We are analyzing https://link.springer.com/article/10.1007/s11248-012-9635-x.

Title:
Production of mouse pups from germline transmission-failed knockout chimeras | Transgenic Research
Description:
Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

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         headline:Production of mouse pups from germline transmission-failed knockout chimeras
         description:Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
         datePublished:2012-07-24T00:00:00Z
         dateModified:2012-07-24T00:00:00Z
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      headline:Production of mouse pups from germline transmission-failed knockout chimeras
      description:Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
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         Genetic Engineering
         Molecular Medicine
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