We are analyzing https://link.springer.com/article/10.1007/s11248-007-9100-4.

Title:
Excision of the Frt-flanked neo R cassette from the CD19cre knock-in transgene reduces Cre-mediated recombination | Transgenic Research
Description:
Intercross of mice transgenic for Flp-recombinase with the CD19cre mouse strain leads to excision of the Frt-flanked neo R cassette from the CD19cre knock-in transgene. This significantly reduces the expression level of Cre by the CD19cre transgene and consequently decreases the extent of Cre-mediated recombination of loxP-flanked alleles, most likely due to the fact that this neo R cassette contains polyoma enhancer sequences. We wish to draw attention to this finding, since the Flp-deleter mouse strain is commonly used to remove Frt-flanked selection cassettes in vivo from conditional alleles. Therefore conditional alleles have to be separated from the Flp-deleter transgene by breeding before crosses with CD19cre mice are initiated. In addition our findings suggest that gene expression from the CD19 locus can be increased by the insertion of exogenous enhancer sequences, without compromising B cell specificity.
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Keywords {🔍}

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Topics {✒️}

t-cell-dependent b-cell responses conditional gene-targeted mutation month download article/chapter gene targeting flp-deleter mouse strain cre-mediated recombination article schmidt-supprian marc schmidt-supprian frt-flanked neo gene expression site-directed mutagenesis full article pdf privacy choices/manage cookies cre-mediated mutagenesis polyoma enhancer sequences author information authors flp-deleter transgene b-1 cell development loxp-flanked alleles flp-deleter mice thomas wunderlich targeted insertion european economic area exogenous enhancer sequences diphtheria toxin fragment c-myc-deficient induced cell death pax5/bsap maintains ikappab kinase signaling nih grant ai057947 harvard medical school conditions privacy policy check access instant access schmidt-supprian cd19-deficient mice accepting optional cookies cell depletion due c-myc function author correspondence main content log biomedical research article log journal finder publish hagan rc cd19cre transgene significantly reduces article cite normal cells mice transgenic

Schema {🗺️}

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         headline:Excision of the Frt-flanked neo R cassette from the CD19cre knock-in transgene reduces Cre-mediated recombination
         description:Intercross of mice transgenic for Flp-recombinase with the CD19cre mouse strain leads to excision of the Frt-flanked neo R cassette from the CD19cre knock-in transgene. This significantly reduces the expression level of Cre by the CD19cre transgene and consequently decreases the extent of Cre-mediated recombination of loxP-flanked alleles, most likely due to the fact that this neo R cassette contains polyoma enhancer sequences. We wish to draw attention to this finding, since the Flp-deleter mouse strain is commonly used to remove Frt-flanked selection cassettes in vivo from conditional alleles. Therefore conditional alleles have to be separated from the Flp-deleter transgene by breeding before crosses with CD19cre mice are initiated. In addition our findings suggest that gene expression from the CD19 locus can be increased by the insertion of exogenous enhancer sequences, without compromising B cell specificity.
         datePublished:2007-06-01T00:00:00Z
         dateModified:2007-06-01T00:00:00Z
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             CD19cre mouse stain
            Conditional gene targeting efficiency
            B cells
            Frt-site flanked neomycin resistance gene cassette
            Animal Genetics and Genomics
            Plant Genetics and Genomics
            Transgenics
            Biomedical Engineering/Biotechnology
            Genetic Engineering
            Molecular Medicine
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      headline:Excision of the Frt-flanked neo R cassette from the CD19cre knock-in transgene reduces Cre-mediated recombination
      description:Intercross of mice transgenic for Flp-recombinase with the CD19cre mouse strain leads to excision of the Frt-flanked neo R cassette from the CD19cre knock-in transgene. This significantly reduces the expression level of Cre by the CD19cre transgene and consequently decreases the extent of Cre-mediated recombination of loxP-flanked alleles, most likely due to the fact that this neo R cassette contains polyoma enhancer sequences. We wish to draw attention to this finding, since the Flp-deleter mouse strain is commonly used to remove Frt-flanked selection cassettes in vivo from conditional alleles. Therefore conditional alleles have to be separated from the Flp-deleter transgene by breeding before crosses with CD19cre mice are initiated. In addition our findings suggest that gene expression from the CD19 locus can be increased by the insertion of exogenous enhancer sequences, without compromising B cell specificity.
      datePublished:2007-06-01T00:00:00Z
      dateModified:2007-06-01T00:00:00Z
      pageStart:657
      pageEnd:660
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          CD19cre mouse stain
         Conditional gene targeting efficiency
         B cells
         Frt-site flanked neomycin resistance gene cassette
         Animal Genetics and Genomics
         Plant Genetics and Genomics
         Transgenics
         Biomedical Engineering/Biotechnology
         Genetic Engineering
         Molecular Medicine
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