We are analyzing https://link.springer.com/article/10.1007/s11274-011-0792-5.

Title:
Functional properties of free and encapsulated Lactobacillus reuteri DPC16 during and after passage through a simulated gastrointestinal tract | World Journal of Microbiology and Biotechnology
Description:
Lactobacillus reuteri DPC16 is a probiotic bacterium that has strong antimicrobial activities on pathogens, mainly due to its ability to produce reuterin, an antimicrobial compound. The objective of this study was to examine the ability of an encapsulation technique to protect the functional properties of cells of L. reuteri DPC16 during passage through a simulated GI tract. The functional properties of the cells were studied before and after passage through the tract. An alginate-skim milk encapsulation system was used to deliver the probiotic bacterium through the simulated GI tract, allowing for the release of the cells into the simulated colonic fluid. The cells were then isolated and cultured. The recovered cells showed no diminution in functional properties, including their growth kinetics, ability to adhere to epithelial cells and ability to inhibit the adhesion of E. coli to epithelial cells. The bacteriostatic and bactericidal properties of the recovered cells against some pathogens were significantly greater (P < 0.05) than those of the original cells. Production of reuterin by the recovered cells was significantly greater than that of the original cells when cultured in MRS medium in the absence of its metabolic precursor, glycerol. The results demonstrate significant consequences for the application of the encapsulation technique to protect and/or enhance the functional properties of the probiotic cells.
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Keywords {🔍}

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Topics {✒️}

ph-responsive pmaa/perlite composite simulated gastro-intestinal conditions time-dependent oral microparticles month download article/chapter sung je lee simulated gastrointestinal tract simulated gastrointestinal fluid lactic acid bacteria alginate matrix coated full article pdf privacy choices/manage cookies lactobacillus reuteri dpc16 related subjects inhibits cell attachment article world journal fructo-oligosaccharide fermentation lactobacillus reuteri jcm1081 simulated gi tract inhibitory activity spectrum conditions privacy policy simulated colonic fluid strong antimicrobial activities van leusden fm optimize budesonide delivery european economic area bacterial adhesion increased stress tolerance outer surface lipoprotein anti-inflammatory factors anti-inflammatory capacity porcine gastric mucin ian maddox article zhao anthony mutukumira lactobacillus plantarum spp antimicrobial activity journal finder publish accepting optional cookies qian zhao lactococcus lactis produced freeze drying enhance check access instant access author information authors reuteri dpc16 lactobacillus reuteri article log produce reuterin recovered cells showed intestinal delivery

Schema {🗺️}

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         description: Lactobacillus reuteri DPC16 is a probiotic bacterium that has strong antimicrobial activities on pathogens, mainly due to its ability to produce reuterin, an antimicrobial compound. The objective of this study was to examine the ability of an encapsulation technique to protect the functional properties of cells of L. reuteri DPC16 during passage through a simulated GI tract. The functional properties of the cells were studied before and after passage through the tract. An alginate-skim milk encapsulation system was used to deliver the probiotic bacterium through the simulated GI tract, allowing for the release of the cells into the simulated colonic fluid. The cells were then isolated and cultured. The recovered cells showed no diminution in functional properties, including their growth kinetics, ability to adhere to epithelial cells and ability to inhibit the adhesion of E. coli to epithelial cells. The bacteriostatic and bactericidal properties of the recovered cells against some pathogens were significantly greater (P < 0.05) than those of the original cells. Production of reuterin by the recovered cells was significantly greater than that of the original cells when cultured in MRS medium in the absence of its metabolic precursor, glycerol. The results demonstrate significant consequences for the application of the encapsulation technique to protect and/or enhance the functional properties of the probiotic cells.
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      headline:Functional properties of free and encapsulated Lactobacillus reuteri DPC16 during and after passage through a simulated gastrointestinal tract
      description: Lactobacillus reuteri DPC16 is a probiotic bacterium that has strong antimicrobial activities on pathogens, mainly due to its ability to produce reuterin, an antimicrobial compound. The objective of this study was to examine the ability of an encapsulation technique to protect the functional properties of cells of L. reuteri DPC16 during passage through a simulated GI tract. The functional properties of the cells were studied before and after passage through the tract. An alginate-skim milk encapsulation system was used to deliver the probiotic bacterium through the simulated GI tract, allowing for the release of the cells into the simulated colonic fluid. The cells were then isolated and cultured. The recovered cells showed no diminution in functional properties, including their growth kinetics, ability to adhere to epithelial cells and ability to inhibit the adhesion of E. coli to epithelial cells. The bacteriostatic and bactericidal properties of the recovered cells against some pathogens were significantly greater (P < 0.05) than those of the original cells. Production of reuterin by the recovered cells was significantly greater than that of the original cells when cultured in MRS medium in the absence of its metabolic precursor, glycerol. The results demonstrate significant consequences for the application of the encapsulation technique to protect and/or enhance the functional properties of the probiotic cells.
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