging in spermatozoa: more than the sum of its parts?

Schema {🗺️}

WebPage:
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         headline:Epigenetic reprogramming of embryos derived from sperm frozen at −20°C
         description:Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after application of intracytoplasmic sperm injection (ICSI) is much needed. It has been shown previously that spermatozoa frozen at −20°C can activate oocytes and support full-term embryo development. However, epigenetic reprogramming could be affected by the environment and by the in vitro manipulation of gametes. Here, we investigated embryo epigenetic reprogramming including DNA methylation and histone modification, in embryos derived from sperm preserved at −20°C without cryoprotectants. The results showed that although both fertilization and embryo developmental competence were decreased, the dynamic epigenetic reprogramming of embryos derived from frozen sperm was similar to the reprogramming of embryos derived from fresh sperm. The results reported in this study indicate that sperm frozen without cryoprotectant is epigenetically safe for ICSI.
         datePublished:2012-06-15T00:00:00Z
         dateModified:2012-06-15T00:00:00Z
         pageStart:349
         pageEnd:357
         license:https://creativecommons.org/licenses/by/2.0
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            histone modification
            DNA methylation
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ScholarlyArticle:
      headline:Epigenetic reprogramming of embryos derived from sperm frozen at −20°C
      description:Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after application of intracytoplasmic sperm injection (ICSI) is much needed. It has been shown previously that spermatozoa frozen at −20°C can activate oocytes and support full-term embryo development. However, epigenetic reprogramming could be affected by the environment and by the in vitro manipulation of gametes. Here, we investigated embryo epigenetic reprogramming including DNA methylation and histone modification, in embryos derived from sperm preserved at −20°C without cryoprotectants. The results showed that although both fertilization and embryo developmental competence were decreased, the dynamic epigenetic reprogramming of embryos derived from frozen sperm was similar to the reprogramming of embryos derived from fresh sperm. The results reported in this study indicate that sperm frozen without cryoprotectant is epigenetically safe for ICSI.
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      dateModified:2012-06-15T00:00:00Z
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         spermatozoa freezing
         ICSI
         epigenetic
         histone modification
         DNA methylation
         Life Sciences
         general
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      name:Center of Reproductive Medicine, the First Affiliated Hospital of Nanchang University, Nanchang, China
      name:Center of Reproductive Medicine, the First Affiliated Hospital of Nanchang University, Nanchang, China
      name:Center of Reproductive Medicine, the First Affiliated Hospital of Nanchang University, Nanchang, China
      name:Department of Obstetrics and Gynecology, Aviation General Hospital of China, Beijing, China

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