We are analyzing https://link.springer.com/article/10.1007/s11064-010-0379-9.

Title:
Regulation of Epidermal Growth Factor Receptor Through Interaction of Ganglioside GM3 with GlcNAc of N-Linked Glycan of the Receptor: Demonstration in ldlD Cells | Neurochemical Research
Description:
We investigated interaction of GM3 with N-acetylglucosamine (GlcNAc) termini of N-linked glycans of epidermal growth factor receptor (EGFR), as the underlying mechanism for inhibitory effect of GM3 on EGFR activation, using ldlD cells transfected with EGFR gene. These cells, defective in UDP-Gal/UDP-GalNAc 4-epimerase, are incapable of synthesizing galactose (Gal)-containing glycans, unless Gal is provided in culture (+Gal). Key observations: (1) Expression of GlcNAc termini was high in −Gal cells, and strongly reduced in +Gal cells. (2) Comparative study of inhibitory effect of exogenously-added GM3 on EGFR activation in +Gal versus −Gal cells indicated that higher level of GlcNAc termini on EGFR is correlated with greater inhibitory effect of GM3. (3) GM3-, but not GM1-, coated beads bound to EGFR in lysate of −Gal cells, which have highly exposed GlcNAc termini. Such binding was inhibited in the presence of EDTA, similarly to other carbohydrate-carbohydrate interactions.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Social Networks

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We find it hard to spot revenue streams.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

google, scholar, article, cas, pubmed, cell, receptor, growth, cells, biol, chem, epidermal, factor, interaction, hakomori, glcnac, egfr, handa, usa, ganglioside, gal, research, regulation, termini, access, sci, nlinked, ldld, gene, binding, kinase, proc, natl, acad, tyrosine, privacy, cookies, function, content, guan, phosphorylation, specific, motility, information, publish, search, senitiroh, effect, expression, open,

Topics {✒️}

terminal n-acetyl-d-glucosamine structure n-acetyl-d-glycosamine binding lectin udp-gal/udp-galnac 4-epimerase kazuko handa & sen-itiroh hakomori insulin/transferrin/selenium ldld/egfr month download article/chapter insulin-dependent cell growth n-linked glcnac termini laminin-5-dependent cell motility n-linked glycan related subjects gene transfection-mediated overexpression growth factor binding sen-itiroh hakomori carbohydrate-carbohydrate interactions tyrosine kinase activity carbohydrate-carbohydrate interaction asparagine-linked oligosaccharides o-linked glycosylation full article pdf protein-bound oligosaccharides insulin receptor function ldld cells transfected freire-de-lima receptor-receptor interaction embryonal carcinoma cells n-linked glycans privacy choices/manage cookies hamster cell line tumor cell motility cell growth regulation trends biochem sci regulates receptor function glioma cell line multivalent glcnac termini cell adhesion based tumour cells based allosteric receptor oligomerization erythroagglutinating phytohemagglutinin lectin bladder cancer cells receptor-mediated endocytosis check access instant access cell density-dependent ganglioside-mediated modulation article guan b16 mouse melanoma ldl receptor expression f9 teratocarcinoma cells gm3-mediated interaction

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Regulation of Epidermal Growth Factor Receptor Through Interaction of Ganglioside GM3 with GlcNAc of N-Linked Glycan of the Receptor: Demonstration in ldlD Cells
         description:We investigated interaction of GM3 with N-acetylglucosamine (GlcNAc) termini of N-linked glycans of epidermal growth factor receptor (EGFR), as the underlying mechanism for inhibitory effect of GM3 on EGFR activation, using ldlD cells transfected with EGFR gene. These cells, defective in UDP-Gal/UDP-GalNAc 4-epimerase, are incapable of synthesizing galactose (Gal)-containing glycans, unless Gal is provided in culture (+Gal). Key observations: (1) Expression of GlcNAc termini was high in −Gal cells, and strongly reduced in +Gal cells. (2) Comparative study of inhibitory effect of exogenously-added GM3 on EGFR activation in +Gal versus −Gal cells indicated that higher level of GlcNAc termini on EGFR is correlated with greater inhibitory effect of GM3. (3) GM3-, but not GM1-, coated beads bound to EGFR in lysate of −Gal cells, which have highly exposed GlcNAc termini. Such binding was inhibited in the presence of EDTA, similarly to other carbohydrate-carbohydrate interactions.
         datePublished:2011-01-04T00:00:00Z
         dateModified:2011-01-04T00:00:00Z
         pageStart:1645
         pageEnd:1653
         sameAs:https://doi.org/10.1007/s11064-010-0379-9
         keywords:
            Epidermal growth factor receptor (EGFR)
            ldlD cells
            N-linked glycan
            GlcNAc termini
            Ganglioside GM3
            Carbohydrate-carbohydrate interaction
            Neurosciences
            Neurochemistry
            Biochemistry
            general
            Cell Biology
            Neurology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig5_HTML.gif
         isPartOf:
            name:Neurochemical Research
            issn:
               1573-6903
               0364-3190
            volumeNumber:36
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer US
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Feng Guan
               affiliation:
                     name:Pacific Northwest Research Institute
                     address:
                        name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
                        type:PostalAddress
                     type:Organization
                     name:University of Washington
                     address:
                        name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Kazuko Handa
               affiliation:
                     name:Pacific Northwest Research Institute
                     address:
                        name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
                        type:PostalAddress
                     type:Organization
                     name:University of Washington
                     address:
                        name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Sen-itiroh Hakomori
               affiliation:
                     name:Pacific Northwest Research Institute
                     address:
                        name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
                        type:PostalAddress
                     type:Organization
                     name:University of Washington
                     address:
                        name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Regulation of Epidermal Growth Factor Receptor Through Interaction of Ganglioside GM3 with GlcNAc of N-Linked Glycan of the Receptor: Demonstration in ldlD Cells
      description:We investigated interaction of GM3 with N-acetylglucosamine (GlcNAc) termini of N-linked glycans of epidermal growth factor receptor (EGFR), as the underlying mechanism for inhibitory effect of GM3 on EGFR activation, using ldlD cells transfected with EGFR gene. These cells, defective in UDP-Gal/UDP-GalNAc 4-epimerase, are incapable of synthesizing galactose (Gal)-containing glycans, unless Gal is provided in culture (+Gal). Key observations: (1) Expression of GlcNAc termini was high in −Gal cells, and strongly reduced in +Gal cells. (2) Comparative study of inhibitory effect of exogenously-added GM3 on EGFR activation in +Gal versus −Gal cells indicated that higher level of GlcNAc termini on EGFR is correlated with greater inhibitory effect of GM3. (3) GM3-, but not GM1-, coated beads bound to EGFR in lysate of −Gal cells, which have highly exposed GlcNAc termini. Such binding was inhibited in the presence of EDTA, similarly to other carbohydrate-carbohydrate interactions.
      datePublished:2011-01-04T00:00:00Z
      dateModified:2011-01-04T00:00:00Z
      pageStart:1645
      pageEnd:1653
      sameAs:https://doi.org/10.1007/s11064-010-0379-9
      keywords:
         Epidermal growth factor receptor (EGFR)
         ldlD cells
         N-linked glycan
         GlcNAc termini
         Ganglioside GM3
         Carbohydrate-carbohydrate interaction
         Neurosciences
         Neurochemistry
         Biochemistry
         general
         Cell Biology
         Neurology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11064-010-0379-9/MediaObjects/11064_2010_379_Fig5_HTML.gif
      isPartOf:
         name:Neurochemical Research
         issn:
            1573-6903
            0364-3190
         volumeNumber:36
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Feng Guan
            affiliation:
                  name:Pacific Northwest Research Institute
                  address:
                     name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
                     type:PostalAddress
                  type:Organization
                  name:University of Washington
                  address:
                     name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kazuko Handa
            affiliation:
                  name:Pacific Northwest Research Institute
                  address:
                     name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
                     type:PostalAddress
                  type:Organization
                  name:University of Washington
                  address:
                     name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Sen-itiroh Hakomori
            affiliation:
                  name:Pacific Northwest Research Institute
                  address:
                     name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
                     type:PostalAddress
                  type:Organization
                  name:University of Washington
                  address:
                     name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Neurochemical Research
      issn:
         1573-6903
         0364-3190
      volumeNumber:36
Organization:
      name:Springer US
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Pacific Northwest Research Institute
      address:
         name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
         type:PostalAddress
      name:University of Washington
      address:
         name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
         type:PostalAddress
      name:Pacific Northwest Research Institute
      address:
         name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
         type:PostalAddress
      name:University of Washington
      address:
         name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
         type:PostalAddress
      name:Pacific Northwest Research Institute
      address:
         name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
         type:PostalAddress
      name:University of Washington
      address:
         name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Feng Guan
      affiliation:
            name:Pacific Northwest Research Institute
            address:
               name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
               type:PostalAddress
            type:Organization
            name:University of Washington
            address:
               name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
               type:PostalAddress
            type:Organization
      name:Kazuko Handa
      affiliation:
            name:Pacific Northwest Research Institute
            address:
               name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
               type:PostalAddress
            type:Organization
            name:University of Washington
            address:
               name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
               type:PostalAddress
            type:Organization
      name:Sen-itiroh Hakomori
      affiliation:
            name:Pacific Northwest Research Institute
            address:
               name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
               type:PostalAddress
            type:Organization
            name:University of Washington
            address:
               name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
      name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
      name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
      name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
      name:Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, USA
      name:Departments of Pathobiology and Global Health, University of Washington, Seattle, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(138)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.49s.

LINK . SPRINGER . COM {}